Keratinocyte Differentiation Media-suitable for 2D, 3D and co-cultures
Keratinocyte Differentiation Media-suitable for 2D, 3D and co-cultures
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A chemically-defined serum-free media suitable for 3D organotypic Skin culture, 3D Keratinocyte collagen/airlift culture to allow for proper stratification of primary and immortalised keratinocyte.
It supports both the growth and differentiation of keratinocyte from the start of the culturing procedure to the end of the experimentation. This medium is also suitable to support the growth of skin cells embedded in Extracellular matrix such as fibroblast in collagen matrix.
Key Features
- Suitable for 2D ,3D and co-cultures . One solution fits all chemically defined, serum-free
- Allows differentiation of fibroblasts, keratinocytes, melanocytes and mesenchymal stem cells
- Allow proper stratification of primary & immortalised keratinocytes
- Can be used for 3D skin culture from start to the end (no different media required)
- Support growth of skin cells embedded in extracellular matrix
Applications
- Skin Organoid Culture
- Epidermal Differentiation
- Dermal Substitute
- 3D bioprinting of skin equivalent
- Skin Microfluidic system
Testimonials
Keratinocyte Differentiation Media (AS-9010) Used For 3D In-vitro Skin
The EU has banned animal testing for cosmetics and their ingredients since 2013. A growing demand for in vitro skin models has been rising steadily to cater for both the safety testing (Skin irritation) and efficacy testing (functional test).
In the experiment, primary keratinocytes were seeded on a cultured insert to form a confluent layer. The confluent layer of cells is lifted to an air-liquid interface for 14 days to stimulates the keratinocytes to differentiate (stratification) and form a multi-layered epidermis that contains stratum corneum layers. The specialized medium primarily used consists of DMEM and Ham’s F12 in a specific ratio. It is chemically defined and serum-free to ensure a high reproducibility in result. Treatments can be topically applied to the skin model for a period of times. Histology such as H&E staining and other skin markers are commonly carried out. Gene expression and protein study are also alternative analysis method.
Materials:
- Biowest Trypsin 0.25 % in PBS w/o Calcium w/o Magnesium w/o Phenol Red ( Cat no: L0931-100 )
- Biowest DMEM Low Glucose w/ L-Glutamine w/ Sodium Pyruvate (Cat no: L0060-500)
- Biowest HAM’s F12 Glucose w/ L-Glutamine (Cat no: L0135-500)
- Atlantis Bioscience Keratinocyte Differentiation Media (Cat no: AS-9010)
