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CryoEase-PF | DMSO & Protein Free Cryoperservation Media

CryoEase-PF | DMSO & Protein Free Cryoperservation Media

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Product image of CryoEase-PF with text highlighting DMSO-free, protein-free formula, GMP-compliant materials, dual-temperature compatibility, and reduced reliance on dry ice or nitrogen.
CryoEase-PF | DMSO & Protein Free Cryoperservation Media - Image 2
Promotional image of CryoEase-PF bottle with text stating over 85% viability of MSCs after 72 hours at 4°C, emphasizing preservation of stem cell identity and function post-thaw.
Image showing CryoEase-PF bottle with DMSO-free and protein-free tags, designed for dual-temperature compatibility and developed by stem cell researchers for bench-to-bedside use.
Text introduction to AGEMbio's mission and how CryoEase-PF preserves next-gen MSCs for cancer therapy; includes bottle image and background of pipette in lab.
Graph comparing MSC viability between CryoEase-PF and commercial alternatives after 72 hours. Additional bar graph shows better doubling time, highlighting robust cell performance post-thaw.
Microscopic images showing successful adipogenic, osteogenic, and chondrogenic differentiation in MSCs stored using CryoEase-PF, both freshly thawed and after 72 hours.
Flow cytometry data and table confirming high positivity of MSC markers (CD73, CD90, CD105) and low expression of negative markers, supporting MSC identity preservation.
Comparison table illustrating how CryoEase-PF outperforms commercial alternatives in cell viability, doubling time, toxicity risk, and ISCT compliance for stem cell preservation.
Infographic showing CryoEase-PF applications in clinical settings, biobanking, research laboratories, and veterinary medicine, supporting predictable outcomes and high-quality stem cell experiments.
Product information table for CryoEase-PF, a DMSO-free and protein-free cryopreservation media. Includes product name (CryoEase-PF), catalogue number (AS-9012-1), size (100 mL), and storage conditions (store at 4–8°C). Note mentions a 12-month shelf life from the date of manufacture.
CryoEase-PF: DMSO-Free and Protein-Free Cell Transport & Freezing Media

CryoEase-PF | DMSO & Protein Free Cryoperservation Media

Chemically defined, DMSO-free and animal component-free medium optimized for superior MSC viability and function.

Product image of CryoEase-PF with text highlighting DMSO-free, protein-free formula, GMP-compliant materials, dual-temperature compatibility, and reduced reliance on dry ice or nitrogen.

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SKU: AS-9012 Categories: Tags: Brand:

CryoEase-PF – A DMSO-Free, Protein-Free Cryopreservation Media for MSCs & Sensitive Cells

Dual-Temperature Compatible. Validated Performance. Translationally Ready.

CryoEase-PF is a next-generation, DMSO-free and protein-free cryopreservation medium designed specifically for the long-term preservation and short-term stability of human mesenchymal stem cells (MSCs) and other sensitive cell types.

What sets CryoEase-PF apart is its dual-temperature compatibility—the only cryomedia designed for both long-term storage in vapor-phase liquid nitrogen (-150°C) and short-term stability at 4°C for up to 72 hours. This breakthrough formulation enables real-world transport, decentralized trial execution, and cold chain simplification, ensuring your cells remain viable and functional — from cryobank to bedside.
Engineered in Singapore by AGEMBio, a cell therapy innovator, CryoEase-PF is optimized to meet the demands of biobanking, global cell therapy trials, and translational research workflows.

With >85% post-thaw viability and full ISCT-compliant phenotype retention even after 72 hours of hypothermic storage, CryoEase-PF provides unmatched protection for MSCs—from cryobank to bedside.

 

Validated Performance

  • >85% Viability After 72 Hours at 4°C
    Outperforms commercial cryomedia with <75% viability in the same conditions
  • 23-Hour Population Doubling Time Post-Thaw
    Comparable to fresh MSCs
  • 30-Hour PDT After 72h Hypothermic Hold
    Ensures reliable cell expansion after transportation delays
  • ISCT-Compliant MSC Identity
    Retains CD73+/CD90+/CD105+ and CD34-/CD45-/HLA-DR- phenotype

Validation conducted using human adipose-derived MSCs (5 x 10⁶ cells/mL) stored at -150°C and held at 4°C for 72h post-thaw. Viability assessed via 7AAD staining.

