Your Complete Guide for Seamless Fraction Collection with IZON TRPS Workflows

Researchers across Singapore and the region rely on the IZON AFC V2 to streamline fraction collection during TRPS measurements and qEV chromatography. To support your work from bench to bedside, we have curated a comprehensive hub that brings every AFC guide, video and troubleshooting step into one place. This page is designed to help you set up the AFC with confidence, resolve common issues quickly and achieve consistent, high quality EV fractions.

1. Quick Start Resources

Unboxing the AFC V2

Understand what comes in the box and how to prepare your system for first time use.
Watch video: Unboxing the AFC V2

Quick Start Guide

A concise printed guide to help you begin immediately.
Download: Automatic Fraction Collector V2 Quick Start Guide

User Manual

Access full specifications, maintenance instructions and complete system documentation.
Download: Automatic Fraction Collector V2 User Manual

2. Step by Step AFC V2 Setup

AFC Setup and Calibration

Learn how to assemble the AFC, connect it to your Exoid or qEV system and perform proper calibration for accurate collection.
Watch video: How to set up and calibrate the AFC

Setting the Nozzle Set

Guidance on correct nozzle selection and placement for reliable TRPS performance.
Watch video: How to set the nozzle set

Running a Sample on the AFC

A complete walk through of preparing your run, aligning the collection tubes and ensuring clean fraction separation.
Watch video: How to perform a run on the AFC

Changing the Nozzle

Learn how to safely replace the nozzle without compromising run quality.
Watch video: How to change the AFC V2 nozzle

3. Troubleshooting and Common Issues

AFC users often face repeatable challenges. We have summarised the most common issues and how you can resolve them quickly.

Vibration Detection Errors

If the AFC detects vibration and stops mid run, follow these steps to identify the source and restore stable operation.
Read guide: Troubleshoot vibration detection on the AFC V2

4. Frequently Asked Questions

1. Does the AFC V2 Automatic Fraction Collector require an external computer?
Answer: No. The AFC V2 is fully operated through its built in touch screen and does not require any external computer or laptop.

2. Are there components of the AFC V2 that need regular replacement or cleaning?
Answer: Yes. The main component to monitor is the nozzle tubing. If air bubbles or crystallised PBS salts accumulate inside, liquid flow will be blocked. We recommend removing the nozzle tubing after each day of use and flushing it thoroughly with DI water using a wash bottle. Allow it to air dry at room temperature before reattaching.
Since the valve presses against the nozzle tubing, slight deformation may occur. You can simply reshape it gently with your fingers. If you notice leakage or any damage, please contact us for assistance.

3. Which qEV columns are compatible with the AFC V2?
Answer: The AFC is compatible with all qEV columns except the qEV100

6. Additional EV Resources

Strengthen your EV workflow with these curated scientific guides.

• Step by Step EV and Exosome Workflow Every Researcher Should Know
• Why ExoBrite outperforms PKH and carbocyanine dyes
• Exoid TRPS versus traditional methods for EV characterisation
• Regulatory Roadmap for Exosome-Based Therapeutics: FDA, EMA & ASEAN Guide
• Efficient Extracellular Vesicles Isolation for Liquid Biopsy Analysis Using the Izon qEV Platform

These resources help you build a complete bench to bedside EV workflow.

7. Work with Atlantis Bioscience

We support EV researchers, imaging core facilities and translational teams across Singapore and Southeast Asia with:

• On-site AFC setup
• Hands on troubleshooting for Exoid and qEV
• Guidance on nozzle selection and nanopore sizing
• Technical support for TRPS data interpretation
• Imaging solutions for fluorescent EV labelling
• Consultation for translational EV development

Need help with your AFC workflow

Contact us for personalised support or to purchase qEV columns for your EV workflow.