PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (nuc) – 200 assays
PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (nuc) – 200 assays
$359.00
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Product Description
A kit designed to perform viability PCR on Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the nuc gene.
Product Description
This kit is designed to perform viability PCR on Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the nuc gene.
Features
- Selectively detect viable Staphylococcus aureus using qPCR
- Contains primers to amplify the nuc gene
- Works even in complex samples or mixed cultures
- Sufficient reagents to treat 80 bacterial cultures with PMA
- Sufficient reagents for 200 PCR reactions
Kit components
- PMA dye
- Forget-Me-Not™ EvaGreen® qPCR Master Mix
- ROX reference dye
- nuc primer mix, 5 uM
About this kit
Staphylococcus aureus is an anaerobic gram positive bacterium that can cause of a variety of infections in humans, and methicillin-resistant S. aureus is a rising cause of hospital-acquired infections. The primers included in this kit have been tested for amplification of the S. aureus-specific gene nuc gene from genomic DNA from both methicillin-susceptible and methicillin-resistant strains of S. aureus and have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix. Biotium also offers the Staphylococcus aureus (mecA) PMA-PCR kit, which includes primers against the methicillin-resistance gene mecA.
To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.
Strain-Specific Bacterial Viability PCR Kits
| Bacteria Strain | Gene name | Kit with PMA (catalog #) | Kit with PMAxx™ (catalog #) |
|---|---|---|---|
| Salmonella enterica | invA | 31033 | 31033-X |
| Mycobacterium tuberculosis | groEL2 | 31034 | N/A |
| Staphylococcus aureus | nuc | 31035 | N/A |
| MRSA | mecA | 31036 | N/A |
| E. coli O157:H7 | Z3276 | 31037 | 31037-X |
| E. coli | uidA | 31050 | 31050-X |
| Listeria monocytogenes | hly | 31051 | 31051-X |
| Legionella pneumophila | mip | 31053 | N/A |
Don’t see your favorite strain? Let us know at [email protected].
PMAxx technology is covered by granted and/or pending US and international patents.
References
Find a list of PMA and PMAxx™ references and a list of validated bacterial strains under Supporting Documents.
Product Attributes
| Viability dye | PMA |
|---|---|
| Storage Conditions | Store at -10 to -35 °C |
Protocols
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