Bovine Serum Albumin (BSA) Lyophilised pH~7

Bovine Serum Albumin (BSA) Lyophilised pH~7

Bovine Serum Albumin (BSA) Lyophilised pH~7

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Bovine Serum Albumin

  • Helps to stabilize enzyme and proteins during purification or DNA digestion.
  • Reduce adhesion of protein of interest or enzymes to the reaction tube surface, walls or pipette tips.
  • Used as a blocking agent in DNA and protein labelling experiments to minimise background.

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[accordion title=”Packaging”] Available in 100, 500, 1kg [/accordion]

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– Our standard grade, lyophilized flakes (suitable for cell culture and molecular biology)
– Dissolves quickly
– Protease free fatty acid free
– BSE free and sourced from New Zealand
– Widely accepted in key institutions like NUS, CSI (Singapore) and various R&D laboratories across S.E.A.
– All products are produced in ISO 9001:2008 and ISO 13485:2012 facilities

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[accordion title=”Methods for Preparation”]

Primary Purification methods
– Ethanol Fractionation Methods

Additional purification steps:
– Heat Shock

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[accordion title=”Application”]

Bovine Serum Albumin (also known as BSA or Fraction V) is commonly used as a protein concentration standard in laboratory experiments. Our BSA is suitable for numerous biochemical applications include ELISA, immunoblots and immunochemistry. Our customers have also used it as a nutrient or carrier protein for cellular and microbial work.

Suitable for:
– Cell culture
– Molecular biology
– Molecular weight Markers and protein standards

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[accordion title=”COA”]N/A[/accordion]
[accordion title=”MSDS/Tech Sheet”] p6154_msds.pdf

p6154_techsheet.pdf[/accordion]
[accordion title=”Product Brochure”] N/A [/accordion]
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Reference Publications

Younis, F. A., Saleh, S. R., El-Rahman, S. S. A., Newairy, A. A., El-Demellawy, M. A., & Ghareeb, D. A.

Preparation, physicochemical characterization, and bioactivity evaluation of berberine-entrapped albumin nanoparticles

Scientific reports12(1), 17431

DOI: https://doi.org/10.1038/s41598-022-21568-8

Article Snippet:Bovine serum albumin (BSA) lyophilized form (P6154-10GR, pH ~ 7, Mw 66 KDa) supplied from Biowest (France). The activated partial thromboplastin time (APTT) kit was purchased from Biomed Diagnostics Co., Ltd. (Egypt).”

 

Casonato Melo, C., Fux, A. C., Himly, M., Bastús, N. G., Schlahsa, L., Siewert, C., Puntes, V., Duschl, A., Gessner, I., & Fauerbach, J. A.

Recovering What Matters: High Protein Recovery after Endotoxin Removal from LPS-Contaminated Formulations Using Novel Anti-Lipid A Antibody Microparticle Conjugates

International journal of molecular sciences, 24(18), 13971

DOI: https://doi.org/10.3390/ijms241813971

Article Snippet: “Donkey anti-goat-phycoerythrin antibody (anti-Goat-PE, 705-115-147) and goat anti-human-phycoerythrin (anti-Human-PE, 109-116-098) were purchased from Jackson Immuno Research. Bovine serum albumin (BSA, P6154-500GR) was purchased from BioWest. Pluronic F-68 (A1288,0100) was purchased from PanReac AppliChem.”

 

Koivunotko E, Snirvi J, Merivaara A, Harjumäki R, Rautiainen S, Kelloniemi M, Kuismanen K, Miettinen S, Yliperttula M, Koivuniemi R.

Angiogenic Potential of Human Adipose-Derived Mesenchymal Stromal Cells in Nanofibrillated Cellulose Hydrogel

Biomedicines. 2022; 10(10):2584

DOI: https://doi.org/10.3390/biomedicines10102584

Article Snippet: “After 20 min, the cells were blocked with PBS-T containing 3% (m/v) bovine serum albumin (BSA, Cat. #P6154; Biowest, France) and 0.3 M glycine (Cat. #33226; Sigma-Aldrich) for one hour at room temperature”

 

Karim, F., Riou, C., Bernstein, M. et al.

Clearance of persistent SARS-CoV-2 associates with increased neutralizing antibodies in advanced HIV disease post-ART initiation

Nat Commun 15, 2360 (2024)

DOI: https://doi.org/10.1038/s41467-024-46673-2

Article Snippet: “Foci were stained with a rabbit anti-spike monoclonal antibody (BS-R2B12, GenScript A02058) at 0.5 μg/mL in a permeabilization buffer containing 0.1% saponin (Sigma-Aldrich, S7900), 0.1% bovine serum albumin (BSA, Biowest, P6154) and 0.05% Tween-20 (Sigma-Aldrich, P9416) in PBS”

 

Ileana Conde-Rodríguez, Guadalupe Delgado-López, Erick Armenta-Castro, Ivonne Ramírez-Díaz, Maricruz Anaya-Ruiz, et al.

Evaluation of Serum Levels and Expression of Galectin-4 in Cervical Cancer

BioMed Research International, vol. 2020, Article ID 6756723, 9 pages, 2020

DOI: https://doi.org/10.1155/2020/6756723

Article Snippet: “Afterward, the tissues were incubated for 1 hour with blocking buffer containing 5% bovine serum albumin (P6154, Biowest, Riverside, MO, US.) in 1x PBS”

 

Mendieta-Carmona V, Delgado-López G, Reyes-Leyva J, Gutiérrez-Quiroz CT, Vazquez-Zamora VJ, et al.

Galectin-9 Expression is Correlated to Cervical Squamous Cell Carcinoma Progression and Overall Survival

Onco Targets Ther. 2023;16:891-904

DOI: https://doi.org/10.2147/OTT.S433710

Article Snippet: “Next, the tissues were incubated with blocking buffer containing 5% bovine serum albumin (P6154, Biowest, Riverside, MO, US) in 1× PBS for 1 hour”

Documents

Scientific Reports

International Journal

Biomedicines

Nature Communication

Research Article

Original Research