AccuBlue® Broad Range RNA Quantitation Kit
The AccuBlue® Broad Range RNA Quantitation Kit offers the widest linear range (5–1000 ng RNA) of any RNA assay, delivering exceptional RNA selectivity over dsDNA and unmatched sensitivity for NGS and RT-PCR applications. Compatible with both fluorescence microplate readers and the Qubit® fluorometer.
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Widest Linear Range for Accurate RNA Quantitation — Qubit® Compatible, High RNA Selectivity
The AccuBlue® Broad Range RNA Quantitation Kit by Biotium provides the most comprehensive solution for RNA quantitation, combining accuracy, sensitivity, and RNA specificity in a single assay.
With a linear range from 5 ng to 1000 ng RNA, this kit spans both the Qubit® RNA High Sensitivity (HS) and Broad Range (BR) ranges, eliminating the need for multiple kits. Unlike absorbance-based A260 readings or less selective fluorescent assays, AccuBlue® ensures precise results even in the presence of dsDNA contaminants.
The included mammalian RNA standard provides added confidence when quantifying RNA from mammalian sources, making this kit ideal for NGS library prep, RT-PCR, and transcriptomics workflows.
Features
- Widest Linear Range: 5–1000 ng RNA, spanning Qubit® HS & BR ranges.
- Superior RNA Specificity: Highly selective for ssRNA over dsDNA; tolerates equimolar dsDNA contamination.
- High Sensitivity: Accurate quantitation for applications like RNA-seq and RT-PCR.
- Far-Red Emission: Excitation/Emission at 650/670 nm, reducing background interference.
- Flexible Compatibility: Works with fluorescence microplate readers and Qubit® fluorometer (RNA Broad Range program).
- Convenient Standards: Includes mammalian RNA standard (100 ng/µL) for precise calibration.
Kit Components
- RNA Broad Range Dye (200X)
- RNA Broad Range Buffer
- RNA Dilution Buffer (can be used as Qubit® Standard 1)
- RNA Broad Range Standard, 100 ng/µL (can be used as Qubit® Standard 2)
*Replacement vials of RNA standard (cat # 99850) can be purchased separately in case the RNA standard in your kit becomes degraded or otherwise compromised.
Assay Range
The AccuBlue® Broad Range RNA Quantitation Kit delivers a linear detection range of 5–1000 ng RNA per 200 µL assay well, spanning both the Qubit® RNA High Sensitivity (HS) and Broad Range (BR) ranges in a single assay. This broad dynamic range makes it highly versatile and ideal for accurately quantifying RNA in workflows requiring high precision, such as Next-Generation Sequencing (NGS) library preparation and reverse transcription PCR (RT-PCR).
RNA Specificity
Unlike absorbance-based methods (A260) or less selective fluorescent dyes, the RNA Broad Range Dye is highly specific for single-stranded RNA (ssRNA). The assay tolerates the presence of an equimolar amount of double-stranded DNA (dsDNA) without significantly affecting RNA quantitation, reducing errors from contaminating DNA. It is also capable of accurately quantifying short ssRNA species such as miRNA, while producing minimal signal with double-stranded RNA, ensuring reliable and RNA-focused measurements.
Instrument Compatibility
The AccuBlue® Broad Range RNA Quantitation assay is designed for use with fluorescence microplate readers equipped with far-red excitation and emission filters, as well as the Qubit® fluorometer using its pre-programmed RNA Broad Range protocol. For advanced detection, some highly sensitive microplate readers can extend the lower limit of detection to 1 ng RNA (or sample concentrations as low as 0.1 ng/µL), enabling exceptional sensitivity for demanding applications.
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.
✅ For research use only. Not for use in diagnostic procedures.
*Biotium products are available in Singapore and Thailand.
Documentations
Protocols
SDS
Supporting Documents
Product Attributes
Product Attributes
| Shipping condition | Ice pack |
|---|---|
| Storage Conditions | Store at 2 to 8 °C |
| Excitation/Emission | 650/670 nm |
