Antibodies, Conjugates & Labelling, Cell imaging & Analysis, Secondary Antibodies
Aging & Neuroscience, Cell & Organelle Stains, Cell imaging & Analysis, Cell Surface & Membrane Dyes, Cytoskeletal Stains, in vivo Cell Imaging Dyes, Neuroscience Cell Dyes
Antibodies, Conjugates & Labelling, Cell imaging & Analysis, Secondary Antibodies
Aging & Neuroscience, Cell & Organelle Stains, Cell imaging & Analysis, Cell Surface & Membrane Dyes, Cytoskeletal Stains, in vivo Cell Imaging Dyes, Neuroscience Cell Dyes
$420.00
CryoEase-PF is the only dual temperature compatibility, DMSO-free, protein-free cryopreservation medium proven to maintain >85% MSC viability post-thaw with uncompromised expansion potential and phenotype, meeting ISCT criteria for clinical-grade MSCs. This media is developed by leading cell therapy developers in Singapore for global cell therapy developers for allogeneic/autologous MSC production, global trials, and biobanking.
1. Clinically Superior Viability & Recovery
✅ >85% Viability After 72h at 4°C (vs. <75% in competing formulations)
✅ 23-Hour Population Doubling Time (PDT) post-thaw – near-identical to fresh MSCs
✅ 30-Hour PDT Even After 72h Hypothermic Storage – ensures expandability after shipping delays
Validation Data:
2. Unmatched Functional Integrity
✔ Preserved Trilineage Differentiation (Osteogenic, Chondrogenic, Adipogenic)
✔ Stable Surface Marker Profile – Critical for IND/EMA filings
✔ Consistent Performance Across Batches – Pre-mixed, endotoxin-tested, GMP-compliant
3. Global Logistics Simplified
| Parameter | CryoEase-PF | Commercial Alternatives |
| 72h Post-Thaw Viability | >85% | <75% |
| Population Doubling Time | 23h (acute thaw), 30h (72h delay) | 35–50h (variable) |
| ISCT Compliance | Full retention of phenotype & differentiation | Often compromised |
| Toxicity Risks | DMSO-free, xeno-free | DMSO-dependent |
Now Expanding to New Cell Types – Join Our Validation Network
We Invite Researchers to Collaborate!
Are you working with sensitive cell lines that challenge conventional cryopreservation? Partner with us to:
✓ Test CryoEase-PF in your specific application
✓ Access complimentary samples for pilot studies
✓ Explore co-development opportunities
Be among the first to test CryoEase-PF on your specialized cell lines – Contact our team for collaborative opportunities or sample requests!
Email: [email protected]
| Volume | 100ml |
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To demonstrate the versatility of CryoEase-DF, we stored human adipose-derived MSCs at a density of 5 x 106 cells/mL. Upon thawing, the cells were stored at 4°C for up to 72 hours. Cell viability was assessed by 7AAD staining. Cells stored in CryoEase-PF retained >85% viability after 72 hours as compared to other commercially available formulations with <75% viability.
It is critical that the cells are not just viable but remain expandable in culture. MSCs frozen in CryoEase-PF were thawed and plated immediately (Acute Thaw) or stored at 4°C for up to 72 hours before being plated at 5,000 cells/cm2. The population doubling time (PDT) was determined after 4 days. Remarkably, cells stored in CryoEase-PF retain a PDT of approx. 23 hours and 30 hours when plated immediately or stored for 72 hours.
To ensure that MSC identity is not compromised post-storage, MSCs were subjected to phenotyping in accordance with the criteria set forth by the International Society for Cell and Gene Therapy (ISCT) (Dominici et. al.). MSC stored in CryoEase-PF retained both their surface marker profile and trilineage differentiation potential.
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