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CF® Dye Tyramide

CF® Dye Tyramide

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CF® Dye Tyramide for TSA, bright fluorescent alternative to Opal and Aluora for multiplex IHC and spatial biology.
Illustration of the tyramide signal amplification system. A cell or tissue sample is labeled with primary and secondary antibody using conventional methods. The horseradish peroxidase, conjugated to the secondary antibody, catalyzes the conversion of labeled tyramide into a reactive radical. The tyramide radical then covalently binds to nearby tyrosine residues, providing high-density labeling
Sequential multiplex tyramide labeling of human colon FFPE section with three CF® Dye Tyramides. Cytokeratin (pan) was labeled with CF®488A Tyramide (green); Histone H1 was labeled with Cyanine 555 Tyramide (red); ZO1 was labeled with CF®640R Tyramide (magenta).
Human tonsil FFPE section stained with anti-CD68 (C68/684+KP1) and goat anti-mouse HRP, detected using CF®488A tyramide in Tyramide Amplification Buffer Plus.
CF® Dye Tyramide - Image 5
Human tonsil FFPE sections heated in AntiFix™ antigen retrieval buffer, then stained with anti-Ki67 MKI67/2466, goat anti-mouse HRP, and CF®488A tyramide using Tyramide Amplification Buffer Plus.
Section of rat brain and choroid plexus stained with mouse anti-GFAP GA5 antibody, HRP goat anti-mouse, and CF®740 Tyramide using Biotium's Tyramide Amplification Buffer Plus (glial cells, blue). Blood vessels were stained with CF®488A LEL Tomato Lectin (green), and nuclei were stained with NucSpot® 568/580 Nuclear Stain (red). Imaged on a LI-COR® Odyssey® M Imaging System in the 488, 520, and 800 channels.
PFA-fixed HeLa cells stained with WGA-HRP detected with CF®740 Tyramide (magenta) in Biotium's Tyramide Amplification Buffer Plus. Nuclei were stained with Hoehcst (cyan). Imaged on an Evident FV4000 confocal imaging system with 730 nm laser line.
CF®740 free acid or Alexa Fluor® 750 free acid was diluted to 2 uM in 1X Tyramide Amplification Buffer with 0.0015% hydrogen peroxide and incubated at room temperature, protected from light. Dye concentration was measured by absorbance at the time points shown. CF®740 remained stable in the presence of hydrogen peroxide over 24 hours (≤90% of the starting dye concentration), while Alexa Fluor® 750 degraded to less than 10% of the starting concentration after 24 hours.
Sequential labeling of formaldehyde-fixed HeLa cells with two Tyramide Amplification Kits. Mitochondria (green) were imaged with rabbit anti-COXIV primary antibody and Tyramide Amplification Kit with HRP goat anti-rabbit IgG and CF®488A-tyramide, followed by peroxidase quenching. Nucleoli (magenta) were visualized with mouse anti-cyclin B1 primary antibody and Tyramide Amplification Kit with HRP Goat anti-mouse IgG and CF®568-tyramide. Actin (red) was detected with CF®640R-phalloidin, and cell nuclei (blue) were stained with DAPI.
2uM of CF405S-tyramide (left) and 10uM of CF750-tyramide (right) staining human colon FFPE section labeled with mouse anti-panCK primary antibody and HRP-Goat anti-Mouse secondary antibody. The staining is performed using our Tyramide Enhancer Buffer.
ZO-1 staining in human colon cryosections detected using CF®488A, Cyanine 555 (Cy®3), CF®640R, and CF®680R tyramide with Biotium's Tyramide Amplification Buffer Plus.

CF® Dye Tyramide

CF® Dye Tyramides enable high-sensitivity Tyramide Signal Amplification (TSA) for multiplex IHC, ICC and ISH. Choose from 22 bright, photostable dyes — ideal alternatives to Opal®, Aluora® and AF680/AF660 reagents.

CF® Dye Tyramide for TSA, bright fluorescent alternative to Opal and Aluora for multiplex IHC and spatial biology.

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SKU: 92170, 92197, 96057, 92198, 96053, 92171, 92199, 96066, 92172, 96077, 96021, 92173, 96085, 92174, 92194, 92175, 96022, 92195, 92196, 96127, 96128, 96124, 96052, 96090 Categories: Tags: Brand:

High-Sensitivity Fluorescent Tyramides for Multiplex IHC, ICC, ISH & CycIF

Boost immunofluorescence sensitivity by up to 100-fold with CF® Dye Tyramides for Tyramide Signal Amplification (TSA).

Designed for multiplex immunohistochemistry (IHC), immunocytochemistry (ICC), in situ hybridization (ISH), and cyclic immunofluorescence (CycIF), CF® Dye Tyramides enable high-density, covalent in situ labeling of low-abundance protein and nucleic acid targets — even in autofluorescent human FFPE tissues.

A powerful alternative to Opal®, Aluora®, Alexa Fluor® and other TSA systems.

 

Why Choose CF® Dye Tyramides?

★ Up to 100× Higher Detection Sensitivity

• Detect low-expression biomarkers
• Overcome tissue autofluorescence
• Reduce primary antibody concentration
• Improve signal-to-noise ratio

★ 22 Bright & Photostable Colors

Wide spectral coverage from UV to near-IR for multiplex panel design.
Water-soluble, photostable, and optimized for fluorescence imaging systems.