 

What sets CryoEase-PF apart is its dual-temperature compatibility—the only cryomedia designed for both long-term storage in vapor-phase liquid nitrogen (-150°C) and short-term stability at 4°C for up to 72 hours. Dual-Temperature Compatibility: Real-World Ready

CryoEase-PF is uniquely optimized for translational logistics and multi-site trial workflows, allowing:

  • Long-Term Biobanking: Vapor-phase LN₂ storage (-150°C)
  • Short-Term Transport Stability: Maintains viability at 4°C for 72h
  • Cold Chain Flexibility: Reduce reliance of dry ice and liquid nitrogen
  • Allogeneic & Autologous Ready: Supports scalable, GMP workflows

This dual-temperature stability is unmatched in DMSO-free, protein-free cryopreservation — making CryoEase-PF the only truly translational-ready solution on the market.

 

Engineered for Translational Success

CryoEase-PF is formulated to support both allogeneic and autologous MSC workflows, ensuring functional and phenotypic consistency critical to IND filings, EMA submissions, and global trial logistics.

  • Trilineage Differentiation Maintained – Osteogenic, chondrogenic, adipogenic
  • Batch-to-Batch Consistency – Pre-mixed, endotoxin-tested
  • No DMSO, No Proteins, No Risk – Eliminates toxicity concerns from cryoprotectants

Built for Global Deployment

Dual-temperature compatibility enables CryoEase-PF to simplify global logistics:

  • Long-term: Storage in vapor-phase LN₂ (-150°C)
  • Short-term: Viability at 4°C for up to 72 hours — ideal for multi-site advanced research studies and regional cell transport

 

CryoEase-PF vs. Conventional Cryomedia

Parameter CryoEase-PF Typical Commercial Media
72h Viability at 4°C >85% <75%
Post-Thaw PDT 23h (acute), 30h (72h hold) 35–50h (variable)
ISCT Compliance ✅ Full retention ❌ Often compromised
Cryoprotectant Risk DMSO-free, xeno-free DMSO-dependent

 

Applications in MSC-Based Therapies

CryoEase-PF is engineered to meet the needs of every frontline advancing stem cell innovation:

  • Clinical Settings:
    Support MSC-based therapies with predictable and reproducible outcomes, critical for ensuring patient safety and therapeutic success.
  • Biobanking:
    Preserve high-quality stem cells for long-term storage while maintaining viability and potency, ensuring reliable downstream applications.
  • Research Laboratories:
    Enable robust, repeatable experiments by safeguarding stem cell integrity during cryopreservation and recovery.
  • Veterinary Medicine:
    Facilitate advanced stem cell applications in animal health with DMSO-free, protein-free preservation optimized for sensitive cell types.

Developed by AGEMBio – Distributed by Atlantis Bioscience

CryoEase-PF is developed by AGEMBio, an NUS spin-off backed by SGInnovate, with a mission to redefine stem cell therapy through
immune-activating MSC platforms.

As exclusive distributor, Atlantis Bioscience supports the deployment of CryoEase-PF across APAC and beyond—aligning with our vision to deliver bench-to-bedside solutions that enable life-saving research, clinical translation, and scalable biomanufacturing.

