Croyez High Range RNA Ladder

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Unleash the Power of Your Self-Amplifying RNA Research: High Range RNA Ladder

Accurate Size Determination for Long RNA Molecules

The Croyez Bioscience High Range RNA Ladder is your essential tool for precise sizing of self-amplifying RNA (saRNA) and other long RNA molecules during native agarose gel electrophoresis. This pre-stained ladder features nine sharp, precisely defined bands ranging from 200 to 12,000 bases, allowing for accurate visualization of your valuable saRNA samples.

Unparalleled Reliability and Convenience:

Broad Range: Confidently analyze saRNA transcripts and other long RNA molecules with a comprehensive size range.
Pre-stained Bands: Save time and minimize errors with bands ready for direct loading onto your gel.
Exceptional Visualization: Sharp, well-defined bands ensure clear and reliable size determination under UV light.
Compatible Staining Methods: Use traditional ethidium bromide or opt for safer nucleic acid safety dyes like GelRed from Biotium for visualization.

Order yours today and experience the benefits of:

Streamlined Workflow: Reduce preparation time with pre-stained bands.
Confidence in Results: Ensure accurate sizing of your valuable RNA samples.
Versatility: Compatible with various gel electrophoresis protocols.

Specification

Quality Control Testing

The banding pattern of RNA ladder on １% TAE or TBE agarose gels shows clear identifiable bands at each fragment, when stained with nucleic acid safety dye under UV light.

Storage Buffer

1 mM sodium citrate buffer (pH 6.4)

Storage & Stability

This product is stable for 3 months after storage at -80°C and should be avoided from repeated freeze/thaw cycles.

Handling Instruction

For optimal storage, aliquot the reagent into smaller quantities and store at recommended temperature.

Please promptly retrieve the required portion and return it to the appropriate storage temperature.

Recommended to Use

Ladder preparation for Electrophoresis

Mix 1 volume of RNA ladder with 3 volumes of 2X RNA Loading Dye.
Incubate at 65°C for 5 minutes.
Load 0.5 μL of the prepared ladder for every mm of gel lane width (e.g., 4 μL for an 8 mm lane).

Applications

Electrophoresis

Note

To avoid ribonuclease contamination, using RNase-free water is necessary for sample dilution.
We do not recommend use of these markers as a quantitative standard.

Shipping Conditions

Dry ice

Data

The High Range RNA ladder contains various ssRNA sizes up to 12,000 bases, suitable for use on a 1%TAE agarose gel, with different amounts required per lane.