Exo-spin™ technology combines precipitation and size exclusion chromatography (SEC), making it superior to other techniques that rely solely on one method. Using only precipitation for exosome isolation will result in co-purification of large amounts of non-exosomal proteins and other material as well as carryover of the precipitant. SEC is reliable for exosome isolation, but a precipitation step is needed to concentrate your sample prior to SEC isolation. If there is no precipitation, a lower exosome recovery will be observed. We provide a simple two-step protocol which allows you to purify your sample in less than 2 hours with consistent and reliable results!

However, the EX03 Exo-spin™ kit only contains pre-packed and equilibrated ready-to-use SEC columns as the precipitation step might not be recommended for some applications (e.g. mass spectrometry) or not needed for small volumes of sera sample.

The EX03 Exo-spin™ kit has been developed to process any sample smaller than 100 µl. Plasma contains much higher protein levels than sera and can only be processed directly using the EX02 or EX04 Exo-spin™ kit. EX03 Exo-spin™ kit may be used to clean-up samples which have been purified by other methods, such as precipitation and ultracentrifugation, in order to remove proteins and smaller molecules. Exo-spin™ columns are also very effective for the removal of excess dye in fluorescently labelled exosome samples.

Which Exo-spin™ kit shall I choose for my sample volume?

Highly concentrated exosome samples (e.g. 1×10¹² particles/ml) other than blood can also be used.

Exo-spin™ column and resin pore size

The column bed volume is 500 µl, allowing for 100 µl of volume to be loaded on top of the column.

The pore size of the resin is approximatively 30 nm to attain a highly pure exosome elution. All other molecules (e.g. proteins, lipids) which are smaller than 30 nm will enter into the pores and remain trapped in the column.

Highest recovery and purity

As mentioned above, essentially all proteins and lipids will be retained in the column and will elute later than the exosomes, ensuring a highly pure sample ready for your downstream application.

Reproducibility

All our columns are manufactured in our laboratory to ensure a high reproducibility between each lot. As proof of reproducibility and batch-to-batch consistency, a large number of peer-reviewed scientific papers have been published describing the use of Exo-spin™.

Storage

Upon receipt, store purification columns and Exo-spin™ Buffer at 4°C. All other components should be stored at room temperature (15°C – 25°C).

Frequently Asked Questions (FAQs)

For any additional questions, please refer to FAQs document below.

Start today! Select our starter pack

We designed the ideal starter pack to guide your exosome research. The starter pack includes the exosome purification kit of your choice, exosome validated antibodies, and NTA profiling analysis. The complete details can be found in the product page here.

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EX03 Exo-spin™ kit comparative data

NTA measurement of samples prepared from cell culture medium: (a) Exo-spin™ yields significantly higher numbers of vesicles of the expected 40-120 nm size range than alternatives including (b) ultracentrifugation (c) competitor kit. The size distribution profile obtained with Exo-spin™ most closely resembles ultra-centrifugation. Lower rRNA contamination levels Analysis on Bioanalyzer instrument shows RNA preps (Trizol) following (d) Exo-spin™ purification from cell culture samples compared with (e) ultracentrifugation or (f) competitor kit.

Exosomes purified with Exo-spin™ columns primarily elute in the range between 100 μl and 300 μl. Collecting this 200 μl fraction considerably enriches for exosomes over proteins and smaller molecules. Starting sample is 100 μl mouse serum. 

Characterizing Exo-spin™ isolated exosomes using nanoparticle tracking analysis (NTA)

Exosome characterization with NTA. Exosomes have been isolated using the Exo-spin™ kit and analysis performed with the ZetaView® instrument.

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The EX03 Exo-spin™ kit is compatible with all downstream application and has been published in a large range of different applications.

Mass spectrometry:

• Campos A, Salomon C, Bustos R, Díaz J, Martínez S, Silva V, Reyes C, Díaz-Valdivia N, Varas-Godoy M, Lobos-González L and Quest AF. (2018). Caveolin-1-containing extracellular vesicles transport adhesion proteins and promote malignancy in breast cancer cell lines. Nanomedicine 13(20):2597-2609.(4):e0154274.

Exosome labelling:

• Shu SL, Yang Y, Allen CL, Maguire O, Minderman H, Sen A, Ciesielski MJ, Collins KA, Bush PJ, Singh P, Wang X, Morgan M, Qu J, Bankert RB, Whiteside TL, Wu Y and Ernstoff MS. (2018). Metabolic reprogramming of stromal fibroblasts by melanoma exosome microRNA favours a pre-metastatic microenvironment. Scientific Reports 8: 12905.

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[accordion title=”User guide”]EX03_Exo-spin_User_Guide[/accordion]

[accordion title=”FAQs”] FAQs[/accordion]
[accordion title=”MSDS/Tech Sheet”] EX03_and_EX04_MSDS[/accordion]
[accordion title=”Product Brochure”] CellGS exosome brochure.pdf[/accordion]
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