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TyraMax™ Amplification Dyes and Kits

TyraMax™ Amplification Dyes and Kits

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TyraMax™ Amplification Dyes and Kits product image
Multicolour fluorescence images of cells stained with TyraMax™ amplification dyes across 12 channels from blue to near-infrared, demonstrating bright, uniform signal and compatibility with high-plex spatial biology and multiplex imaging workflows.
Absorption and emission spectra of TyraMax™ tyramide amplification dyes spanning blue to near-infrared wavelengths, illustrating broad spectral coverage and suitability for multiplex and spectral imaging applications.
Side-by-side fluorescence images and quantitative plots comparing TyraMax™ 740/770 and Aluora™ 750, demonstrating higher photostability and superior stability of TyraMax™ in amplification buffer over 24 hours.
Comparison of near-infrared tyramide amplification dyes showing brighter membrane staining and higher photostability for TyraMax™ 660/680 and 680/700 compared to Opal™ Polaris 690 and Aluora™ 700, with fluorescence intensity retained over time under continuous illumination.

TyraMax™ Amplification Dyes and Kits

TyraMax™ Amplification Dyes are next-generation TSA reagents delivering brighter, more photostable signals and improved workflow stability for multiplex IHC, ICC, FISH, and spatial biology applications.

TyraMax™ Amplification Dyes and Kits product image

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SKU: 96134, 96135, 96136, 96137, 96138, 96139, 96140, 96141, 96142, 96143, 96144, 96145, 33029, 33030, 33031 Category: Tags: Brand:

Next-Generation Tyramide Amplification Dyes for Brighter, More Stable Multiplex Spatial Biology

TyraMax™ Amplification Dyes are Biotium’s next-generation tyramide signal amplification (TSA) reagents, engineered to meet the growing demands of spatial biology and high-plex imaging. Compared to earlier CF® Dye tyramides and other TSA dyes on the market, TyraMax™ offers clear advantages in signal brightness, photostability, and working solution stability.

Designed for applications such as immunohistochemistry (IHC), immunocytochemistry (ICC), and in situ hybridization (FISH), TyraMax™ dyes deliver robust performance in both manual and automated staining workflows. Notably, TyraMax™ dyes remain stable in amplification buffer for at least 24 hours, including near-infrared dyes—an important advantage for high-throughput and automated platforms.

Key Features

  • Largest selection of tyramide colours available, with options from blue to near-infrared
  • Brighter and more photostable signals compared to other TSA reagents
  • Stable in amplification buffer for ≥24 hours, including near-IR dyes
  • Efficient reagent usage: stain up to 100 samples at 1:100 dilution
  • Simplified workflow: TyraMax™ 740/770 enables single-step detection, reducing hands-on time versus multi-step approaches
  • Flexible formats: available as standalone dyes (with trial sizes), dye sets, and multiplex samplers

 

Power Your Multiplex Panels with TyraMax™

Tyramide signal amplification (TSA), also known as Catalyzed Reporter Deposition (CARD), is a highly sensitive method for detecting low-abundance targets. Its compatibility with cyclic immunofluorescence (CycIF) has made TSA indispensable for modern multiplex and spatial biology workflows.

TyraMax™ dyes were developed to push TSA performance further, delivering brighter and more photostable signals than Aluora® and Opal® reagents, while also offering improved chemical stability. This makes TyraMax™ particularly well suited for high-plex panel development, spectral imaging, and long acquisition times.

Comparison of near-infrared tyramide amplification dyes showing brighter membrane staining and higher photostability for TyraMax™ 660/680 and 680/700 compared to Opal™ Polaris 690 and Aluora™ 700, with fluorescence intensity retained over time under continuous illumination.
Biotium’s far-red/near-IR TyraMax® Amplification Dyes offer advantages in brightness and photostability compared to other TSA dyes. PFA-fixed HeLa cells stained with WGA-HRP detected with TyraMax™, Opal™ Polaris, or Aluora™ Spatial dye. Imaged using a 40X oil objective on Evident FV4000 confocal system with the indicated laser line. Photostability measurements were done on an Olympus IX71 epifluorescence microscope using a Cy®5 filter cube. Stained cells were imaged every 10 seconds for 120 seconds, and mean fluorescence was normalized to the first image taken.
Side-by-side fluorescence images and quantitative plots comparing TyraMax™ 740/770 and Aluora™ 750, demonstrating higher photostability and superior stability of TyraMax™ in amplification buffer over 24 hours.
TyraMax™ 740/770 has excellent brightness, photostabilty, and working solution stability, making it compatible with automated workflows. PFA-fixed HeLa cells stained with WGA-HRP detected with TyraMax™ 740/770 Amplification Dye or Aluora™ 750 Spatial dye and imaged in PBS using a 40X oil objective on Evident FV4000 confocal system with 730 nm laser in the Alexa Fluor® 700 detection channel. For photostability measurement, images were collected every second during continuous illumination at 10% laser power. Mean fluorescence at each time point was normalized to the fluorescence for the first image captured. For working solution stability testing, OD-matched solutions of TyraMax™ 740/770 and Aluora™ 750 dyes were prepared in Tyramide Amplification Buffer Plus with 0.0015% hydrogen peroxide. Absorbance was measured on an Agilent Cary 60 spectrophotometer, and then the solutions were left at room temperature, protected from light, and absorbance was measured at various time points up to 24 hours. TyraMax™ 740/770 was stable in the presence of hydrogen peroxide over 24 hours, while Aluora™ 750 degraded almost completely.

