KBM501 Activation Media for Human NK Cells
KBM501 Activation Media for Human NK Cells
$565.00
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KBM 501: Premium NK Cell Activation Media for Advanced Cell Therapy Research
Maximize Human NK Cell Activation for Potent Immunotherapy Applications
For researchers driving innovation in NK cell therapy, KBM 501 delivers unparalleled activation of human NK cells. This specialized media is engineered to prime NK cells for robust expansion and potent cytotoxic activity, essential for cutting-edge immunotherapy research.
Key Benefit of KBM 501:
- Targeted NK Cell Activation: Specifically designed to trigger optimal activation of human lymphocytes like NK cells, setting the stage for effective cell therapy development.
- High-Potency IL-2: Fortified with 2810 IU/mL of recombinant human IL-2, ensuring powerful and consistent NK cell stimulation.
- Defined, GMP-Compatible Formulation: Composed of human serum albumin, transferrin, insulin, and IL-2, aligning with rigorous regulatory standards for regenerative medicine.
- Enhanced pH Stability: Advanced buffering capacity minimizes pH fluctuations, maintaining an ideal environment for sensitive NK cell activation.
- Regulatory Confidence: Backed by an FDA Drug Master File registration and acknowledgement for regenerative medical products.
Applications of KBM 501:
- NK Cell Therapy Development: Ideal for activating NK cells prior to expansion, crucial for adoptive cell therapy targeting cancer and viral infections.
- Immunotherapy/Immunological Research: Enables precise studies of NK cell activation pathways and functional responses.
- Drug Discovery: Facilitates the screening of therapeutic agents that modulate NK cell activity.
Why KBM 501 is the Optimal Choice for NK Cell Activation:
- Consistent, Reproducible Results: A defined formulation ensures reliable and predictable NK cell activation.
- Seamless Integration with Expansion Protocols: Designed for use with KBM 502 and KBM 502B for a complete NK cell therapy workflow.
- Streamlined Research: Ready-to-use format simplifies the activation process, saving valuable time and resources.
- Regulatory-Ready: Supports your journey from research to clinical translation with qualified raw materials.
Elevate your NK cell therapy research with KBM 501. Contact us to request a quote and discover how our activation media can empower your next breakthrough.
Documents
Publications
Nagai, Kazuhiro et al. “Highly Activated Ex Vivo-expanded Natural Killer Cells in Patients With Solid Tumors in a Phase I/IIa Clinical Study.” Anticancer research vol. 40,10 (2020): 5687-5700. doi:10.21873/anticanres.14583
Article Snippet: “CD3-depleted PBMCs (CD3–PBMCs) were obtained using Dynabeads CD3 (Invitrogen Dynal AS, Oslo, Norway), and were suspended at 5×105 cells/ml in KBM-501 culture media (Kohjin Bio co. Ltd., Sakado, Japan)…”
Saito, Satoru et al. “Ex vivo generation of highly purified and activated natural killer cells from human peripheral blood.” Human gene therapy methods vol. 24,4 (2013): 241-52. doi:10.1089/hgtb.2012.183
Article Snippet: “Primary NK cells, PBMCs, CD3-PBMCs, CD14-PBMCs, and CD19-PBMCs were cultured in six-well plates (Nalge Nunc International K.K., Tokyo, Japan) at a concentration of 5×105cells/mL in KBM501…“
Comparison Data
Performance Evaluation of KBM 501:
Cell: Normal human peripheral blood monocytes
Vessel:12-well plate where anti-human-CD3 antibody is immobilized.
Cytokine: Recombinant human interleukin-2 (2814IU/mL)
Plasma: 1% inactivated autoplasma was added at the onset of culture.
Proliferative test: The culture was started at a seeding density of 27×10^4 cells/mL, under static conditions at 37℃, 5% CO2. The culture was diluted by adding each medium containing plasma upon cell proliferation. At day 10, cells unstained by trypan blue were counted.
Analysis: At day 10, the surface markers of activated lymphocytes were analyzed using flow cytometry, and cytotoxicity of K562 cells was measured with TeraScan which is a micro fluoro cytometer developed for measuring cytotoxicity.
Product Specifications
| Product No. | Product Name | Size | Shelf time | Storage |
|---|---|---|---|---|
| 16025015 | KBM 501 （IL-2：2810 IU/mL） | 500 mL | 8 months | 2-8℃ |
| 16025020 | KBM 502 （IL-2： 281 IU/mL） | 1000 mL | 8 months | 2-8℃ |
| 1602502B | KBM 502B（IL-2： 281 IU/mL） | 1000 mL | 8 months | 2-8℃ |
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