High-Efficiency Nucleic Acid Loading into Extracellular Vesicles for Functional Delivery

The EVfect Transfection Kit is purpose-built for the efficient encapsulation of nucleic acids into pre-purified extracellular vesicles (EVs), enabling targeted delivery without liposomal contamination. Validated across multiple nucleic acid types—including ASOs, siRNA, miRNA, mRNA, and plasmid DNA—this kit supports both in vitro and in vivo applications.

The streamlined 40-minute protocol eliminates the use of liposomal reagents and micelle formation, ensuring clean and reproducible EV re-purification. This guarantees that downstream cellular responses arise solely from EV-mediated delivery, avoiding artefacts from co-purified lipid complexes or residual transfectants.

Key Features & Benefits

• ✅ Broad Compatibility: Works with ASOs, siRNA, miRNA, mRNA, and plasmid DNA

• ✅ High Loading Efficiency: Maximises nucleic acid encapsulation for potent functional delivery

• ✅ Clean Delivery: No liposomes or micelles—ensures EV-only mediated effects

• ✅ Fast & Simple Protocol: Only 40 minutes from start to finish

• ✅ Reproducible Results: Facilitates consistent EV re-purification and downstream analyses

• ✅ Versatile Applications: Suitable for in vitro and in vivo EV delivery studies

  Kit Contents:

• 1 × Dilution Buffer I (5 mL)
• 1 × Reagent M (180 µL)
• 1 × Reagent K (180 µL)
• 1 × Vial lyophilised JOT-RBCEV-A1 (1–1.5 × 10¹¹ particles)

Note: These products are distributed only in Singapore and Thailand.