TyraMax vs Opal vs Aluora

Feature TyraMax™ Opal™ Aluora™
Signal performance Brighter, more photostable Dimmer, rapid fade Dimmer and weaker signal
Working stability Stable in buffer for ≥24 hours Limited stability Can be unstable (e.g. Aluora™ 750 degrades overnight)
Colour selection 12 colours (blue to near IR) 8 colours 8 colours

For optimal performance, TyraMax™ dyes are recommended for use with Tyramide Amplification Buffer Plus (Cat. No. 22029).

Formats Available

  • Standalone TyraMax™ dyes (including trial sizes)
  • 3-colour and 5-colour dye sets with DAPI counterstain
  • Sampler kits for custom multiplex panel optimisation

These flexible options support both conventional fluorescence and spectral imaging workflows, enabling researchers to design and scale multiplex panels with confidence.

Applications

  • Multiplex immunohistochemistry (IHC)
  • Immunocytochemistry (ICC)
  • Fluorescence in situ hybridization (FISH)
  • Cyclic immunofluorescence (CycIF)
  • Spatial biology and tissue profiling

 

Note: Biotium products is only available in Singapore, Thailand and Malaysia.

Functional group

Tyramide
Chemical reactivity (reacts with)

Tyrosine residues
Assay type/options

Tissue staining
Detection method/readout

Fluorescence microscopy
Storage Conditions

Store at 2 to 8 °C, Protect from light

Variations

TyraMax™ Amplification Dyes

Dye Abs/Em (nm) Laser Line Detection Channel Dye Features Size Catalog No.
TyraMax™ 410/450 408/452 405 nm DAPI / Alexa Fluor® 405 Unique tyramide color, spectrally similar to Alexa Fluor® 405 20 µL 96134-20UL
100 µL 96134-100UL
TyraMax™ 430/500 421/497 405 nm FITC Brighter than Aluora® 430 and Opal® 480 20 µL 96135-20UL
100 µL 96135-100UL
TyraMax™ 400/550 394/547 405 nm FITC Unique tyramide color, spectrally similar to Pacific Orange® 20 µL 96136-20UL
100 µL 96136-100UL
TyraMax™ 490/520 497/518 488 nm FITC Brighter than Opal® 520, replacement for Aluora® 488 20 µL 96137-20UL
100 µL 96137-100UL
TyraMax™ 555/565 594/569 555 nm or 561 nm TRITC Brighter than Opal® 570, replacement for Aluora® 555 20 µL 96138-20UL
100 µL 96138-100UL
TyraMax™ 560/580 562/584 555 nm or 561 nm TRITC Alternative to Aluora® 555, Opal® 570 with superior photostability 20 µL 96139-20UL
100 µL 96139-100UL
TyraMax™ 630/650 631/650 633 nm or 640 nm Cy®5 Bright and photostable alternative to Aluora® 647, Opal® 650 20 µL 96140-20UL
100 µL 96140-100UL
TyraMax™ 647/670 650/670 633 nm or 640 nm Cy®5 Brighter than Opal® 650, replacement for Aluora® 647 20 µL 96141-20UL
100 µL 96141-100UL
TyraMax™ 660/680 663/683 633 nm or 640 nm Alexa Fluor® 680 Unique tyramide color, spectrally similar to Alexa Fluor® 680. Brighter and more photostable than Opal® 690 when excited at 640 nm 20 µL 96142-20UL
100 µL 96142-100UL
TyraMax™ 680/700 680/701 685 nm (detectable with 640 nm excitation) Alexa Fluor® 680 Brighter and more photostable than Opal® 690 when excited at 685 nm 20 µL 96143-20UL
100 µL 96143-100UL
TyraMax™ 710/740 711/736 685 nm Alexa Fluor® 700 Brighter and more photostable than Aluora® 700 20 µL 96144-20UL
100 µL 96144-100UL
TyraMax™ 740/770 742/768 730 nm Alexa Fluor® 750 Single-step detection, unlike Opal® 780. Stable in amplification buffer for up to 24 hours, unlike Aluora® 750 20 µL 96145-20UL
100 µL 96145-100UL

 

TyraMax™ 3-Plex Amplification Dye Set with DAPI (Cat. No. 33029)

Component # Component Name Size
96137-100UL TyraMax™ 490/520 Amplification Dye, 100X 100 µL
96139-100UL TyraMax™ 560/580 Amplification Dye, 100X 100 µL
96140-100UL TyraMax™ 630/650 Amplification Dye, 100X 100 µL
99897-50UL DAPI, 1000X 50 µL

 

TyraMax™ 5-Plex Amplification Dye Set with DAPI (Cat. No. 33030)

Component # Component Name Size
96137-100UL TyraMax™ 490/520 Amplification Dye, 100X 100 µL
96139-100UL TyraMax™ 560/580 Amplification Dye, 100X 100 µL
96140-100UL TyraMax™ 630/650 Amplification Dye, 100X 100 µL
96143-100UL TyraMax™ 680/700 Amplification Dye, 100X 100 µL
96145-100UL TyraMax™ 740/770 Amplification Dye, 100X 100 µL
99897-50UL DAPI, 1000X 50 µL

TyraMax™ Amplification Dye Spectral Sampler (Cat. No. 33031)

Component # Component Name Size
96134-20UL TyraMax™ 410/450 20 µL
96135-20UL TyraMax™ 430/500 20 µL
96136-20UL TyraMax™ 400/550 20 µL
96137-20UL TyraMax™ 490/520 20 µL
96138-20UL TyraMax™ 555/565 20 µL
96139-20UL TyraMax™ 560/580 20 µL
96140-20UL TyraMax™ 630/650 20 µL
96141-20UL TyraMax™ 647/670 20 µL
96142-20UL TyraMax™ 660/680 20 µL
96143-20UL TyraMax™ 680/700 20 µL
96144-20UL TyraMax™ 710/740 20 µL
96145-20UL TyraMax™ 740/770 20 µL

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