Traceable Fetal Bovine Serum: Biowest for Reproducible Cell Culture

Boost Research Consistency with Biowest FBS:

Frustrated with inconsistent cell culture results due to unreliable FBS? Biowest offers a premium solution! Our traceable, high-quality FBS empowers researchers to achieve reproducible cell growth and superior performance.

Does FBS Quality Really Matter? Absolutely!

Fetal Bovine Serum (FBS) is a vital yet often underestimated component of cell culture. While some researchers might use any available FBS, its quality significantly impacts your results. Inconsistent FBS can introduce unwanted variability, jeopardizing the reproducibility of your research. Inconsistent FBS quality can introduce hidden variables, leading to:

Unreliable Data: Variability in cell growth, function, and response to stimuli can skew your research findings.
Wasted Time & Resources: Inconclusive results lead to repeated experiments, costing valuable time and resources.
Difficulty Reproducing Results: Replicating experiments becomes challenging when a key factor (FBS quality) is uncontrolled.

Biowest FBS: The Solution for Reliable Cell Culture

Biowest FBS tackles these challenges by offering exceptional quality and traceability you can trust:

Unmatched Traceability: Trackable back to the source cow, ensuring consistent composition and performance across batches.
Enhanced Reproducibility: Minimize variability and achieve reliable results across your research, facilitating publication-quality data.
Superior Cell Growth: Supports optimal growth and function for diverse cell lines, allowing you to focus on your research goals.
Rigorously Tested: Passes strict cell culture tests (growth, plating efficiency, cloning efficiency) using established cell lines (HELA, L929, SP2/0-AG14, MRC-5).
Minimized Endotoxin: Lower endotoxin levels (≤10 EU/mL) for reduced cell stress and improved experimental outcomes.
Reduced Hemoglobin: Low hemoglobin content (≤25 mg/dL) minimizes interference with downstream assays.
Mycoplasma & Virus Free: Ensures a clean and safe cell culture environment.
Trusted by Leading Institutions: Widely used in top universities (NUS, CSI) and R&D labs across Southeast Asia.
ISO-Certified Manufacturing: Produced in facilities adhering to stringent ISO 9001:2008 and ISO 13485:2012 standards.

All our serum are sourced from BSE , FMD and BVD regions. Our very own Singapore Veterinary Authority has inspected all documentation and permits before the final product reaches your lab.

Cell Lines Tested

The following cell lines are tested with the serum:

HELA -Cancer Cell/Human.
L929 -Fibroblast-Mouse/ As Macrophage
SP2/0-AG14 -Mouse/Lymphoma
MRC- 5 -Human/Lung.

Cell Culture Tests: Cell Growth, Plating Efficiency, Cloning Efficiency.

We deliver Biowest solutions across South East Asia (Singapore, Thailand, Vietnam, Indonesia, Philippines, Myanmar) and Across China Only.

Reference Publications

Konstantopoulos G, Leventakou D, Saltiel DR, Zervoudi E, Logotheti E, Pettas S, Karagianni K, Daiou A, Hatzistergos KE, Dafou D, Arsenakis M, Kottaridi C.

HPV16 E6 Oncogene Contributes to Cancer Immune Evasion by Regulating PD-L1 Expression through a miR-143/HIF-1a Pathway. Viruses.

Viruses vol. 16,1 113. 12 Jan. 2024

doi:10.3390/v16010113

Article Snippet: “SiHa, CaSki and HEK293T cell lines were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA) and grown in Dulbecco’s Modified Eagle medium (DMEM, Biowest #L0103) supplemented with 10% fetal bovine serum (FBS, Biowest #S1810) (Gibco/Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA), 1% L-Glutamine (Biowest #X0550) and 1% Penicillin-Streptomycin (Biowest #L0022)…”

Mohamed, H. E., Asker, M. E., Kotb, N. S., & El Habab, A. M.