★ Multiplex Without Host Species Limitation

Covalent deposition allows multiple sequential rounds of amplification (CycIF compatible).

 

Near-IR Optimisation for Spatial Biology Platforms

For automated staining systems (e.g., BOND RX) and spatial biology workflows:

Recommended Near-IR Options

➤ CF®680R Tyramide
• Comparable to AF680, AF660
• Alternative to Opal® 690
• Alternative to Aluora® 700
• Narrow emission profile reduces spillover

➤ CF®740 Tyramide (Recommended for automation)
• Stable in oxidizing TSA buffer
• Better choice than CF®750 and CF®754 for automated platforms
• Reduced spectral spillover

CF®740 free acid or Alexa Fluor® 750 free acid was diluted to 2 uM in 1X Tyramide Amplification Buffer with 0.0015% hydrogen peroxide and incubated at room temperature, protected from light. Dye concentration was measured by absorbance at the time points shown. CF®740 remained stable in the presence of hydrogen peroxide over 24 hours (≤90% of the starting dye concentration), while Alexa Fluor® 750 degraded to less than 10% of the starting concentration after 24 hours.
CF®740 free acid or Alexa Fluor® 750 free acid was diluted to 2 uM in 1X Tyramide Amplification Buffer with 0.0015% hydrogen peroxide and incubated at room temperature, protected from light. Dye concentration was measured by absorbance at the time points shown. CF®740 remained stable in the presence of hydrogen peroxide over 24 hours (≤90% of the starting dye concentration), while Alexa Fluor® 750 degraded to less than 10% of the starting concentration after 24 hours.

➤ CF®754 Tyramide
• Alternative to Opal® 780
• Alternative to Aluora® 750
• Broad absorption profile — consider panel design carefully

⚠ Note: CF®750 Tyramide is unstable in oxidizing TSA buffer and should be added immediately before use.

 

Applications

✔ Multiplex IHC
✔ ICC
✔ ISH / FISH
✔ CycIF / t-CycIF
✔ Human FFPE tissue
✔ Low-abundance target detection
✔ Akoya PhenoImager workflows
✔ Spatial biology panel development

 

Also Available

• HRP Goat Anti-Rabbit TSA Kits
• HRP Streptavidin TSA Kits
• Ready-to-Use Amplification Buffer
• Tyramide Amplification Buffer Plus
• TyraMax™ Amplification Dyes for next-generation TSA performance

 

Upgrade to TyraMax™

Looking for even brighter signal and improved buffer stability?
Explore TyraMax™ Amplification Dyes, Biotium’s next-generation tyramide dyes offering enhanced brightness, photostability and working solution stability compared to original CF® Dye Tyramides and other TSA systems.

For a deeper understanding of panel design, workflow optimisation and multiplex strategy, read our practical guide on TSA-Based Multiplex Immunofluorescence in Spatial Biology here: https://www.atlantisbioscience.com/blog/tsa-multiplex-immunofluorescence-spatial-biology/

 

Note: Biotium products are distributed only in Singapore and Thailand.

Variations

Product Ex/Em (nm) MW (g/mol) Size Catalog No.
CF®350 Tyramide 347/448 ~614 0.5 mg 92170
CF®405S Tyramide 404/431 ~689 0.5 mg 92197
CF®405M Tyramide 408/452 ~621 0.5 mg 96057
CF®405L Tyramide 395/545 ~1692 0.5 mg 92198
CF®430 Tyramide 426/498 ~707 0.5 mg 96053
CF®488A Tyramide 490/515 ~666 0.5 mg 92171
CF®514 Tyramide 516/548 ~1337 0.5 mg 92199
CF®532 Tyramide 527/558 ~804 0.5 mg 96066
CF®543 Tyramide 541/560 ~1006 0.5 mg 92172
CF®550R Tyramide 551/577 ~806 0.5 mg 96077
CF®555 Tyramide 555/565 ~1120 0.5 mg 96021
CF®568 Tyramide 562/583 ~833 0.5 mg 92173
CF®583R Tyramide 586/609 ~892 0.5 mg 96085
CF®594 Tyramide 593/614 ~848 0.5 mg 92174
CF®620R Tyramide 617/639 ~857 0.5 mg 92194
CF®640R Tyramide 642/662 ~951 0.5 mg 92175
CF®647 Tyramide 650/665 ~1104 0.5 mg 96022
CF®660R Tyramide 663/682 ~1007 0.5 mg 92195
CF®680R Tyramide 680/701 ~1031 0.5 mg 92196
CF®710 Tyramide 712/736 ~977 0.5 mg 96127
CF®725 Tyramide 729/750 ~1005 0.5 mg 96128
CF®740 Tyramide 742/767 ~1005 0.5 mg 96124
CF®750 Tyramide* 755/779 ~3040 0.5 mg 96052
CF®754 Tyramide 748/793 ~1000 0.5 mg 96090

* CF®750 Tyramide is not stable in TSA buffer and should be added immediately before staining.

Product Attributes

Product Attributes
Size 0.5 mg
Chemical Reactivity (reacts with) Tyrosine residues
Functional Group Tyramide
Assay Type / Options Tissue staining
Storage Conditions Store at -10 to -35 °C. Protect from light.
Detection Method / Readout Fluorescence microscopy

Documentation

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