EMBER500™ RNA Prestain Loading Dye

EMBER500™ RNA Prestain Loading Dye

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EMBER500™ RNA Prestain Loading Dye
Human total cellular RNA in water was mixed with EMBER500™ RNA Prestain Loading Dye or loading dye with 250 ug ethidium bromide (EtBr). Samples were heated for 10 minutes at 70°C, then run on a non-denaturing 1% agarose/1X TBE gel. From left to right, loading amounts were 200, 100, 50, or 25 ng/lane for total RNA. Lanes 1-4 used EMBER500™ RNA Prestain Loading Dye, lanes 5-8 used RNA loading dye with EtBr. The gel was imaged using a UVP GelDoc-iT® imaging system with a UV transilluminator with EtBr filter with 1.5 second exposure time.
Two-fold dilution series of ssRNA ladder (NEB) was mixed with EMBER500™ RNA Prestain Loading Dye, heated for 10 minutes at 70°C, then run on a non-denaturing 1% agarose/1X TBE gel. From left to right, loading amounts were 500, 250, 125, or 250 ng per lane. Samples in lanes 1-3 were mixed with loading dye at a ratio 1 volume of sample and 1 volume of dye, while the sample in lane 4 was mixed at a ratio of 1 volume of sample and 9 volumes of dye. The gel was imaged using a Thermo Fisher Safe Imager™ 2.0 Blue-Light Transilluminator in a UVP GelDoc-iT® darkroom with GelCam 310 2.0 camera (left, 0.5 second exposure time), or with a UV transilluminator and 535 nm filter for green dyes (center, 1 second exposure time) or EtBr filter (right, 2 second exposure time).
Total RNA was extracted from E. coli without DNase-treatment (lane 1) or with DNase-treatment (lane 2) to remove genomic DNA. RNA samples were prestained with EMBER500™ and then separated on a 1% agarose/1X TBE gel at 250 ng per lane. The position of the genomic DNA (gDNA) band and ribosomal RNA bands (23S, 16S, and 5S) are indicated to the left of the gel.

EMBER500™ RNA Prestain Loading Dye

EMBER500™ RNA Prestain Loading Dye provides single-step denaturing, staining, and loading of RNA, delivering brighter sensitivity than ethidium bromide on standard agarose gels.

EMBER500™ RNA Prestain Loading Dye

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SKU: 41032, 41032-250uL Categories: Tags: Brand:

Bright, Convenient RNA Prestain Loading Dye for Gel Electrophoresis

Evaluating RNA integrity is critical for gene expression and transcriptomics workflows, yet traditional staining methods like ethidium bromide (EtBr) offer limited sensitivity. EMBER500™ RNA Prestain Loading Dye streamlines RNA analysis with a single-step solution that denatures, stains, and loads RNA directly onto a regular agarose gel.

The result: faster, simpler, and far more sensitive detection compared to EtBr prestaining, without the need for complex and hazardous denaturing gels. EMBER500™ also stains DNA, allowing researchers to quickly identify genomic DNA contamination in RNA samples.

Compatible with both UV and blue LED gel imagers, EMBER500™ offers broad flexibility while avoiding UV safety hazards.

