Optimised 3-Color Antibody Mixtures for EV Phenotyping by Flow Cytometry
ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktails are pre-optimised antibody mixtures designed for fast and reliable phenotyping of extracellular vesicles (EVs) by flow cytometry.
Each cocktail combines the three most widely used EV tetraspanin markers—CD9, CD63, and CD81—into a cost-effective mixture formulated for optimal brightness, low aggregation, and high signal-to-noise. By eliminating the need for antibody optimisation, these cocktails enable researchers to obtain reproducible EV profiling results more quickly.
Two formulations are available: Blue/Green/Far-Red and Green/Orange/Far-Red, supporting flexible panel design while preserving additional flow cytometry channels for multi-target analysis.
Key Features
- Designed for phenotyping EVs by flow cytometry
- Includes CD9, CD63, and CD81 for broad EV coverage
- Each antibody optimised for bright signal and low aggregation
- Cost-efficient mixture vs purchasing individual conjugates
- Two cocktail options: Blue/Green/Far-Red and Green/Orange/Far-Red
- Compatible with other antibody panels for multi-parameter EV analysis
Notes: Biotium products are available only in Singapore and Thailand.
Profile EVs quickly and confidently with ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktails – optimised for brightness and reproducibility.
*Please leave us a message during checkout to indicate which kit you need, and our team will process your order accordingly.
Product Attributes
Attribute | Details |
---|---|
Antibody numbers | P028, P029 |
SwissProt | P21926 (CD9), P08962 (CD63), P60033 (CD81) |
Antibody type | Primary |
Clonality | Monoclonal |
Host species | Mouse |
Isotype | IgG1, kappa |
Antibody reactivity (targets) | CD9, CD63, CD81 |
Species reactivity | Human |
Human gene symbols | CD9, CD63, CD81 |
Entrez gene IDs | 928, 967, 975 |
Unigene | 114286, 445570, 54457 |
Target localisation | Exosomes/EVs, Plasma membrane |
Verified applications | Flow cytometry (surface staining, verified) |
Positive control | MCF-7 cells, MCF-7 derived EVs |
Recommended concentration | 5 µL per 0.1 mL exosomes |
Conjugate formulation | Proprietary buffer containing 0.05% sodium azide |
Shelf life | ≥ 24 months from receipt (if stored as recommended) |
Storage conditions | Store at 2–8 °C, protect fluorescent conjugates from light |
Shipping condition | Room temperature |
Regulatory status | Research Use Only (RUO) |
Variations
Cocktail | Ex/Em (nm) | Detection Channel(s) | Size Options | Catalog No. |
---|---|---|---|---|
ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktail (Blue/Green/Far-Red) | 490/516, 562/584, 642/663 | FITC, PE, APC | 25 tests / 100 tests | P028-125 / P028-500 |
ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktail (Green/Orange/Far-Red) | 490/516, 562/584, 642/663 | FITC, PE, APC | 25 tests / 100 tests | P029-125 / P029-500 |
Proven Performance

