🔥 Don’t Miss Out on our Blind Box Giveaway with a minimum spend of $280! 🔥 Don’t Miss Out on our Blind Box Giveaway with a minimum spend of $280! 🔥 Don’t Miss Out on our Blind Box Giveaway with a minimum spend of $280!
🔥 Don’t Miss Out on our Blind Box Giveaway with a minimum spend of $280! 🔥 Don’t Miss Out on our Blind Box Giveaway with a minimum spend of $280! 🔥 Don’t Miss Out on our Blind Box Giveaway with a minimum spend of $280!
Add friend

In Vivo Ready™ Anti-Mouse CD4 (GK1.5)- 1 mg

In Vivo Ready™ Anti-Mouse CD4 (GK1.5)- 1 mg

Shopping cart

Subtotal: $2,050.00

View Cart Checkout

In Vivo Ready™ Anti-Mouse CD4 (GK1.5)- 1 mg
In Vivo Ready™ Anti-Mouse CD4 (GK1.5)- 1 mg - Image 2

In Vivo Ready™ Anti-Mouse CD4 (GK1.5)- 1 mg

In Vivo Ready™ Anti-Mouse CD4 (GK1.5)- 1 mg

Add to cart
SKU: 40-0041-M001 Categories: Tags: Brand:

The GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.

The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Anti-Mouse CD4 clone RM4-5, will “compete” for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4.

Product Details

Name In Vivo Ready™ Anti-Mouse CD4 (GK1.5)
Cat. No. 40-0041
Alternative Names L3T4, T4
Gene ID 12504
Clone GK1.5
Isotype Rat IgG2b, κ
Reactivity Mouse
Cross Reactivity Syrian Hamster
Format In Vivo Ready™
Application Flow Cytometry, Functional Assays, IHCF, IP
Citations* Taniguchi RT, DeVoss JJ, Moon JJ, Sidney J, Sette A, Jenkins MK, and Anderson MS. 2012. Proc. Natl. Acad. Sci. 109: 7847-7852. (in vivo depletion)

Wolkers MC, Gerlach, C, Arens R, Janssen EM, Fitzgerald P, Schumacher TN, Medema JP, Green DR, and Schoenberger SP. 2012. Blood. 119:798-804. (in vivo depletion)

Lee L-F, Logronio K, Tu GH, Zhai W, Ni I, Mei L, Dilley J, Yu J, et al. 2012. Proc. Natl. Acad. Sci. 10.1073. (Flow cytometry).

Thornton EE, Looney MR, Bose O, Sen D, Sheppard D, Locksley R, Huang X, and Krummel MF. 2012. J. Exp. Med. 10:1084. (Immunohistochemistry – OCT embedded frozen tissue)

Stephen TL, Wilson BS, and Laufer TM. 2012. Proc. Natl. Acad. Sci. 109: 7415-7420. (Immunoprecipitation)

Hammerbeck CD and Hooper JW. 2011. J. Virol. 85(19) 9929-9944. (Flow cytometry – Syrian hamster).

Diamond MS, Kinder M, Matsushita H, Mashayekhi M, Dunn GP, Archambault JM, Lee H, Arthur CD, White JM, Kalinke U, Murphy KM, and Schreiber RD. 2011. J. Exp. Med. 208: 1989-2003. (in vitro blocking)

Kao H, Lin J, Littman DR, Shaw AS, and Allen PM. 2008. J. Immunol. 181: 8248-8257. (Immunoprecipitation)

Felix NJ, Donermeyer DL, Horvath S, Walters JJ, Gross M, suri A, and Allen PM. 2007. Nat. Immunol. 8:388-397. (in vitro blocking)

Application Key:FC = Flow Cytometry; FA = Functional Assays; ELISA = Enzyme-Linked Immunosorbent Assay; ICC = Immunocytochemistry; IF = Immunofluorescence Microscopy; IHC = Immunohistochemistry; IHC-F = Immunohistochemistry, Frozen Tissue; IHC-P = Immunohistochemistry, Paraffin-Embedded Tissue; IP = Immunoprecipitation; WB = Western Blot; EM = Electron Microscopy

*Tonbo Biosciences tests all antibodies by flow cytometry. Citations are provided as a resource for additional applications that have not been validated by Tonbo Biosciences. Please choose the appropriate format for each application and consult the Materials and Methods section for additional details about the use of any product in these publications.

Technical Data Sheet       View All Formats                    Request Custom/Bulk

Other Related Solutions

HOW CAN WE HELP YOU? Our specialists are to help you find the best product for your application. We will be happy to help you find the right product for the job.

TALK TO A SPECIALIST

Contact our Customer Care, Sales & Scientific Assistance

EMAIL US

Consult and asked questions about our products & services

DOCUMENTATION

Documentation of Technical & Safety Data Sheet, Guides and more...

NEWSLETTER

We gladly support you by keeping you updated on our latest products and the developments around our services.

Our Featured Solutions

Animal Models (Preclinical)
Bioprocess
Cell Culture
Cell Imaging & Analysis
Cells & Tissue Repository
Exosome
Growth Factors, Recombinant Proteins
Laboratory Instruments & Equipements
Microfluidics
Western Blotting and Protein Analysis

SERVICES

Customised Soft Hydrogels
Data Analytics
DNA Synthesis Services
GMP Industrial/Clinical Lab Set Up
Innovation Enabler
NTA Particle Analysis
Preclinical and Translational Research
Skin Testing

RESEARCH FIELD

Food & Alternative Protein
Aging & Neuroscience
Cell & Gene Therapy
Drug Discovery & Development
Skin & Personal Care

LEARNING CENTRE

Blog
Technical Content
Product Information

ATLANTIS BIOSCIENCE PTE LTD

362 Upper Paya Lebar Rd, #07-15,
Singapore 534963

+65-8608 0974

[email protected]

LINE info

Linkedin Facebook Youtube

© 2015-2023 Atlantis Bioscience Pte Ltd. All rights reserved. Co Reg No: 201539516N