These kits allow optimal labeling of single-chain nanobodies with CF® dyes or biotin in 30 minutes without a purification step. The labeling tolerates common buffer components including BSA.

Mix-n-Stain™ Nanobody Labeling Kits are designed specifically for labeling single-domain Nanobodies® (also called camelid single variable or VHH domains) with one of Biotium’s bright and photostable CF® dyes or biotin. The kits allow optimal labeling of between 5-50 ug of nanobody in just 30 minutes, with minimal hands-on time and no purification. Labeling tolerates common buffer additives, including BSA.

The kits come with a selection of our next-generation CF® dyes or biotin. CF® dyes have advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes (learn more about CF® Dyes).

Features

• Label 5-20 ug or 20-50 ug of Nanobody®
• Choice of 7 CF® Dye colors or biotin
• Simple 30 minute labeling, no purification
• Compatible with common stabilizers
• Dye options for super-resolution and 2-photon imaging (learn more)

Choosing the Right Kit for Your Application

Before selecting a Mix-n-Stain™ Nanobody Labeling Kit, please download the Product Protocol to see our Kit Compatibility and Protocol Selection guidelines.

For labeling IgG antibodies, please use one of our Mix-n-Stain™ Antibody Labeling Kits. We also offer Mix-n-Stain™ Small Ligand Labeling Kits, for labeling SNAP-tag®, CLIP-tag™, and HaloTag® ligands, or other small molecules. See Mix-n-Stain™ Product Lines below for our full selection of antibody, protein, and ligand labeling kits.

Mix-n-Stain™ Nanobody Labeling Kits

Note: Before selecting a kit, please download the Product Protocol to see Kit Compatibility and Protocol Selection guidelines.