EMBER™ Ultra RNA Gel Kit

EMBER™ Ultra RNA Gel Kit

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EMBER™ Ultra RNA Gel Kit
The ExoBrite™ EV Total RNA Isolation Kit was used to extract RNA from 6 x 10^10 EVs (derived from Jurkat cells, isolated by SEC) and a total yield of 100 ng RNA was obtained. 10 ng of the EV RNA was analyzed by gel electrophoresis using the EMBER™ Ultra RNA Gel Kit with a 1.5% gel. The gel was imaged using the Gel-Bright™ Laser Diode Gel Imager. L = Low Range ssRNA Ladder (NEB).
Comparison of EMBER™ Ultra RNA Gel (left) with 1% agarose gel post-stained with SYBR® Gold. NEB ssRNA Ladder or total Jurkat cell RNA were denatured at 70°C for 10 minutes in EMBER™ Ultra RNA Loading Dye (for EMBER™ gel) or NEB RNA Loading Dye (for SYBR® Gel) and run on 1% agarose/1X TBE gels. EMBER® Ultra Gel was imaged immediately after electrophoresis, while the other gel was post-stained in 1X SYBR® Gold/1X TBE for 30 minutes. Gels were imaged on Biotium's Gel-Bright™ Laser Diode Gel Illuminator. The EMBER Ultra® RNA Gel Kit offers comparable or superior sensitivity and resolution compared to SYBR® Gold, without a time-consuming post-staining step.
Five-fold dilutions of ssRNA Ladder from NEB (200 ng to 12.5 ng per lane) were separated on a 1% agarose/1X TBE gel using the EMBER™ Ultra RNA Gel Kit. The gel was imaged on Biotium's Gel-Bright™ Laser Diode Gel Illuminator, the Safe Imager™ 2.0 Blue-Light Transilluminator (Thermo Fisher), or a UV transilluminator with SYBR® Filter. They EMBER™ Ultra gel system is optimized for Gel-Bright™ Laser Diode and blue light illuminators, but also can be used with UV illuminators with lower sensitivity.

EMBER™ Ultra RNA Gel Kit

The EMBER™ Ultra RNA Gel Kit provides unmatched sensitivity for RNA electrophoresis, detecting as little as ≤5 ng RNA without post-staining steps.

EMBER™ Ultra RNA Gel Kit

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SKU: 41044-T, 41044 Categories: Tags: Brand:

The Most Sensitive RNA Gel Stain in a Convenient Precoated Agarose Format

RNA analysis demands maximum sensitivity to detect low-abundance transcripts while ensuring RNA integrity. The EMBER™ Ultra RNA Gel Kit delivers the highest sensitivity available for RNA gel electrophoresis in a fast, simple, and convenient format.

Using EMBER™ Ultra Precoated Agarose and a specially formulated RNA Loading Dye, this kit allows direct denaturing, loading, and imaging without the need for post-electrophoresis staining. Bands can be visualised immediately, saving time while producing sharper and more sensitive results compared to conventional RNA stains.

With the ability to detect as little as ≤5 ng RNA, EMBER™ Ultra outperforms ethidium bromide (EtBr) and other fluorescent dyes. It also detects DNA contamination in RNA samples, making it an excellent quality control tool for RNA integrity assessment.

