AccuBlue® High Sensitivity dsDNA Standards, Set of Eight – 0.5 mL each

AccuBlue® High Sensitivity dsDNA Standards, Set of Eight – 0.5 mL each

AccuBlue® High Sensitivity dsDNA Standards, Set of Eight – 0.5 mL each

$89.00

$89.00

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Description

Product Description

Stand-alone set of the eight double-stranded DNA standards provided with the AccuBlue® High Sensitivity dsDNA Quantitation Kit (31006).

Product Description

Stand-alone set of the eight double-stranded DNA standards provided with the AccuBlue® High Sensitivity dsDNA Quantitation Kit (31006).

  • Calf thymus dsDNA in 1X Tris-EDTA
  • Set of eight standards at 0, 0.5, 1, 2, 4, 6, 8 and 10 ng/uL
  • 0.5 mL each

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA Quantitation Kits

dsDNA Quantitation Kit Linear detection range
(dsDNA in assay)
Ex/Em (nm) Suggested Instrument Features
AccuGreen™ High Sensitivity 0.1-100 ng 502/523 Qubit® fluorometer Compare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range 2-1000 ng 500/530 Qubit® fluorometer Compare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen 1 pg-3 ng* 468/507 Fluorescent Plate Reader Most sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity 30 pg-250 ng 468/507 Fluorescent Plate Reader Versatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity 0.2-100 ng 485/530 Fluorescent Plate Reader Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range 2-2000 ng 350/460 Fluorescent Plate Reader Broad linear range with blue fluorescence
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents.

References

1. BMC Oral Health (2014), doi: 10.1186/1472-6831-14-157
2. Infection, Genetics and Evolution(2017), http://dx.doi.org/10.1016/j.meegid.2017.02.006

Product Attributes

Storage Conditions Store at 2 to 8 °C

Protocols