Product Description

HRP-Catalyzed Tyramide Kits for Multiplex IHC, CycIF & Spatial Biology

Boost immunofluorescence sensitivity by up to 100-fold using HRP-catalyzed Tyramide Signal Amplification (TSA), also known as Catalyzed Reporter Deposition (CARD).

Designed for multiplex IHC, ICC, FISH, CycIF and spatial biology workflows, these TSA kits enable high-density in situ labeling of low-abundance protein and nucleic acid targets — even in challenging samples such as human FFPE tissues.

An excellent alternative to Opal® and Aluora® tyramide systems.

Why Choose These TSA Kits?

Up to 100× Higher Sensitivity

• Detect low-expression biomarkers
• Improve signal in autofluorescent tissues
• Reduce primary antibody concentration

Flexible Tyramide Options

Choose from:
• 6 bright, photostable CF® Dye tyramides
• Biotin tyramide

CF® dyes deliver superior brightness and photostability compared to many conventional fluorophores.

Choose Your HRP Conjugate

• Goat anti-mouse HRP (highly cross-adsorbed)
• Goat anti-rabbit HRP
• Streptavidin-HRP

Compatible with multiplex and cyclic immunofluorescence (CycIF, t-CycIF).

Kit Components

Each kit includes:
• CF® Dye or Biotin-Tyramide stock solution
• HRP Goat anti-mouse IgG (highly cross-adsorbed*)
• BSA for blocking
• Tyramide Amplification Buffer Plus
• 30% Hydrogen Peroxide

Each kit stains approximately 50–150 slides, depending on protocol and volume used.

*Goat anti-mouse antibody is adsorbed against bovine, horse, human, rabbit, and swine serum to minimise cross-reactivity (not cross-adsorbed against rat).

Optimised for Multiplex & Cyclic Detection

TSA enables covalent dye deposition at the site of HRP activity, allowing:

• Multiple sequential rounds of amplification
• No host species limitation in multiplex panels
• Strong signal over background autofluorescence
• Integration with conventional immunostaining

Suitable for spatial biology platforms including Akoya PhenoImager systems.

Applications

✔ Multiplex IHC
✔ ICC
✔ FISH
✔ CycIF and t-CycIF
✔ Human FFPE tissue
✔ Low-abundance biomarker detection
✔ Spatial biology panel development

Also Available

• HRP Goat Anti-Rabbit TSA Kits
• HRP Streptavidin TSA Kits
• Ready-to-Use Amplification Buffer
• Tyramide Amplification Buffer Plus
• TyraMax™ Amplification Dyes for next-generation TSA performance

For a deeper understanding of panel design, workflow optimisation and multiplex strategy, read our practical guide on TSA-Based Multiplex Immunofluorescence in Spatial Biology here: https://www.atlantisbioscience.com/blog/tsa-multiplex-immunofluorescence-spatial-biology/

Also explore TyraMax™ Amplification Dyes, our next-generation tyramide reagents offering even brighter signal, improved photostability, and superior working solution stability compared to Opal® and Aluora®.

Note: Biotium products are distributed only in Singapore and Thailand.

Data

Specification