Key Features

  • Unmatched sensitivity: Detect as little as ≤5 ng RNA.
  • Fast workflow: No post-staining or special gel system required.
  • Convenient format: Precoated agarose gels simplify preparation.
  • RNA integrity check: Evaluate total RNA quality and DNA contamination.
  • Broad compatibility: Optimal for blue LED gel imagers; also compatible with UV imagers.
  • Flexible gel casting: Works with agarose concentrations from 1% to 5% for different RNA sizes.
Five-fold dilutions of ssRNA Ladder from NEB (200 ng to 12.5 ng per lane) were separated on a 1% agarose/1X TBE gel using the EMBER™ Ultra RNA Gel Kit. The gel was imaged on Biotium's Gel-Bright™ Laser Diode Gel Illuminator, the Safe Imager™ 2.0 Blue-Light Transilluminator (Thermo Fisher), or a UV transilluminator with SYBR® Filter. They EMBER™ Ultra gel system is optimized for Gel-Bright™ Laser Diode and blue light illuminators, but also can be used with UV illuminators with lower sensitivity.
Five-fold dilutions of ssRNA Ladder from NEB (200 ng to 12.5 ng per lane) were separated on a 1% agarose/1X TBE gel using the EMBER™ Ultra RNA Gel Kit. The gel was imaged on Biotium’s Gel-Bright™ Laser Diode Gel Illuminator, the Safe Imager™ 2.0 Blue-Light Transilluminator (Thermo Fisher), or a UV transilluminator with SYBR® Filter. They EMBER™ Ultra gel system is optimized for Gel-Bright™ Laser Diode and blue light illuminators, but also can be used with UV illuminators with lower sensitivity.
Comparison of EMBER™ Ultra RNA Gel (left) with 1% agarose gel post-stained with SYBR® Gold. NEB ssRNA Ladder or total Jurkat cell RNA were denatured at 70°C for 10 minutes in EMBER™ Ultra RNA Loading Dye (for EMBER™ gel) or NEB RNA Loading Dye (for SYBR® Gel) and run on 1% agarose/1X TBE gels. EMBER® Ultra Gel was imaged immediately after electrophoresis, while the other gel was post-stained in 1X SYBR® Gold/1X TBE for 30 minutes. Gels were imaged on Biotium's Gel-Bright™ Laser Diode Gel Illuminator. The EMBER Ultra® RNA Gel Kit offers comparable or superior sensitivity and resolution compared to SYBR® Gold, without a time-consuming post-staining step.
Comparison of EMBER™ Ultra RNA Gel (left) with 1% agarose gel post-stained with SYBR® Gold. NEB ssRNA Ladder or total Jurkat cell RNA were denatured at 70°C for 10 minutes in EMBER™ Ultra RNA Loading Dye (for EMBER™ gel) or NEB RNA Loading Dye (for SYBR® Gel) and run on 1% agarose/1X TBE gels. EMBER® Ultra Gel was imaged immediately after electrophoresis, while the other gel was post-stained in 1X SYBR® Gold/1X TBE for 30 minutes. Gels were imaged on Biotium’s Gel-Bright™ Laser Diode Gel Illuminator. The EMBER Ultra® RNA Gel Kit offers comparable or superior sensitivity and resolution compared to SYBR® Gold, without a time-consuming post-staining step.
The ExoBrite™ EV Total RNA Isolation Kit was used to extract RNA from 6 x 10^10 EVs (derived from Jurkat cells, isolated by SEC) and a total yield of 100 ng RNA was obtained. 10 ng of the EV RNA was analyzed by gel electrophoresis using the EMBER™ Ultra RNA Gel Kit with a 1.5% gel. The gel was imaged using the Gel-Bright™ Laser Diode Gel Imager. L = Low Range ssRNA Ladder (NEB).
The ExoBrite™ EV Total RNA Isolation Kit was used to extract RNA from 6 x 10^10 EVs (derived from Jurkat cells, isolated by SEC) and a total yield of 100 ng RNA was obtained. 10 ng of the EV RNA was analyzed by gel electrophoresis using the EMBER™ Ultra RNA Gel Kit with a 1.5% gel. The gel was imaged using the Gel-Bright™ Laser Diode Gel Imager. L = Low Range ssRNA Ladder (NEB).

Applications

  • RNA integrity analysis
  • Detection of RNA contamination or degradation
  • Assessment of DNA contamination in RNA preps
  • RNA quality control for qPCR, RNA-seq, microarray, and IVT workflows

✅ For research use only. Not for use in diagnostic procedures.

*Biotium Products are only available in Singapore and Thailand.

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