High-Efficiency, Stable EV Functionalisation for Targeted Delivery and Tracking

The EVcraft Surface Modification Kit leverages advanced bioconjugation chemistry to enable stable, high-density functionalisation of extracellular vesicles (EVs) without altering their size distribution or causing aggregation. Capable of displaying up to 700 antibodies or 60,000 small molecule probes per vesicle, EVcraft offers unmatched flexibility for engineering EVs for targeted delivery, immune evasion, and in vivo tracking.

Compatible with a broad range of functional molecules, the kit empowers researchers to expand EV capabilities for diverse applications, from therapeutic targeting to advanced imaging.

Key Features & Benefits

• ✅ High Loading Capacity: Up to 700 IgGs or 60,000 probes per EV

• ✅ Stable Conjugation: No aggregation or change in polydispersity index

• ✅ Versatile Molecule Attachment: Supports antibodies, nanobodies, ligands, cytokines, anti-phagocytic proteins, fluorescent probes, and reporters

• ✅ Targeted Delivery: Customise EVs for antigen-specific or receptor-specific targeting

• ✅ Enhanced Tracking: Covalently attach fluorescent or reporter probes for accurate in vitro and in vivo imaging

• ✅ Simple Workflow: Includes all reagents and buffer exchange columns for efficient surface modification

Applications:

• Targeted delivery of therapeutic EV cargo
• Immune modulation and receptor engagement
• Delayed or reduced phagocytic clearance
• Live-cell and in vivo EV tracking
• Mechanistic EV research

Kit Contents:

• 4 × vials Probe A
• 4 × vials Probe B
• 8 × buffer exchange columns (with 16 collection tubes)
• 1 mL 10× Reaction Buffer
• 1 mL 10× STOP Buffer

Note: These products are distributed only in Singapore and Thailand.