OMEGAACE-4mm Triple-Chamber Neuronal Co-Culture

OMEGAACE-4mm Triple-Chamber Neuronal Co-Culture


Triple-Chamber Neuronal Co-Culture


Let your neurons do what they were meant to do – make synaptic connections! Get even more out of your neuronal cultures and co-cultures with the latest in microfluidic devices from eNUVIO.

OMEGAACE-4mm devices can be used to set up in vitro triple co-culture to study neuronal propagation

OMEGAACE-4mm device features:

  • Ready-to-use: sterile, hydrophilic, and pre-bonded to a high-transmissive glass coverslip
  • Open-top chamber design provides full access to all cultures – use explants or spheroids in your experiments!
  • Choose between Starter Kit and Refill Kit

Do you need a narrower middle chamber? Then the OMEGAACE or OMEGAACE-2mm might be for you. And did you already check out our HTS compatible OMEGA96-ACE microplate?


The OMEGA series of microfluidic devices from eNUVIO are perfectly suited for experiments involving neuronal co-culture and compartmentalization. Each of the growth chambers of the device are physically separated by a series of microfluidic channels that serve to fluidically isolate each of the adjoined chambers. These microchannels are large enough however, to allow for neurites/axons to project into and interact with cultures located in the adjacent chamber. OMEGAACE-4mm devices each contain three serially-adjoined culture chambers. Compared to our two-chamber devices, triple-chamber devices provide researchers with the ability to perform more specialized co-culture and compartmentalization experiments.

The unique open-chamber design of OMEGA series devices gives scientists direct access to their cultures, enabling researchers to explore new types of experimental paradigms not possible with standard closed-chamber devices.  For example, the chambers are large enough to house tissues that are several millimeters in diameter, accommodating tissue explants or self-assembled 3D cultures (e.g. spheroids, organoids). Additionally, conventional patch-clamp electrodes can openly access the cultures, allowing researchers to examine the electrophysiological properties of the culture.

OMEGA devices are shipped out ready to use. They are sterile, hydrophilic and are pre-bonded to high-transmissive #1.5 glass coverslips. Simply coat the growth chambers and plate your cells – it’s that easy. Maintaining healthy cultures over a long period of time is even easier with OMEGA devices, owing to the evaporation-minimizing inserts included in every kit. Not only do these simple reusable culture inserts contain a built-in circular well that reduces the evaporation rate from the device’s chambers when filled with fluid, they also serve to physically stabilize the device for secure transportation of the device within the culture room.

OMEGAACE-4mm devices can be used for in vitro neuronal propagation studies.

Culture compatibility:

  • iPSC-derived cultures
  • Self-assembled 3D cultures (e.g. spheroids, organoids)
  • Primary cultures
  • Explant tissues
  • Immortalized cell lines

OMEGAACE-4mm specifications:

  • Glass coverslip: 22 mm, round, #1.5 thickness
  • Chamber #2 channel width: 4 mm
  • Chamber #1 and #3 width: 4.5mm
  • All chambers are fully accessible
  • Number of microchannels per interface: 70
  • Number of interfaces per device: 2
  • Microchannel length: >630 µm

The OMEGAACE-4mm Starter Kit includes:

  • 4 x OMEGAACE-4mm devices (8 experiments)
  • 4 x evaporation minimizing culture inserts (for 35 mm dishes)
  • 4 x sterile culture dishes (35 mm, round)
  • 1 x microscopy adapter for OMEGA series devices

The OMEGAACE-4mm Refill Kit includes:

  • 4 x OMEGAACE-4mm devices
  • 4 x sterile culture dishes (35 mm, round)

OMEGAACE-4mm Triple-Chamber Neuronal Co-CultureOMEGAACE-4mm Triple-Chamber Neuronal Co-Culture