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Tyramide Amplification Buffer Plus
Tyramide Amplification Buffer Plus
Tyramide Amplification Buffer Plus is an enhanced TSA buffer delivering improved brightness, specificity, and sensitivity for immunostaining in cells and tissue sections.
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Optimised amplification buffer for brighter, more sensitive tyramide signal amplification in cells and tissues
Tyramide Amplification Buffer Plus is an optimised reagent developed to maximise the performance of tyramide signal amplification (TSA) in immunostaining workflows. Compared to the original Ready-to-Use Tyramide Amplification Buffer (Cat. No. 22027), this improved formulation delivers significantly enhanced signal brightness, specificity, and sensitivity.
The buffer is designed for use in cell and tissue staining applications, including IHC, ICC, and other TSA-based fluorescence assays. It is fully compatible with CF® Dye tyramides, TyraMax™ Amplification Dyes, and other commonly used tyramide reagents, making it a flexible choice for both established and high-plex staining workflows.
Key Features
- Designed for tyramide signal amplification in cell and tissue immunostaining
- Significantly improved brightness, specificity, and sensitivity compared to the original Ready-to-Use buffer
- Compatible with CF® Dye tyramides, TyraMax™ dyes, and other TSA reagents
- Suitable for both manual and automated staining workflows
- Available in 250 or 1000 reaction sizes
* Based on a 100 µL reaction volume. Actual number of reactions may vary depending on specimen size and staining setup.
Components
- 1X Tyramide Amplification Buffer Plus
- 30% Hydrogen Peroxide
Applications
- Immunohistochemistry (IHC)
- Immunocytochemistry (ICC)
- TSA-based multiplex immunofluorescence
- Spatial biology and high-sensitivity target detection
Tyramide Amplification Buffer Plus is supplied as a standalone reagent and is also included as a core component in Biotium’s Tyramide Amplification Kits. For optimal performance in high-plex and spatial biology applications, it is recommended for use with TyraMax™ Amplification Dyes.
Note: Biotium product is only available in Singapore, Thailand and Malaysia.
Variations
| Pack Size | Number of Reactions | Catalog Number |
|---|---|---|
| Trial Size | 250 reactions | 22029-T |
| Full Size | 1000 reactions | 22029 |
* Based on a 100 µL reaction volume. Actual number of reactions may vary depending on sample and specimen size.
Publications
Intratumoral bacteria are immunosuppressive and promote immunotherapy resistance in head and neck squamous cell carcinoma
Natalie L. Silver, Jin Dai, et al., Daniel J. McGrail (2026)
Nature Cancer | IF: 28.5
” … 2% BSA in PBS-T for antibody staining or moved directly to in situ hybridization.. For antibody staining, following primary and secondary antibody incubations (Supplementary Table 5), tyramide signal amplification was performed with CF550R dye (Biotium, 96077) in amplification buffer (0.1 M borate pH 8.5 with 0.003% hydrogen peroxide).. Bacterial FISH was performed as described previously81 using the pan-bacterial 16S rRNA probe EUB338 … “
Oligodendrocyte death initiates synchronous remyelination to restore cortical myelin patterns in mice
Timothy W. Chapman, Genaro E. Olveda, et al., Robert A. Hill (2023)
Nature Neuroscience | IF: 20.0
“In the study, Tyramide Amplification Buffer Plus from Biotium was employed during the immunostaining process to enhance the signal for GPR17, facilitating effective visualization of br… “
OSMRβ-mediated signals on resident fibroblasts restore healing in diabetic skin wounds through promoting angiogenesis and granulation tissue formation
Yuko Ishida, Yumi Kuninaka, et al., Toshikazu Kondo (2025)
Communications Biology | IF: 5.2Â
“After the incubation the sections with HRP-conjugated goat anti-rabbit IgG, immune complexes were visualized using Tyramide Amplification Buffer Plus (22029, Biotium, Fremont, CA) with Tyramide Signal Amplification Kits (33020, Biotium-XX, Biotium) and LSAB2 Streptavidin-HRP (K1016, Dako, Carpinteria, CA) according to the manufacturer’s instructions.. “
Adipose tissue-derived fibroblasts engage in immune–stromal crosstalk during obesity-aggravated atherosclerosis in mice
Mikkola Lea, Bölük Aydin, et al., Lönnberg Tapio | bioRxiv (Preprint) | 2025
“… antibody diluent (10 minutes at RT) or Dako REAL antibody diluent (for PI16), primary antibody incubation (20 minutes at RT, Supplementary Table 1), secondary antibody incubation (10 minutes at RT, Supplementary Table 1), tyramide signal amplification by incubating slides with corresponding fluorophore-conjugated tyramide (Supplementary Table 1) for 10 minutes in 2 nM final concentration in Tyramide Amplification Buffer Plus (15129, Biotium), supplemented with fresh 0.0015% hydrogen peroxide.”
LSD1 serine 166 is a phosphorylation switch for chromatin landscaping, gene activation, and tissue remodeling
Sharma Mukund, Aakula Anna, et al., Westermarck Jukka | bioRxiv (Preprint) | 2025
“… a polymeric HRP-conjugated secondary antibody (DPVR110HRP-IL, Immunologic) for 10 min.. Slides were incubated for 10 min with appropriate fluorophore-conjugated tyramide (CF-dye tyramides, Biotium, CA, USA) in 2 nM final concentration in Tyramide Amplification Buffer Plus (Biotium), supplemented with fresh 0.0015% hydrogen peroxide immediately prior to use.. Slides were subjected to HIER in BOND Epitope Retrieval Solution.”
