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ExoBrite™ CD9 Western Antibody

ExoBrite™ CD9 Western Antibody

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ExoBrite™ CD9 Western Antibody cover image
Chemiluminescent western detection of CD9 in 10 ug of MCF7 whole cell lysate (non-reduced) using 25 ng/mL ExoBrite™ CD9-HRP conjugate, detected with SuperSignal™ West Femto Substrate (Thermo Fisher) and imaged on a LICORbio™ Odyssey® M imaging system.
Western detection of human CD9 in cell and exosome lysate using ExoBrite™ 680/700 CD9 Western Antibody, showing enrichment of CD9 in the exosome prep. Exosomes were isolated from MCF-7 cell conditioned medium by size exclusion column. The indicated amounts of lysates from MCF-7 cells and MCF-7-derived exosomes were run on an acrylamide gel and transferred to PVDF. The membrane was blocked with TrueBlack® Western Blocking Buffer, stained with 1X ExoBrite™ 680/700 CD9 Western Antibody, and imaged on a LI-COR Odyssey® infrared imaging system in the 700 channel. The protein ladder is Peacock™ Prestained Protein Marker, 1.5 uL per lane.
Western blot showing CD9 and CD63 enrichment in exosomes using ExoBrite™ Western Antibodies in MCF-7 cell and exosome lysates. Exosomes were isolated from MCF-7 cell conditioned medium by size exclusion column. The indicated amounts of lysates from MCF-7 cells and MCF-7-derived exosomes were run on an acrylamide gel and transferred to PVDF. The membrane was blocked with TrueBlack® Western Blocking Buffer, stained with 1X ExoBrite™ 680/700 CD63 Western Antibody and 1X ExoBrite™ 770/800 CD9 Western Antibody, and imaged on a LI-COR Odyssey® infrared imaging system in the 700 and 800 channels. The protein ladder is Peacock™ Plus Prestained Protein Marker, 3 uL loaded.
Western blot showing CD9 enrichment in exosomes using ExoBrite™ Western Antibodies in MCF-7 cell and exosome lysates. Exosomes were isolated from MCF-7 cell conditioned medium by size exclusion column. The indicated amounts of lysates from MCF-7 cells and MCF-7-derived exosomes were run on an acrylamide gel and transferred to PVDF. The membrane was blocked with TrueBlack® Western Blocking Buffer, stained with 1X ExoBrite™ 680/700 CD9 Western Antibody and 1X ExoBrite™ 770/800 Calnexin Western Antibody, and imaged on a LI-COR Odyssey® infrared imaging system in the 700 and 800 channels. The protein ladder is Peacock™ Prestained Protein Marker, 3 uL loaded.

ExoBrite™ CD9 Western Antibody

ExoBrite™ CD9 Western Antibody delivers sensitive, low-background detection of EV marker CD9 in western blots, available in fluorescent and HRP formats.

ExoBrite™ CD9 Western Antibody cover image

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SKU: P003-680, P003-770 Categories: Tags: Brand:

Validated Antibody for EV Marker CD9 Detection by Fluorescent or Chemiluminescent Western Blot

ExoBrite™ CD9 Western Antibody is a mouse monoclonal IgG1, kappa antibody validated for the detection of CD9 in extracellular vesicle (EV) extracts by western blot. Available in near-infrared fluorescent formats (680/700 and 770/800) and an HRP-conjugated chemiluminescent format, it enables sensitive, low-background detection of EV-associated CD9.

The near-infrared fluorescent conjugates provide greater signal-to-noise than visible light fluorophores, making them ideal for multiplex western blotting. The HRP-conjugated format supports chemiluminescent detection for standard EV protein analysis workflows.

By targeting CD9, a widely used EV tetraspanin marker, this antibody supports robust characterisation of exosome and EV preparations, complementing ExoBrite™ antibodies for CD63 and CD81.

Key Features

  • Validated for EV marker CD9 detection in western blots
  • Formats available: near-infrared fluorescent (680/700, 770/800) and HRP
  • Low background and high signal-to-noise performance
  • Multiplex capability with near-infrared detection
  • Complements ExoBrite™ CD63 and CD81 antibodies for EV profiling

 

Notes: Biotium products are available only in Singapore and Thailand.

 

Detect CD9 with confidence in EV extracts using ExoBrite™ CD9 Western Antibody – available in fluorescent and HRP formats.

*Please leave us a message during checkout to indicate which kit you need, and our team will process your order accordingly.

Product Attributes

Attribute Details
Antibody number P003
SwissProt P21926
Antibody type Primary
Clonality Monoclonal
Host species Mouse
Isotype IgG1, kappa
Antibody reactivity (target) CD9
Synonyms Tetraspanin-29 (TSPAN29), BA-2/p24 antigen, MRP1, MIC3, DRAP27, GIG2
Species reactivity Human, Baboon, Bovine, Dog, Horse, Rabbit, Sheep, Cynomolgus monkey
Human gene symbol CD9
Entrez gene ID 928
Unigene 114286
Molecular weight 24 kDa
Target cellular localisation Exosomes/EVs, Plasma membrane
Cell/tissue expression Exosomes, Platelets, Basophils, Eosinophils, Epithelial cells, Lymphocytes
Verified applications Western blot (fluorescent or chemiluminescent, verified)
Positive control MCF-7 cells, MCF-7 derived exosomes
Recommended use ~100 ng/mL (1:1000 dilution for WB, adjust as required by end-user)
Conjugate formulation Fluorescent conjugates: PBS, 0.1% BSA, 0.05% azide; HRP conjugates: PBS, 50% glycerol, 2 mg/mL rBSA
Volume per assay 10 µL/test
Shelf life ≥ 24 months from receipt (if stored as recommended)
Storage conditions Store at 2–8 °C, protect fluorescent conjugates from light
Shipping condition Room temperature
Regulatory status Research Use Only (RUO)
Product origin May contain BSA (bovine serum) or recombinant BSA (CHO cells). Inquire for lot-specific details.