Human platelet lysate efficiency, stability, and optimal heparin concentration required in culture of mammalian cells

Blood research, 55(1), 35–43

DOI: https://doi.org/10.5045/br.2020.55.1.35

Article Snippet: “For HPL and FBS (#S1810-100, Biowest, Riverside, MO, USA) samples, the concentrations of IGF-1 and TGF-β1 were analyzed using enzyme-linked immunosorbent assay (ELISA) kits #IGF31-K01 (Eagle Biosciences, Inc., Amherst, NH, USA)”

Repossi R, Martín-Ramírez R, Gómez-Bernal F, Medina L, Fariña-Jerónimo H, González-Fernández R, Martín-Vasallo P, Plata-Bello J

Evaluation of Zonulin Expression and Its Potential Clinical Significance in Glioblastoma

Cancers. 2024; 16(2):356

DOI: https://doi.org/10.3390/cancers16020356

Article Snippet: “Dulbecco’s modified Eagle’s medium/Ham’s F-12 (DMEM/F12, Catalog No. 11330032; Gibco, Thermo Fisher Scientific, Waltham, MA, USA), containing L-Glutamine and 15 mM HEPES, supplemented with 10% fetal bovine serum (FBS, Catalog No. S1810; Biowest, Nuaillé, France), 59 mg/L Penicillin, 100 mg/L Streptomycin, and 25 mg/L Amphotericin (Catalog No. P3032, S9137 and A2411; Sigma-aldrich, San Luis, MO, USA)”

Lattmann, E., Lapaire, V., & Levesque, M. P.

Isolation and detection of extracellular vesicles from melanoma cells and liquid biopsies using size-exclusion chromatography and nano-flow cytometry

STAR protocols, 4(3), 102365

DOI: https://doi.org/10.1016/j.xpro.2023.102365

Article Snippet: “RPMI-1640 Sigma-Aldrich, USA R0883, Heat-inactivated fetal bovine serum (FBS) Biowest, France S1810, Exosome-depleted FBS, Gibco, USA A2720803, L-glutamine (200 mM) Gibco, USA 25030-24, Sodium pyruvate (100 mM) Sigma-Aldrich, USA S8636, Phosphate-buffered saline (PBS) Gibco, USA 14190144″
Dulbecco’s phosphate-buffered saline (DPBS) Gibco, USA 14190144
Sodium hydroxide (NaOH) pellets, 40 g/mol Merck, Germany 1310-73-2
PlasmoTest™ InvivoGen rep-pt1

Szymon Rutecki, Agnieszka Leśniewska-Bocianowska, Klaudia Chmielewska, Julia Matuszewska, Eryk Naumowicz, Paweł Uruski, Artur Radziemski, Justyna Mikuła-Pietrasik, Andrzej Tykarski, Krzysztof Książek

Serum starvation-based method of ovarian cancer cell dormancy induction and termination in vitro

Biology Methods and Protocols, Volume 8, Issue 1, 2023, bpad029

DOI: https://doi.org/10.1093/biomethods/bpad029

Article Snippet: “The A2780 and SKOV-3 cells were grown in RPMI-1640 medium (Biowest, Riverside, MO, USA, cat. no. L0501) with L-glutamine (2 mmol/l), penicillin (100 U/ml), streptomycin (100 g/ml) (Merck, cat. no. G6784), and 10% fetal bovine serum (FBS, Biowest, cat. no. S1810)”

Zhao, X., Chen, M. & Tan, J.

Knockdown of ZFR suppresses cell proliferation and invasion of human pancreatic cancer

Biol Res 49, 26

DOI: https://doi.org/10.1186/s40659-016-0086-3

Article Snippet: “Fetal bovine serum (FBS) was obtained from Biowest (Catalog Number: S1810, Loire Valley, France). The lentiviral expression vector (pFH-L) and packaging vectors (pVSVG-I and pCMVΔR8.92) were purchased from Hollybio (Shanghai, China)”

Testimonials

Biowest Traceable Fetal Bovine Serum (S1810-500) Used For Scratch Wound Assay

Wound healing assays (Scratch wound assay) are widely utilized in cancer research and studies of cell-cell interactions to investigate cell migration, particularly in the context of skin wound repair and cancer metastasis. In this method, a confluent monolayer of cells is mechanically scratched to create a “wound,” and the rate of closure is monitored over time.

In the experiment, HaCaT were routinely cultured Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS). All cells were cultured in a 37 degree Celsius, 5% carbon dioxide, humidified incubator. keratinocytes were routinely sub-cultured using 0.25 % trypsin and 1 mM Ethylenediaminetetraacetic acid. A linear scratch wound were created on a confluent layer of seeded cells and were treated in DMEM low glucose in the absence or presence of 10% FBS for 8 hours. Images were captured at regular intervals to analyse the wound closure over time.