ExoBrite™ CD63 Flow Antibody

ExoBrite™ CD63 Flow Antibody

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ExoBrite™ CD63 Flow Antibody
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ R-PE CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
EVs were isolated from MCF-7 cell supernatant with CD9 affinity beads. The bead-bound EVs were stained with ExoBrite™ CD63 490/515 Flow Antibody (green) or ExoBrite™ 490/515 IgG1 Isotype Control Flow Antibody (gray), then analyzed by flow cytometry in the FITC channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ APC CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified HeLa-derived EVs were stained first with ExoBrite™ 560/585 CD63 in 100 uL, followed by 1X ExoBrite™ 650/665 Annexin EV Stain. EVs were detected on a CytoFLEX LX flow cytometer in the PE and APC channels. When we gated on ExoBrite™ 650/665 Annexin-positive particles, ~16% were also positive for CD63.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 650/665 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
ExoBrite™ CD63 Flow Antibody - Image 7
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 560/585 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 490/515 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 410/450 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.

ExoBrite™ CD63 Flow Antibody

ExoBrite™ CD63 Flow Antibody provides bright, low-background detection of EV marker CD63 in purified or bead-bound EVs by flow cytometry.

ExoBrite™ CD63 Flow Antibody

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SKU: P004 Categories: Tags: Brand:

Validated Antibody for Reliable Detection of EV Marker CD63

ExoBrite™ CD63 Flow Antibody is a mouse monoclonal IgG1, kappa antibody validated by Biotium for the optimal detection of CD63, a widely used extracellular vesicle (EV) marker, by flow cytometry.

Designed for both purified and bead-bound EVs, this antibody ensures bright signal, low background, and excellent signal-to-noise performance. With an updated buffer formulation, ExoBrite™ CD63 Flow Antibody offers improved staining specificity and reproducibility across applications.

Available in six colour options (Pacific Blue™, FITC, PE, APC, R-PE, and APC conjugates), ExoBrite™ CD63 enables flexible panel design and can be combined with other EV markers such as CD9 and CD81 for multi-parameter EV analysis.

Key Features

  • Specific detection of EV marker CD63 by flow cytometry
  • Bright fluorescence, low background
  • Validated for purified and bead-bound EVs
  • Six colour options for panel flexibility
  • Updated buffer formulation for enhanced signal-to-noise

Notes: Biotium products are available only in Singapore and Thailand.

 

Detect EVs with confidence using ExoBrite™ CD63 Flow Antibody – validated for purified and bead-bound vesicles.

*Please leave us a message during checkout to indicate which kit you need, and our team will process your order accordingly.

Product Attributes

Attribute Details
Antibody number P004
SwissProt P08962
Antibody type Primary
Clonality Monoclonal
Host species Mouse
Isotype IgG1, kappa
Antibody reactivity (target) CD63
Synonyms gp55; granulophysin; LAMP-3; AD1; MLA1; ME491; NGA; OMA81H; PTLGP40; TSPAN30
Species reactivity Human, Baboon, Cynomolgus monkey, Non-human primates
Human gene symbol CD63
Entrez gene ID 967
Unigene 445570
Molecular weight 26 kDa (core protein); 30–60 kDa (glycosylated)
Target cellular localisation Exosomes/EVs, Lysosomes, Plasma membrane, Multivesicular bodies
Cell/tissue expression Exosomes, Platelets, Granulocytes, Lymphocytes, Monocytes/Macrophages
Verified applications Exosome staining (verified)
Positive control MCF-7 cells, MCF-7 derived exosomes
Recommended concentration 5 µL per 0.1 mL exosomes (flow cytometry)
Research areas Exosomes/EVs
Conjugate formulation Proprietary buffer containing 0.05% sodium azide
Shelf life ≥ 24 months from receipt (if stored as recommended)
Storage conditions Store at 2–8 °C, protect fluorescent conjugates from light
Regulatory status Research Use Only (RUO)
Product origin May contain BSA (from bovine serum) or recombinant BSA (CHO cells). Inquire for lot-specific details.

Variations

Antibody Ex/Em (nm) Target Species Reactivity Detection Channel Catalog No.
ExoBrite™ 410/450 CD63 Flow Antibody 416/452 CD63 Human Pacific Blue™ P004-410
ExoBrite™ 490/515 CD63 Flow Antibody 490/516 CD63 Human FITC P004-490
ExoBrite™ 560/585 CD63 Flow Antibody 562/584 CD63 Human PE P004-560
ExoBrite™ 640/660 CD63 Flow Antibody 642/663 CD63 Human APC P004-640
ExoBrite™ R-PE CD63 Flow Antibody 496/546, 565/578 CD63 Human PE P004-RPE
ExoBrite™ APC CD63 Flow Antibody 651/660 CD63 Human APC P004-APC

Documentation

Proven Performance

SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 490/515 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 490/515 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ R-PE CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ R-PE CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 410/450 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 410/450 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.
EVs were isolated from MCF-7 cell supernatant with CD9 affinity beads. The bead-bound EVs were stained with ExoBrite™ CD63 490/515 Flow Antibody (green) or ExoBrite™ 490/515 IgG1 Isotype Control Flow Antibody (gray), then analyzed by flow cytometry in the FITC channel.
EVs were isolated from MCF-7 cell supernatant with CD9 affinity beads. The bead-bound EVs were stained with ExoBrite™ CD63 490/515 Flow Antibody (green) or ExoBrite™ 490/515 IgG1 Isotype Control Flow Antibody (gray), then analyzed by flow cytometry in the FITC channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ APC CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ APC CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified HeLa-derived EVs were stained first with ExoBrite™ 560/585 CD63 in 100 uL, followed by 1X ExoBrite™ 650/665 Annexin EV Stain. EVs were detected on a CytoFLEX LX flow cytometer in the PE and APC channels. When we gated on ExoBrite™ 650/665 Annexin-positive particles, ~16% were also positive for CD63.
SEC-purified HeLa-derived EVs were stained first with ExoBrite™ 560/585 CD63 in 100 uL, followed by 1X ExoBrite™ 650/665 Annexin EV Stain. EVs were detected on a CytoFLEX LX flow cytometer in the PE and APC channels. When we gated on ExoBrite™ 650/665 Annexin-positive particles, ~16% were also positive for CD63.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 650/665 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 650/665 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 640/660 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 640/660 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 560/585 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 560/585 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.

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