Germ-free Testing Services GemPharmatech

Germ-free Testing Services GemPharmatech

GemPharmatech’s germ-free and gut microbiome service platform is a leading germ-free mouse production and experimental platform in China.

[vc_row][vc_column][vc_column_text]GemPharmatech’s germ-free and gut microbiome service platform is a leading germ-free mouse production and experimental platform in China. This platform owns 2000 m2 of facilities, hundreds of sterile isolators, reliable sterile feeding systems, and experimental technology. GemPharmatech is the first company that has been granted the Germ-Free Laboratory Animal Production License in Jiangsu Province and our germ-free mice production facilities have passed the on-site inspection by Jiangsu Province Science and Technology Department. We can provide worldwide scientific research institutions and pharmaceutical companies with services covering germ-free mouse production, breeding, sale and experiments.[/vc_column_text][vc_row_inner][vc_column_inner][vc_column_text]


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Customized Germ-free Services

[/vc_column_text][vc_column_text]GemPharmatech can supply a full set of proprietary germ-free housing apparatus and provide complete technical training to clients who are interested in establishing germ-free experimental platforms. We also provide services including customized sterility testing, aseptic instruments and mice feed.

Microbiota studies are important in a number of research fields, and gut microbiota plays a key role in the onset, progression, and treatment of metabolic diseases, inflammatory bowel disease, tumor immunotherapy, and neurological disorders. Our germ-free mice and gut microbiome service platform can be used to explore the gut microbiome according to the client demands.[/vc_column_text][vc_column_text]

Microbiology Services In Vivo Services In vitro Analysis Bioinformatics
·Microbial Culture


·Fecal Microbiota extraction


·Single Strain/Flora Transplantation


·Bacterial Identification:

Qualitative testing

Quantitative testing

·Drug Delivery:

Intraperitoneal, subcutaneous, oral gavage, intravenous.


·Blood Collection:

Submandibular plexus, tail vein, retro-orbital.



Body weight, blood glucose, food intake.


·Sample Collection:

Blood, feces, urine.

·Cell Experiments:

Cell isolation and culture

Tumor cell line inoculation


·Flow Cytometry:

 Cell analysis

Antibody binding assay


·Pathology Experiments:

Staining and sectioning



·Molecular Assays:


Blood Biochemistry


·16s rRNA Amplicon Sequencing:

OTU/ASV taxonomic analysis

Community composition analysis

Alpha diversity analysis

Beta diversity analysis


·Metagenomic Sequencing:

Species annotation and compositional profiling

Gene prediction and functional annotation

Alpha and Beta diversity analysis

Key biomarker screening

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Evaluation of Anti-tumor Effect of Gut Microbiota

[/vc_column_text][vc_column_text]More and more studies have shown that gut microbiota have notable impact on cancer therapy. Therapies such as chemo-therapeutic drugs and immune checkpoint inhibitors can affect the integrity of the intestinal barrier and influence the microbe-host interaction. Gut microbiota interacting with the matrix, nerve, endocrine, immune cells in the intestinal tract can have systemic effects on hosts, and ultimately affect the host’s immune response, making gut microbiota essential for anti-tumor efficacy studies. Using severely immunodeficient germ-free NCG mouse models, we established a triple humanized mouse models which have human tumor cells, human immune checkpoint components, and human gut microbiota, allowing for evaluation of the impact of gut microbiota on the anti-tumor efficacy of anti-tumor drugs.[/vc_column_text][vc_column_text][/vc_column_text][/vc_tta_section][vc_tta_section title=”Microbiota Extraction and Transplantation” tab_id=”1677453757880-5df23342-c421″][vc_column_text css=”.vc_custom_1677454977058{padding-top: 60px !important;padding-bottom: 60px !important;background-image: url( !important;}”]

Microbiota Extraction and Transplantation

[/vc_column_text][vc_column_text]GemPharmatech has several Luciferase-modified cell lines, comprising solid and hematological malignancies, which provide a wealth of tumor models for evaluating efficacy of new cancer therapies. What’s special about these lines? Cell lines that are modified to be tagged with Luciferase allows imaging. That means, as cancer cells metastases/develop across the body, it can be imaged because the luciferase-tag makes it glow under a special machine.[/vc_column_text][vc_column_text]




Advanced BACRTON anaerobic chambers are used for microbial isolation protecting intestinal anaerobic strains from external disturbances (e.g., oxygen).

A well-designed special cryoprotectant is used to maintain the activity of microbiota.

A well-established Tecniplast ISOcage + IBS system can enable higher throughput experiments compared with traditional isolators.

Comparing the donor and recipient microbiota can determine the transplantation efficiency.

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Germ-free Rederivation and Breeding

[/vc_column_text][vc_column_text]Given the presence of microbiota in SPF mice, it is hard to study the microbe-host interaction. To overcome this challenge, GemPharmatech has become the first to establish the germ-free embryo transfer technique in China. Nearly 20,000 germ-free strains can be obtained by sourcing KO or CKO strains from the GemPharmatech Knock Out All Project mouse strain library through our germ-free rederivation and breeding services.


  • Advanced purification technique: Our germ-free embryo transfer technique can eliminate microbial contamination and enhance purification.
  • Experienced technical team: Our yearly capacity is nearly 100 germ-free strains; a single batch of germ-free rederived progeny can meet the needs of multiple research projects.
  • Strict sterile environment: We have first-class sterile breeding facilities and strain management practices.
  • High success rate: Up to date, we have a 100% success rate in germ-free rederivation.

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Microbial Strain Culture and Colonization

[/vc_column_text][vc_column_text]Colonization of single microbial strain or flora in germ-free mice is important for investigation of colonization resistance, microbial metabolism, and microbe-host interaction. It has also been widely used in studying the function of microbial strains, health benefits of probiotics, and effects of engineered bacteria.. GemPharmatech has established a comprehensive platform for microbial culture and colonization which includes in vitro culture, in vivo colonization, phenotyping, and sequencing analysis.


  • A well-equipped microbial platform with advanced BACRTON anaerobic chambers to better simulate the intestinal environment.
  • A well-developed strain transplantation method that can successfully colonize the oral cavity, gastrointestinal tract, reproductive tract, and respiratory tract.
  • A well-established QC system with no contamination that ensures the authenticity, accuracy and reliability of the experimental results.
  • A well-established service platform that provides services in isolation, identification, revival, enrichment, cryopreservation, and physiological and biochemical examination.

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