Key Features

  • Fast & convenient: Single-step denaturing, staining, and loading of RNA.
  • Superior sensitivity: Brighter, more sensitive than EtBr prestaining.
  • RNA integrity check: Enables detection of RNA quality and genomic DNA contamination.
  • Instrument compatibility: Works with UV or blue LED imagers and standard filters.
  • Safer workflow: Eliminates the need for hazardous denaturing gels.
  • Tracking dyes included: Bromophenol blue (~300 bp) and xylene cyanol (~3 kb) for electrophoresis monitoring.
Total RNA was extracted from E. coli without DNase-treatment (lane 1) or with DNase-treatment (lane 2) to remove genomic DNA. RNA samples were prestained with EMBER500™ and then separated on a 1% agarose/1X TBE gel at 250 ng per lane. The position of the genomic DNA (gDNA) band and ribosomal RNA bands (23S, 16S, and 5S) are indicated to the left of the gel.
Total RNA was extracted from E. coli without DNase-treatment (lane 1) or with DNase-treatment (lane 2) to remove genomic DNA. RNA samples were prestained with EMBER500™ and then separated on a 1% agarose/1X TBE gel at 250 ng per lane. The position of the genomic DNA (gDNA) band and ribosomal RNA bands (23S, 16S, and 5S) are indicated to the left of the gel.
Two-fold dilution series of ssRNA ladder (NEB) was mixed with EMBER500™ RNA Prestain Loading Dye, heated for 10 minutes at 70°C, then run on a non-denaturing 1% agarose/1X TBE gel. From left to right, loading amounts were 500, 250, 125, or 250 ng per lane. Samples in lanes 1-3 were mixed with loading dye at a ratio 1 volume of sample and 1 volume of dye, while the sample in lane 4 was mixed at a ratio of 1 volume of sample and 9 volumes of dye. The gel was imaged using a Thermo Fisher Safe Imager™ 2.0 Blue-Light Transilluminator in a UVP GelDoc-iT® darkroom with GelCam 310 2.0 camera (left, 0.5 second exposure time), or with a UV transilluminator and 535 nm filter for green dyes (center, 1 second exposure time) or EtBr filter (right, 2 second exposure time).
Two-fold dilution series of ssRNA ladder (NEB) was mixed with EMBER500™ RNA Prestain Loading Dye, heated for 10 minutes at 70°C, then run on a non-denaturing 1% agarose/1X TBE gel. From left to right, loading amounts were 500, 250, 125, or 250 ng per lane. Samples in lanes 1-3 were mixed with loading dye at a ratio 1 volume of sample and 1 volume of dye, while the sample in lane 4 was mixed at a ratio of 1 volume of sample and 9 volumes of dye. The gel was imaged using a Thermo Fisher Safe Imager™ 2.0 Blue-Light Transilluminator in a UVP GelDoc-iT® darkroom with GelCam 310 2.0 camera (left, 0.5 second exposure time), or with a UV transilluminator and 535 nm filter for green dyes (center, 1 second exposure time) or EtBr filter (right, 2 second exposure time).
Human total cellular RNA in water was mixed with EMBER500™ RNA Prestain Loading Dye or loading dye with 250 ug ethidium bromide (EtBr). Samples were heated for 10 minutes at 70°C, then run on a non-denaturing 1% agarose/1X TBE gel. From left to right, loading amounts were 200, 100, 50, or 25 ng/lane for total RNA. Lanes 1-4 used EMBER500™ RNA Prestain Loading Dye, lanes 5-8 used RNA loading dye with EtBr. The gel was imaged using a UVP GelDoc-iT® imaging system with a UV transilluminator with EtBr filter with 1.5 second exposure time.
Human total cellular RNA in water was mixed with EMBER500™ RNA Prestain Loading Dye or loading dye with 250 ug ethidium bromide (EtBr). Samples were heated for 10 minutes at 70°C, then run on a non-denaturing 1% agarose/1X TBE gel. From left to right, loading amounts were 200, 100, 50, or 25 ng/lane for total RNA. Lanes 1-4 used EMBER500™ RNA Prestain Loading Dye, lanes 5-8 used RNA loading dye with EtBr. The gel was imaged using a UVP GelDoc-iT® imaging system with a UV transilluminator with EtBr filter with 1.5 second exposure time.

Applications

  • Evaluation of total RNA integrity
  • Detection of DNA contamination in RNA preps
  • Gel electrophoresis of RNA for downstream workflows (qPCR, RNA-seq, IVT)
  • General RNA quality control in molecular biology labs

 

Notes

  • Not recommended for analysing low molecular weight RNA or ssDNA.
  • Not compatible with formaldehyde, glyoxal, or urea denaturing gels.
  • For maximum sensitivity (detecting as little as ≤5 ng RNA), use the EMBER™ Ultra RNA Gel Kit.

Format sizes:

  • 4 × 1 mL (Cat. #41032)
  • 250 µL (Cat. #41032-250uL)

✅ For research use only. Not for use in diagnostic procedures.

*Biotium Products are only available in Singapore and Thailand.

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