Antibody Discovery Services

We provide a broad range of DNA technologies for antibody services. Our technical platforms span all stages of antibody discovery by integrating antibody genes sequence, de novo antibody design, antibody humanization, antibody genes synthesis, recombinant antibody expression, monoclonal antibody preparation and polyclonal antibody preparation. We aim to use our high-quality products and cost-effective solutions to support the development of biobased medicines within academia, biotechnology, and pharmaceutical industries.

What we can do for antibody related services

Our Antibody Discovery Services Case Studies

Algorithm guided antibody design of an antibody

Here we used a computational method to aid the de novo design of PXX antibody: The PXX structure was first modeled using a homology-based modeling method. We extracted the frameworks and complementary determinant regions (CDRs) from antibody structures. Using frameworks as templates, we assembled CDRs onto the frameworks to construct an antibody library, and the antibody structures were modeled from their homologous protein structures. Then, the antibodies were docked onto the antigen and ranked according to the interaction energy. A certain number of best antibody hits were selected and the antibody sequences were synthesized and screened to get potential antibodies.

PXX is an important negative immunoregulatory factor that was expressed by tumor cells and reduces the body’s immune response. Antibodies that bind to PXX and block its signaling pathway can be used as therapeutics for the treatment of carcinoma. In this case, our designed PXX antibody achieved an affinity of 10^-11 M, which provided a novel anti-PXX antibody and candidate drugs. The time period and cost for antibody discovery were greatly reduced using this computational designing method.

Antibody library construction for a target protein

For protein CXX, we implemented a novel approach to construct antibody library:  We extracted the frameworks and complementary determinant regions (CDRs) were extract from antibody structures and sequences. Using frameworks as templates, we assembled CDRs onto the frameworks to construct an antibody library, and the antibody structures were modeled from their homologous protein structures. The antibodies were docked onto the CXX protein and ranked according to the interaction energy. We then constructed an antibody library with around 10⁶ antibody sequences according to the results. These sequences were synthesized and screened. The functionally active molecules were then obtained for further research.

Antibody discovery through bioinformatics analysis for Z virus infection

Blood samples were collected on the 6^th and 13^th day after Z virus infection. The antibody repertoire was sequenced. The NGS data was analyzed and clustered to get germline and CDR information. By comparing the results from two samples, we were able to select the top 50 differentially expressed antibodies. Then, the antibody genes were synthesized, expressed, and characterized in HEK cells. The active antibody to Z virus was screened using immune repertoire sequencing method.