Biowest is the ideal partner for animal serum used for translational research in various clinical, academic and industrial accounts across the world.
All our serum are sourced from BSE , FMD and BVD regions. Our very own Singapore Veterinary Authority has inspected all documentation and permits before the final product reaches your lab.
The serum is tested for the presence of Chlortetracycline, Oxytetracycline and Tetracycline by a liquid chromatography electrospray ionisation tandem mass spectrometry method. The detection limit is <0.05mg/L. This test permits the full range of tetracycline regulated induction with well-characterized Tet cell lines.
Cell Culture Tests: Cell Growth, Plating Efficiency, Cloning Efficiency.
Cell Lines Tested
The following cell lines are tested with the serum: HELA -Cancer Cell/Human. L929 -Fibroblast-Mouse/ As Macrophage SP2/O-AG14 -Mouse/Lymphoma MRC- 5 -Human/Lung.
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- Our tet-free grade for inducible systems and gene expression studies, generally lower endotoxin than most brands(≤10 EU/mL endotoxin)
- Sterile filtered; mycoplasma and virus tested
- Proven success across wide spectrum of cell lines across several markets
- Widely accepted in key universities like NUS, CSI ( Singapore) and various RND labs across SEA.
- All products are produced in ISO 9001:2008 and ISO 13485:2012 facilities.
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- FBS that is functionally tested with our tetracycline expression systems (vectors) use for TET on and off system)
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[accordion title=”MSDS/Tech Sheet”] s181T_MSDS.pdf
s181T_techsheet.pdf [/accordion]
[accordion title=”Product Brochure”] serum brochure [/accordion]
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Reference Publications
Lu Wang, Tze King Tan, Hyoju Kim, Dennis Kappei, Shi Hao Tan, A. Thomas Look, Takaomi Sanda
ASCL1 characterizes adrenergic neuroblastoma via its pioneer function and cooperation with core regulatory circuit factors
Cell Reports, Volume 42, Issue 12, 2023, 113541, ISSN 2211-1247,
DOI: https://doi.org/10.1016/j.celrep.2023.113541
Article Snippet: “GI-MEN cell lines transduced with the DOX-inducible pCW57.1-ASCL1 vector were cultured in RPMI 1640 medium (Biowest, cat# L0500-500) supplemented with 10% tetracycline-free fetal bovine serum (Biowest, cat# S181T-500) and 1% penicillin/streptomycin (Biowest, cat# L0022-020).”
Radic Shechter, Ksenija et al.
Metabolic memory underlying minimal residual disease in breast cancer
Molecular systems biology vol. 17,10 (2021): e10141.
DOI: doi:10.15252/msb.202010141
Article Snippet: Highlight from the materials and methods table “Fetal Bovine Serum (FBS), Tetracycline Free Biowest S181T“
Marchena-Cruz, Esther et al.
DDX47, MeCP2, and other functionally heterogeneous factors protect cells from harmful R loops
Cell reports vol. 42,3 (2023): 112148.
DOI: doi:10.1016/j.celrep.2023.112148
Article Snippet: “U2OS-TR-AID stable cell line carrying a tetracycline-regulated AID gene cassette was cultured under the same conditions but supplemented with a special Fetal Bovine Serum (FBS) South America, Tetracycline Free (S181T, Biowest). Cells were grown at 37°C and 5% CO2.”
Turan, Gizem & Olgun, Cagla & Ayten, Hazal & Toker, et al.
Dynamic proximity interaction profiling suggests that YPEL2 is involved in cellular stress surveillance
bioRxiv 2023.07.31.551286.
DOI: 10.1101/2023.07.31.551286.
Article Snippet: “COS7 cells were maintained and transfected in the DMEM medium containing 10% tetracycline-free FBS (Tet-free FBS, Biowest, France, S181T) to minimize the effect of endogenous tetracycline on transgene expression.”