ExoBrite™ True EVMembrane Stains

Next-Generation Lipophilic Dyes for Reliable Pan-EV Staining

ExoBrite™ True EV Membrane Stains are advanced lipophilic dyes engineered specifically for extracellular vesicle (EV) research. Unlike conventional membrane dyes such as PKH, DiO, and DiI, ExoBrite™ stains deliver near-complete EV coverage with minimal aggregation, ensuring accurate EV detection across diverse experimental workflows.

These dyes are validated on leading EV analysis platforms, including Particle Metrix ZetaView® and Spectradyne ARC™ Particle Analyzer, making them the gold standard for fluorescence nanoparticle tracking analysis (fNTA) and flow cytometry-based EV detection.

Available in four distinct colours (Pacific Blue®, FITC, PE, and APC channels), ExoBrite™ dyes support pan-EV staining from multiple biological sources, while remaining fully compatible with antibody co-staining for multiplex applications.

Whether you’re tracking EV uptake, validating isolation workflows, or profiling EV subpopulations, ExoBrite™ empowers researchers to detect EVs—not dye aggregates.

Key Features

Lipophilic dyes optimised for pan-EV membrane labelling
Superior alternative to PKH, DiO, DiI, and DiD
Near-complete EV coverage with reduced aggregation
Broad compatibility with EVs from different biological sources
Supports antibody co-staining for multiplex analysis
Validated on ZetaView® and Spectradyne ARC™ instruments
Available in four colours matching major flow cytometry channels

Proven Results: Significantly Greater Coverage of EVs Over Other Membrane Dyes Such as PKH, DiO, and DiI

Bar chart showing Significantly Greater Coverage of EVs Over Other Membrane Dyes Such as PKH, DiO, and DiI — SEC purified MCF-7-derived EVs were stained with three green membrane dyes: ExoBrite™ 515/540 True EV Membrane Stain, PKH67, and Neuro-DiO. The stained EVs were diluted and run on a ZetaView® particle analyzer. Total EV number was measured in scatter mode, and fluorescently-stained EV number was measured with 488 nm excitation and 500 nm filter. The percentage of particles stained with the dye is shown in the plot. ExoBrite™ 515/540 True EV Membrane Stain showed nearly complete coverage of EVs, much higher than PKH67 or Neuro-DiO.

Bar chart showing comparison of % of EVs stained with dye between ExoBrite™ 555/575 True EV Membrane Stain and DiI. — SEC purified MCF-7-derived EVs were stained with two orange membrane dyes: ExoBrite™ 555/575 True EV Membrane Stain, and DiI. The stained EVs were diluted and run on a ZetaView® particle analyzer. Total EV number was measured in scatter mode, and fluorescently-stained EV number was measured with 520 nm excitation and 550 nm filter. The percentage of particles stained with the dye is shown in the plot. ExoBrite™ 555/575 True EV Membrane Stain showed nearly complete coverage of EVs, much higher than DiI.

Proven Results: ExoBrite™ True EV Membrane Stain Outperforms PKH67 for EV Detection on ARC™ Particle Analyzer

ExoBrite™ 515/540 True EV Membrane Stain is more effective at staining EVs and is less susceptible to dye aggregation than PKH67 membrane dye. — Fluorescence analysis of HEK-293 cell-derived EVs stained with ExoBrite™ 515/540 True EV Membrane Stain and PKH67. The results indicate ExoBrite™ 515/540 True EV Membrane Stain is more effective at staining EVs than PKH67 membrane dye (62% vs. 49%, respectfully). Particle analysis was performed using Spectradyne’s ARC™ Particle Analyzer. Figure courtesy of Spectradyne LLC.

???? Explore the True EV Membrane Stain today – four colours are awaiting you!

Note: Biotium products are available only in Singapore and Thailand.

*Please leave us a message during checkout to indicate which stain you need, and our team will process your order accordingly.

Variations

Product	Ex/Em (nm)	Detection Channels	Size Options	Catalog Number
ExoBrite™ 400/460 True EV Membrane Stain	402/460 nm	Pacific Blue®	100 Labelings / 500 Labelings	30136-T / 30136
ExoBrite™ 515/540 True EV Membrane Stain	515/542 nm	FITC	100 Labelings / 500 Labelings	30129-T / 30129
ExoBrite™ 555/575 True EV Membrane Stain	556/576 nm	PE	100 Labelings / 500 Labelings	30130-T / 30130
ExoBrite™ 645/675 True EV Membrane Stain	644/671 nm	APC	100 Labelings / 500 Labelings	30137-T / 30137