 

Collaborate With Us – Expand CryoEase-PF to Your Cell Type

We’re inviting researchers and developers worldwide to explore CryoEase-PF’s capabilities across new cell types:

  • ✓ Pilot studies with complimentary samples
  • ✓ Collaborative method development
  • ✓ Custom formulation support for sensitive cell lines

 

Contact us to join the validation network:

email: [email protected]
website: www.atlantisbioscience.com/contact-us/

Form

Liquid
Brand

Atlantis Bioscience (Powered by AGEMBio)
Country of Origin

Singapore
Storage Condition

4-8ºC
Quality Control

Certificate of analysis for each batch will be provided upon request
Volume

100ml

Supporting Data

To demonstrate the versatility of CryoEase-DF, we stored human adipose-derived MSCs at a density of 5 x 106 cells/mL. Upon thawing, the cells were stored at 4°C for up to 72 hours. Cell viability was assessed by 7AAD staining. Cells stored in CryoEase-PF retained >85% viability after 72 hours as compared to other commercially available formulations with <75% viability.

 

MSC cell viability data stored in CryoEase-PF for 72 hours.

 

It is critical that the cells are not just viable but remain expandable in culture. MSCs frozen in CryoEase-PF were thawed and plated immediately (Acute Thaw) or stored at 4°C for up to 72 hours before being plated at 5,000 cells/cm2. The population doubling time (PDT) was determined after 4 days. Remarkably, cells stored in CryoEase-PF retain a PDT of approx. 23 hours and 30 hours when plated immediately or stored for 72 hours.

 

Cell viability data of MSC cells stores in CryoEase-PF after plating.

 

To ensure that MSC identity is not compromised post-storage, MSCs were subjected to phenotyping in accordance with the criteria set forth by the International Society for Cell and Gene Therapy (ISCT) (Dominici et. al.). MSC stored in CryoEase-PF retained both their surface marker profile and trilineage differentiation potential.

 

ISCT standard of MSCs cells stored in CryoEase-PF

Cell image of MSC stored in CryoEase-PF for 72 hours vs acute thaw

Protocol

Cryopreservation Protocol

  1. Trypsinize cells from a monolayer.
  2. Quantify cell number and viability.
  3. Pellet cells by centrifugation.
  4. Resuspend in an appropriate amount of CryoEase-PF.
    1. We recommend a freezing density of 1-5 x 10⁶ cells/mL
  5. Transfer to cryovials.
  6. Equilibrate at room temperature for 10-15 mins.
  7. Store at -80C.
    1. Optional: use control rate freezer/freezing devices for -1C/min freezing

 

Post-Thaw Sample Protocol

  1. Thaw vials in 37 °C water bath (or use automated thawing device – eg, ThawStar)
  2. Add complete media/appropriate thawing buffer at a 1:5 ratio (eg, 4 mL media to 1 mL frozen thawed cell suspension)
  3. Quantify cell number and viability.
  4. The sample is ready for downstream use. No spinning is required.

Documentation

Find supporting information and directions for use in the Product Information Booklet below.

MSC Workflow Bundle

Expand, Transport & Preserve

Atlantis Bioscience, in partnership with AGEMBio, offers a complete MSC solution that supports every step of your workflow — from high-efficiency cell expansion to safe short-term transport and long-term cryopreservation.

STEMGOLD – High-Performance hMSC Growth Medium Kit

  • Accelerates MSC growth with faster doubling times
  • Supports robust expansion of bone marrow, umbilical cord, and adipose-derived MSCs
  • Preserves trilineage differentiation (osteoblast, chondrocyte, adipocyte)
  • Eliminates pre-coating
  • Ensure ISCT compliance

→ Learn more about STEMGOLD

HypoGuard – The Global Standard for Hypothermic Cell Transport

  • Preserves >95% MSC viability for 5 days at 2–8°C — no cryogenic shipping required
  • Serum/Xeno & Protein-Free
  • Suitable for sensitive cells (e.g. MSCs, PBMCs, etc.)
  • Mysoplasma & Endotoxin Tested
  • Ready to use – no special equipment or freezing required

→ Learn more about HypoGuard

Bundle These Products for Seamless MSC Research

From expansion (STEMGOLD) to transport (HypoGuard) to cryopreservation (CryoEase-PF), we provide a complete, integrated solution to support your translational MSC workflow — whether you’re running preclinical trials, global studies, or biobanking.

 

Ready to streamline your MSC process? Contact our team to discuss bundle pricing or sample requests.

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