Hoechst 33258

Add to cart

Biotium products are distributed only in Singapore and Thailand.

A red fluorescent dye to covalently label dead cells, allowing cells with permeable plasma membranes to be detected in flow cytometry and microscopy.

SIZE	CATALOG #	PRICE
10 mL	#40044	$160
100 mL	#40045	$151

PRODUCT ATTRIBUTES

CAS number	23491-45-4, 23491-52-3
Cellular localization	Nucleus
For live or fixed cells	For fixed cells, For live/intact cells
Assay type/options	DNA content/cell cycle (flow cytometry), Long term staining (24-72h), No-wash staining, Real-time imaging
Cell permeability	Membrane permeant
Apoptosis/viability marker	All cell stain
Fixation options	Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)
Colors	Blue
Excitation/Emission	350/461 nm (with DNA), 352/461 nm (with DNA)

Hoechst Products

Product	Catalog Number	MW	Unit Size	λEx/λEm	Format
Hoechst 33258, 10 mg/mL in Water	40044	533.88	10 mL	352/461 nm	Yellow solution
Hoechst 33258, pentahydrate	40045	623.96	100 mg	352/461 nm	Yellow solid
Hoechst 33342, 10 mg/mL in Water	40046	561.93	10 mL	350/461 nm	Yellow solution
Hoechst 33342, trihydrochloride trihydrate	40047	615.98	100 mg	350/461 nm	Yellow solid

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains. Biotium offers Hoechst 33258 and Hoechst 33342 dyes in both solution or powder forms. Both dyes are spectrally similar but Hoechst 33528 is slightly more water soluble than Hoechst 33342. The dyes can be used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required.

Hoechst can also be used to stain live bacteria (gram-positive and gram-negative), but in live yeast the staining is weak and not nuclear. See our Cellular Stains Table for more information on how our dyes stain various organisms.