Variations

Antibody Ex/Em (nm) Concentration Size Options Detection Channel Catalog No.
ExoBrite™ 680/700 CD9 Western Antibody 681/698 100 µg/mL 25 / 100 tests NIR (700 channel) P003-680-250 / P003-680-1000
ExoBrite™ 770/800 CD9 Western Antibody 770/797 100 µg/mL 25 / 100 tests NIR (800 channel) P003-770-250 / P003-770-1000

Documentation

Proven Performance

Western blot showing CD9 enrichment in exosomes using ExoBrite™ Western Antibodies in MCF-7 cell and exosome lysates. Exosomes were isolated from MCF-7 cell conditioned medium by size exclusion column. The indicated amounts of lysates from MCF-7 cells and MCF-7-derived exosomes were run on an acrylamide gel and transferred to PVDF. The membrane was blocked with TrueBlack® Western Blocking Buffer, stained with 1X ExoBrite™ 680/700 CD9 Western Antibody and 1X ExoBrite™ 770/800 Calnexin Western Antibody, and imaged on a LI-COR Odyssey® infrared imaging system in the 700 and 800 channels. The protein ladder is Peacock™ Prestained Protein Marker, 3 uL loaded.
Western blot showing CD9 enrichment in exosomes using ExoBrite™ Western Antibodies in MCF-7 cell and exosome lysates. Exosomes were isolated from MCF-7 cell conditioned medium by size exclusion column. The indicated amounts of lysates from MCF-7 cells and MCF-7-derived exosomes were run on an acrylamide gel and transferred to PVDF. The membrane was blocked with TrueBlack® Western Blocking Buffer, stained with 1X ExoBrite™ 680/700 CD9 Western Antibody and 1X ExoBrite™ 770/800 Calnexin Western Antibody, and imaged on a LI-COR Odyssey® infrared imaging system in the 700 and 800 channels. The protein ladder is Peacock™ Prestained Protein Marker, 3 uL loaded.
Chemiluminescent western detection of CD9 in 10 ug of MCF7 whole cell lysate (non-reduced) using 25 ng/mL ExoBrite™ CD9-HRP conjugate, detected with SuperSignal™ West Femto Substrate (Thermo Fisher) and imaged on a LICORbio™ Odyssey® M imaging system.
Chemiluminescent western detection of CD9 in 10 ug of MCF7 whole cell lysate (non-reduced) using 25 ng/mL ExoBrite™ CD9-HRP conjugate, detected with SuperSignal™ West Femto Substrate (Thermo Fisher) and imaged on a LICORbio™ Odyssey® M imaging system.
Western detection of human CD9 in cell and exosome lysate using ExoBrite™ 680/700 CD9 Western Antibody, showing enrichment of CD9 in the exosome prep. Exosomes were isolated from MCF-7 cell conditioned medium by size exclusion column. The indicated amounts of lysates from MCF-7 cells and MCF-7-derived exosomes were run on an acrylamide gel and transferred to PVDF. The membrane was blocked with TrueBlack® Western Blocking Buffer, stained with 1X ExoBrite™ 680/700 CD9 Western Antibody, and imaged on a LI-COR Odyssey® infrared imaging system in the 700 channel. The protein ladder is Peacock™ Prestained Protein Marker, 1.5 uL per lane.
Western detection of human CD9 in cell and exosome lysate using ExoBrite™ 680/700 CD9 Western Antibody, showing enrichment of CD9 in the exosome prep. Exosomes were isolated from MCF-7 cell conditioned medium by size exclusion column. The indicated amounts of lysates from MCF-7 cells and MCF-7-derived exosomes were run on an acrylamide gel and transferred to PVDF. The membrane was blocked with TrueBlack® Western Blocking Buffer, stained with 1X ExoBrite™ 680/700 CD9 Western Antibody, and imaged on a LI-COR Odyssey® infrared imaging system in the 700 channel. The protein ladder is Peacock™ Prestained Protein Marker, 1.5 uL per lane.
Western blot showing CD9 and CD63 enrichment in exosomes using ExoBrite™ Western Antibodies in MCF-7 cell and exosome lysates. Exosomes were isolated from MCF-7 cell conditioned medium by size exclusion column. The indicated amounts of lysates from MCF-7 cells and MCF-7-derived exosomes were run on an acrylamide gel and transferred to PVDF. The membrane was blocked with TrueBlack® Western Blocking Buffer, stained with 1X ExoBrite™ 680/700 CD63 Western Antibody and 1X ExoBrite™ 770/800 CD9 Western Antibody, and imaged on a LI-COR Odyssey® infrared imaging system in the 700 and 800 channels. The protein ladder is Peacock™ Plus Prestained Protein Marker, 3 uL loaded.
Western blot showing CD9 and CD63 enrichment in exosomes using ExoBrite™ Western Antibodies in MCF-7 cell and exosome lysates. Exosomes were isolated from MCF-7 cell conditioned medium by size exclusion column. The indicated amounts of lysates from MCF-7 cells and MCF-7-derived exosomes were run on an acrylamide gel and transferred to PVDF. The membrane was blocked with TrueBlack® Western Blocking Buffer, stained with 1X ExoBrite™ 680/700 CD63 Western Antibody and 1X ExoBrite™ 770/800 CD9 Western Antibody, and imaged on a LI-COR Odyssey® infrared imaging system in the 700 and 800 channels. The protein ladder is Peacock™ Plus Prestained Protein Marker, 3 uL loaded.

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