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ExoBrite™ CD81 (Mouse/Rat) Flow Antibody

ExoBrite™ CD81 (Mouse/Rat) Flow Antibody

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ExoBrite™ CD81 (Mouse/Rat) Flow Antibody cover image
SEC-purified mouse NIH 3T3-derived EVs were stained with ExoBrite™ 410/450 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.
SEC-purified NIH 3T3-derived EVs were stained with ExoBrite™ APC CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified NIH 3T3-derived EVs were stained with ExoBrite™ 650/665 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified NIH 3T3-derived EVs were stained with ExoBrite™ 640/660 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified mouse NIH 3T3-derived EVs were stained with ExoBrite™ 560/585 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 490/515 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.

ExoBrite™ CD81 (Mouse/Rat) Flow Antibody

ExoBrite™ CD81 (Mouse/Rat) Flow Antibody provides bright, low-background detection of CD81 in purified or bead-bound EVs by flow cytometry.

ExoBrite™ CD81 (Mouse/Rat) Flow Antibody cover image

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SKU: P019 Categories: Tags: Brand:

Validated Antibody for Detection of Mouse and Rat EV Marker CD81

ExoBrite™ CD81 (Mouse/Rat) Flow Antibody is a hamster monoclonal IgG1, kappa antibody validated by Biotium for optimal detection of CD81, a widely used extracellular vesicle (EV) marker, in mouse and rat EVs by flow cytometry.

Designed for use with purified and bead-bound EVs, this antibody provides bright signal, low background, and excellent signal-to-noise. With an updated buffer formulation, ExoBrite™ CD81 (Mouse/Rat) Flow Antibody delivers reliable and reproducible results for EV analysis.

Available in five colour options (Pacific Blue™, FITC, PE, APC, and APC-R), this antibody offers flexible multi-parameter analysis and pairs well with other EV tetraspanins (CD9 and CD63).

Key Features

  • Specific detection of mouse and rat CD81 EV marker by flow cytometry
  • Bright fluorescence, low background
  • Validated for purified and bead-bound EVs
  • Five colour options for panel flexibility
  • Updated buffer formulation for enhanced signal-to-noise

Notes: Biotium products are available only in Singapore and Thailand.

 

Detect mouse and rat EVs with confidence using ExoBrite™ CD81 (Mouse/Rat) Flow Antibody – validated for purified and bead-bound vesicles.

*Please leave us a message during checkout to indicate which kit you need, and our team will process your order accordingly.

Product Attributes

Attribute Details
Antibody number P019
SwissProt P35762 (mouse), Q62745 (rat)
Antibody type Primary
Clonality Monoclonal
Host species Hamster (Cricetinae)
Isotype IgG1, kappa
Antibody reactivity (target) CD81
Synonyms Target of the antiproliferative antibody 1 (TAPA-1)
Species reactivity Mouse, Rat
Human gene symbol CD81
Entrez gene ID 12520 (mouse), 25621 (rat)
Molecular weight 26 kDa
Target cellular localisation Exosomes/EVs, Plasma membrane
Cell/tissue expression Exosomes, Hepatocytes, Lymphocytes, Monocytes/Macrophages
Verified applications Exosome staining (verified)
Positive control NIH 3T3 cells
Recommended concentration 5 µL per 0.1 mL exosomes (flow cytometry)
Research areas Exosomes/EVs
Conjugate formulation Proprietary buffer containing 0.05% sodium azide
Shelf life ≥ 24 months from receipt (if stored as recommended)
Storage conditions Store at 2–8 °C, protect fluorescent conjugates from light
Shipping condition Room temperature
Regulatory status Research Use Only (RUO)
Product origin May contain BSA (from bovine serum) or recombinant BSA (CHO cells). Inquire for lot-specific details.

 

Variations

Antibody Ex/Em (nm) Target Species Reactivity Detection Channel Catalog No.
ExoBrite™ 410/450 CD81 (Mouse/Rat) Flow Antibody 416/452 CD81 Mouse, Rat Pacific Blue™ P019-410
ExoBrite™ 490/515 CD81 (Mouse/Rat) Flow Antibody 490/516 CD81 Mouse, Rat FITC P019-490
ExoBrite™ 560/585 CD81 (Mouse/Rat) Flow Antibody 562/584 CD81 Mouse, Rat PE P019-560
ExoBrite™ 640/660 CD81 (Mouse/Rat) Flow Antibody 642/663 CD81 Mouse, Rat APC P019-640
ExoBrite™ APC CD81 (Mouse/Rat) Flow Antibody 651/660 CD81 Mouse, Rat APC P019-APC

Documentation

Proven Performance

SEC-purified mouse NIH 3T3-derived EVs were stained with ExoBrite™ 410/450 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.
SEC-purified mouse NIH 3T3-derived EVs were stained with ExoBrite™ 410/450 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.
SEC-purified NIH 3T3-derived EVs were stained with ExoBrite™ APC CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified NIH 3T3-derived EVs were stained with ExoBrite™ APC CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified NIH 3T3-derived EVs were stained with ExoBrite™ 650/665 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified NIH 3T3-derived EVs were stained with ExoBrite™ 650/665 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified NIH 3T3-derived EVs were stained with ExoBrite™ 640/660 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified NIH 3T3-derived EVs were stained with ExoBrite™ 640/660 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified mouse NIH 3T3-derived EVs were stained with ExoBrite™ 560/585 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
SEC-purified mouse NIH 3T3-derived EVs were stained with ExoBrite™ 560/585 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 490/515 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 490/515 CD81 (mouse/rat) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.

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