Bright EV Stains with Excellent Signal-to-Noise

ExoBrite™ CTB EV Staining Kits are fluorescent conjugates of cholera toxin subunit B (CTB), optimised to provide bright, specific staining of extracellular vesicles (EVs) while overcoming common challenges in EV detection.

Unlike many lipophilic dyes that form aggregates or bind non-specifically to polystyrene beads, ExoBrite™ CTB stains are formulated to deliver high fluorescence intensity with little to no background aggregation. This makes them highly reliable for flow cytometry detection of both purified EVs and bead-bound EVs.

By binding to GM1 gangliosides commonly found on EV membranes, ExoBrite™ CTB stains ensure superior signal-to-noise ratio and more complete coverage compared to traditional stains such as DiO, CellMask™, ExoFlow-ONE™, and ExoGlow™. Fully compatible with antibody co-staining (e.g. CD9, CD63, CD81), these stains enable multi-parameter EV analysis with confidence.

Each kit contains ExoBrite™ CTB EV Stain and Reconstitution Solution, yielding 100 µL of 500X staining solution per vial.

Key Features

• Optimally formulated CTB conjugates for EV staining
• Bright fluorescence and low background for excellent signal-to-noise
• Works with both purified and bead-bound EVs
• Compatible with flow cytometry and antibody co-staining
• Available in four detection channel options (Pacific Blue™, FITC, PE/Cy®3, APC)

Less Background, Better Coverage

ExoBrite™ CTB EV Stains deliver exceptional signal-to-noise and more complete coverage of purified and bead-bound EVs compared to other stains. In contrast, lipophilic dyes like DiO and CellMask™ show significant aggregation, while stains such as ExoFlow-ONE™ and ExoGlow™ provide less EV coverage.

Kit Components

• ExoBrite™ CTB EV Stain
• ExoBrite™ Reconstitution Solution
• Each vial makes 100 µL of 500X staining solution

Notes: Biotium products are available only in Singapore and Thailand.

Enhance your EV research with ExoBrite™ CTB EV Staining Kits – designed for unmatched signal clarity and versatility.

*Please leave us a message during checkout to indicate which kit you need, and our team will process your order accordingly.