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ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktails

ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktails

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ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktails cover image
SEC-purified HEK 293-derived EVs were stained with ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktail (Blue/Green/Far-Red) and clear staining was seen in all 3 channels (top row). The same antibody cocktail was run in PBS, and very few aggregates are seen (bottom row). Staining was detected in the V450 (Pacific Blue), B525 (FITC), and R660 (APC) channels.
SEC-purified HEK 293-derived EVs were stained with ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktail (Green/Orange/Far-Red) and clear staining was seen in all 3 channels (top row). The same antibody cocktail was run in PBS, and very few aggregates are seen (bottom row). Staining was detected in the B525 (FITC), Y585 (PE), and R660 (APC) channels.

ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktails

ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktails deliver fast, reproducible EV phenotyping by flow cytometry with optimised antibody mixtures.

ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktails cover image

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SKU: P028, P029 Categories: Tags: Brand:

Optimised 3-Color Antibody Mixtures for EV Phenotyping by Flow Cytometry

ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktails are pre-optimised antibody mixtures designed for fast and reliable phenotyping of extracellular vesicles (EVs) by flow cytometry.

Each cocktail combines the three most widely used EV tetraspanin markers—CD9, CD63, and CD81—into a cost-effective mixture formulated for optimal brightness, low aggregation, and high signal-to-noise. By eliminating the need for antibody optimisation, these cocktails enable researchers to obtain reproducible EV profiling results more quickly.

Two formulations are available: Blue/Green/Far-Red and Green/Orange/Far-Red, supporting flexible panel design while preserving additional flow cytometry channels for multi-target analysis.

Key Features

  • Designed for phenotyping EVs by flow cytometry
  • Includes CD9, CD63, and CD81 for broad EV coverage
  • Each antibody optimised for bright signal and low aggregation
  • Cost-efficient mixture vs purchasing individual conjugates
  • Two cocktail options: Blue/Green/Far-Red and Green/Orange/Far-Red
  • Compatible with other antibody panels for multi-parameter EV analysis

 

Notes: Biotium products are available only in Singapore and Thailand.

 

Profile EVs quickly and confidently with ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktails – optimised for brightness and reproducibility.

*Please leave us a message during checkout to indicate which kit you need, and our team will process your order accordingly.

Product Attributes

Attribute Details
Antibody numbers P028, P029
SwissProt P21926 (CD9), P08962 (CD63), P60033 (CD81)
Antibody type Primary
Clonality Monoclonal
Host species Mouse
Isotype IgG1, kappa
Antibody reactivity (targets) CD9, CD63, CD81
Species reactivity Human
Human gene symbols CD9, CD63, CD81
Entrez gene IDs 928, 967, 975
Unigene 114286, 445570, 54457
Target localisation Exosomes/EVs, Plasma membrane
Verified applications Flow cytometry (surface staining, verified)
Positive control MCF-7 cells, MCF-7 derived EVs
Recommended concentration 5 µL per 0.1 mL exosomes
Conjugate formulation Proprietary buffer containing 0.05% sodium azide
Shelf life ≥ 24 months from receipt (if stored as recommended)
Storage conditions Store at 2–8 °C, protect fluorescent conjugates from light
Shipping condition Room temperature
Regulatory status Research Use Only (RUO)

Variations

Cocktail Ex/Em (nm) Detection Channel(s) Size Options Catalog No.
ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktail (Blue/Green/Far-Red) 490/516, 562/584, 642/663 FITC, PE, APC 25 tests / 100 tests P028-125 / P028-500
ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktail (Green/Orange/Far-Red) 490/516, 562/584, 642/663 FITC, PE, APC 25 tests / 100 tests P029-125 / P029-500

Documentation

Proven Performance

SEC-purified HEK 293-derived EVs were stained with ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktail (Blue/Green/Far-Red) and clear staining was seen in all 3 channels (top row). The same antibody cocktail was run in PBS, and very few aggregates are seen (bottom row). Staining was detected in the V450 (Pacific Blue), B525 (FITC), and R660 (APC) channels.
SEC-purified HEK 293-derived EVs were stained with ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktail (Blue/Green/Far-Red) and clear staining was seen in all 3 channels (top row). The same antibody cocktail was run in PBS, and very few aggregates are seen (bottom row). Staining was detected in the V450 (Pacific Blue), B525 (FITC), and R660 (APC) channels.
SEC-purified HEK 293-derived EVs were stained with ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktail (Green/Orange/Far-Red) and clear staining was seen in all 3 channels (top row). The same antibody cocktail was run in PBS, and very few aggregates are seen (bottom row). Staining was detected in the B525 (FITC), Y585 (PE), and R660 (APC) channels.
SEC-purified HEK 293-derived EVs were stained with ExoBrite™ CD9/CD63/CD81 3-Color Antibody Cocktail (Green/Orange/Far-Red) and clear staining was seen in all 3 channels (top row). The same antibody cocktail was run in PBS, and very few aggregates are seen (bottom row). Staining was detected in the B525 (FITC), Y585 (PE), and R660 (APC) channels.

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