ExoBrite™ CD63 (Mouse) Flow Antibody

ExoBrite™ CD63 (Mouse) Flow Antibody

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ExoBrite™ CD9 (Mouse) Flow Antibody cover image
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 410/450 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.
SEC-purified mouse NIH 3T3-derived EVs were stained with ExoBrite™ 490/515 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 560/585 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 640/660 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 650/665 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified NIH 3T3-derived EVs were stained with ExoBrite™ APC CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.

ExoBrite™ CD63 (Mouse) Flow Antibody

ExoBrite™ CD63 (Mouse) Flow Antibody delivers bright, low-background detection of mouse CD63 in purified or bead-bound EVs by flow cytometry.

ExoBrite™ CD9 (Mouse) Flow Antibody cover image

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SKU: P022 Categories: Tags: Brand:

Validated Antibody for Reliable Detection of Mouse EV Marker CD63

ExoBrite™ CD63 (Mouse) Flow Antibody is a rat monoclonal IgG2a, kappa antibody validated by Biotium for detection of mouse CD63, a key extracellular vesicle (EV) marker, by flow cytometry.

Optimised for use with purified and bead-bound EVs, this antibody ensures bright signal, low background, and high signal-to-noise performance. With an updated buffer formulation, ExoBrite™ CD63 (Mouse) Flow Antibody delivers improved staining specificity and reproducibility.

Available in five colour options (Pacific Blue™, FITC, PE, APC, and APC-R), this antibody supports flexible panel design and can be combined with other EV tetraspanins (CD9 and CD81) for multi-parameter EV analysis.

Key Features

  • Specific detection of mouse CD63 EV marker by flow cytometry
  • Bright fluorescence, low background
  • Validated for purified and bead-bound EVs
  • Five colour options for panel flexibility
  • Updated buffer formulation for enhanced signal-to-noise

Notes: Biotium products are available only in Singapore and Thailand.

 

Detect mouse EVs with confidence using ExoBrite™ CD63 (Mouse) Flow Antibody – validated for purified and bead-bound vesicles.

*Please leave us a message during checkout to indicate which kit you need, and our team will process your order accordingly.

Product Attributes

Attribute Details
Antibody number P022
SwissProt P41731
Antibody type Primary
Clonality Monoclonal
Host species Rat (Rattus norvegicus)
Isotype IgG2a, kappa
Antibody reactivity (target) CD63
Synonyms LIMP, LAMP-3, gp55, ME491, Melanoma-associated antigen
Species reactivity Mouse
Human gene symbol CD63
Entrez gene ID 12512
Molecular weight 53 kDa
Target cellular localisation Exosomes/EVs, Lysosomes, Plasma membrane, Multivesicular bodies
Cell/tissue expression Exosomes, Platelets, Granulocytes, Lymphocytes, Monocytes/Macrophages
Verified applications Exosome staining (verified)
Positive control NIH 3T3 cells
Recommended concentration 5 µL per 0.1 mL exosomes (flow cytometry)
Research areas Exosomes/EVs
Conjugate formulation Proprietary buffer containing 0.05% sodium azide
Shelf life ≥ 24 months from receipt (if stored as recommended)
Storage conditions Store at 2–8 °C, protect fluorescent conjugates from light
Regulatory status Research Use Only (RUO)
Product origin May contain BSA (from bovine serum) or recombinant BSA (CHO cells). Inquire for lot-specific details.

Variations

Antibody Ex/Em (nm) Target Species Reactivity Detection Channel Catalog No.
ExoBrite™ 410/450 CD63 (Mouse) Flow Antibody 416/452 CD63 Mouse Pacific Blue™ P022-410
ExoBrite™ 490/515 CD63 (Mouse) Flow Antibody 490/516 CD63 Mouse FITC P022-490
ExoBrite™ 560/585 CD63 (Mouse) Flow Antibody 562/584 CD63 Mouse PE P022-560
ExoBrite™ 640/660 CD63 (Mouse) Flow Antibody 642/663 CD63 Mouse APC P022-640
ExoBrite™ APC CD63 (Mouse) Flow Antibody 651/660 CD63 Mouse APC P022-APC

Documentation

Proven Performance

SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 410/450 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 410/450 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.
SEC-purified mouse NIH 3T3-derived EVs were stained with ExoBrite™ 490/515 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.
SEC-purified mouse NIH 3T3-derived EVs were stained with ExoBrite™ 490/515 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 560/585 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 560/585 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 640/660 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 640/660 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 650/665 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 650/665 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified NIH 3T3-derived EVs were stained with ExoBrite™ APC CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified NIH 3T3-derived EVs were stained with ExoBrite™ APC CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 410/450 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 410/450 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.
SEC-purified NIH 3T3-derived EVs were stained with ExoBrite™ APC CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified NIH 3T3-derived EVs were stained with ExoBrite™ APC CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 650/665 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 650/665 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 640/660 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 640/660 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 560/585 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 560/585 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
SEC-purified mouse NIH 3T3-derived EVs were stained with ExoBrite™ 490/515 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.
SEC-purified mouse NIH 3T3-derived EVs were stained with ExoBrite™ 490/515 CD63 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.

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