Optimised Kit for Total RNA (mRNA + miRNA) Extraction from Purified EVs

The ExoBrite™ EV Total RNA Isolation Kit is designed for efficient extraction of total RNA, including mRNA and miRNA, from purified extracellular vesicles (EVs).

EV samples often yield low RNA quantities due to their dilute nature and small RNA cargo. This kit overcomes those challenges with an easy-to-use, column-based method that avoids phenol/chloroform and ethanol precipitation steps. The result is high-quality RNA, suitable for downstream applications such as qPCR or RNAseq.

Validated to recover ~10–20 ng of RNA from 1×10¹⁰ SEC-enriched EVs, this kit ensures reliable preparation of both long and short RNA species, including non-coding RNAs like miRNAs, which are critical for biomarker discovery and EV research.

The protocol is simple, taking as little as 20 minutes, with an optional DNase treatment step to remove contaminating DNA for cleaner results.

Key Features

• Extract total RNA (mRNA + miRNA) from purified EVs
• Recovers ~10–20 ng RNA from 1×10¹⁰ SEC-enriched EVs
• Compatible with qPCR and RNAseq workflows
• Simple column-based method — 20-minute prep time
• No phenol/chloroform or ethanol precipitation
• Includes optional DNase treatment for DNA removal

Kit Components

• EV Lysis Buffer
• Column Binding Buffer
• DNase Buffer
• DNase I (lyophilised)
• Column Wash Buffer
• Spin Columns

Notes: Biotium products are available only in Singapore and Thailand.

Streamline your EV RNA workflows with ExoBrite™ EV Total RNA Isolation Kit – high-quality RNA ready for qPCR or RNAseq.

*Please leave us a message during checkout to indicate which kit you need, and our team will process your order accordingly.