SynaptoGreen™ C4 (Equivalent to FM®1-43)
SynaptoGreen™ C4 (Equivalent to FM®1-43)
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A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors.
PRODUCT ATTRIBUTES
| CAS number | 149838-22-2 |
|---|---|
| Cellular localization | Membrane/vesicular |
| For live or fixed cells | For live/intact cells |
| Assay type/options | Real-time imaging |
| Colors | Green |
| Excitation/Emission | 480/598 nm (in membranes) |
PRODUCT DESCRIPTION
A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors. Research results from the Tsien and Kay labs showed that brain slices can be stained with SynaptoGreen™ when SR101 (Sulforhodamine 101, 80101) or ADVASEP-7 (70029) is used to reduce the background staining. Biotium offers several Nerve Terminal Staining Kits for staining brain slices. Also see AM1-43 (70024), which is similar to SynaptoGreen™, but with a formaldehyde-fixable amine group.
SynaptoGreen™ also can be used to stain bacteria (gram-positive or gram-negative) and yeast, although in yeast it rapidly internalizes to vacuolar membranes. See our Cellular Stains Table for more information on how our dyes stain various organisms.
- λEx/λEm: 510/625 nm (in MeOH); 480/598 nm (in membranes)
- Red solid soluble in water
- C30H49Br2N3
- MW: 612
[accordions]
[accordion title=”Protocols”] PI-SynaptoGreen SynaptoRed,[/accordion]
[accordion title=”SDS”] sds-70020,
sds-70022[/accordion]
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