Wheat Germ Agglutinin (WGA) Conjugates

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Biotium products are distributed only in Singapore and Thailand.

A wheat germ agglutinin (WGA) lectin labeled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine and stains yeast bud scars as well as the cell membrane of gram+ bacteria and mammalian cells.

PRODUCT ATTRIBUTES

Cellular localization	Membrane/cell surface
For live or fixed cells	For fixed cells, For live/intact cells
Cell permeability	Membrane impermeant
Fixation options	Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)
Colors	Blue, Green, Orange, Red, Far-red, Near-infrared

PRODUCT DESCRIPTION

Wheat germ agglutinin (WGA) is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram+ bacteria and mammalian cells.

Stain yeast bud scars as well as the cell membrane of gram+ bacteria and mammalian cells or tissues
Suitable for Western Blot, ELISA, immunohistochemistry and other standard immunoassay applications
A retrograde or anterograde neuronal tracer
Withstands fixation and permeabilization
Choice of 13 CF® dyes from UV to near-infrared or HRP
Superior CF® dyes are bright, photostable, and water-soluble

WGA is commonly used to label glycoproteins for imaging of the plasma membrane in live or fixed cells, for staining of tissue sections, or for western blotting. WGA can be used as a gram stain to fluorescently label gram+ bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae.

Find the Right Stain for Your Application

WGA and other lectins are carbohydrate binding proteins that recognize specific sugar moieties on glycoproteins. The presence and distribution of these targets vary between cell types and tissues. As a result, other cell surface stains or other lectin conjugates, Concanavalin A (Con A) Conjugates and PNA Lectin Conjugates, may produce better surface staining and may be more appropriate for your cell type. Lectin conjugates can be used to selectively stain the cell surface of live cells, and withstand fixation and permeabilization. When cells are fixed and permeabilized before staining, fluorescent lectins stain both cell surface and organelles in the secretory pathway. Lectins may be toxic or stimulatory to live cells depending on cell type. To find the right stain for your application, see our Membrane & Cell Surface Stains Comparison, or download our Membrane & Surface Stains Brochure. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Wheat Germ Agglutinin Conjugates

Product	Conjugation	Ex/Em	Size	Catalog No.	Purchase
HRP WGA	HRP	N/A	1 mg	29073	Purchase 29073
CF®350 WGA	CF®350	347/448 nm	1 mg	29021-1	Purchase 29021-1
CF®350 WGA	CF®350	347/448 nm	5 mg	29021	Purchase 29021
CF®405S WGA	CF®405S	404/431 nm	1 mg	29027-1	Purchase 29027-1
CF®405S WGA	CF®405S	404/431 nm	5 mg	29027	Purchase 29027
CF®405M WGA	CF®405M	408/452 nm	1 mg	29028-1	Purchase 29028-1
CF®405M WGA	CF®405M	408/452 nm	5 mg	29028	Purchase 29028
CF®488A WGA	CF®488A	490/515 nm	1 mg	29022-1	Purchase 29022-1
CF®488A WGA	CF®488A	490/515 nm	5 mg	29022	Purchase 29022
CF®532 WGA	CF®532	527/558 nm	1 mg	29064-1	Purchase 29064-1
CF®532 WGA	CF®532	527/558 nm	5 mg	29064	Purchase 29064
CF®555 WGA	CF®555	555/565 nm	5 mg	29076	Purchase 29076
CF®555 WGA	CF®555	555/565 nm	1 mg	29076-1	Purchase 29076-1
CF®568 WGA	CF®568	562/583 nm	5 mg	29077	Purchase 29077
CF®568 WGA	CF®568	562/583 nm	1 mg	29077-1	Purchase 29077-1
CF®594 WGA	CF®594	593/614 nm	1 mg	29023-1	Purchase 29023-1
CF®594 WGA	CF®594	593/614 nm	5 mg	29023	Purchase 29023
CF®633 WGA	CF®633	630/650 nm	1 mg	29024-1	Purchase 29024-1
CF®633 WGA	CF®633	630/650 nm	5 mg	29024	Purchase 29024
CF®640R WGA	CF®640R	642/662 nm	1 mg	29026-1	Purchase 29026-1
CF®640R WGA	CF®640R	642/662 nm	5 mg	29026	Purchase 29026
CF®680 WGA	CF®680	681/698 nm	1 mg	29029-1	Purchase 29029-1
CF®680 WGA	CF®680	681/698 nm	5 mg	29029	Purchase 29029
CF®680R WGA	CF®680R	680/701 nm	1 mg	29025-1	Purchase 29025-1
CF®680R WGA	CF®680R	680/701 nm	5 mg	29025	Purchase 29025
CF®770 WGA	CF®770	770/797 nm	1 mg	29059-1	Purchase 29059-1
CF®770 WGA	CF®770	770/797 nm	5 mg	29059	Purchase 29059

Wheat Germ Agglutinin (WGA) Conjugates