Plasmid DNA Production & Manufacturing Services

Atlantis Bioscience, the authorised partner of PackGene, provides researchers and biotech companies with comprehensive plasmid DNA production and manufacturing solutions — from research-grade plasmids to full GMP production.

Plasmids are produced by growing bacterial cells containing the desired DNA sequence, followed by extraction and purification. Through PackGene’s Plasmid Extraction service, Atlantis Bioscience delivers high-quality plasmids for transfection and preclinical research. With years of process optimisation, PackGene ensures consistent yield and purity — from 100 µg to 1000 mg and beyond, with high homogeneity and ultra-low endotoxin levels (<10 EU/mg) suitable for research and clinical applications.

Our integrated platform supports every stage of your workflow, from vector design to large-scale clinical production, ensuring purity, consistency, and regulatory compliance.

Service Overview

Plasmid Preparation

Flexible scaling | Sequence fidelity | Rapid turnaround
PackGene’s plasmid preparation service supports lab-scale DNA production with guaranteed sequence accuracy and low endotoxin content.
Ideal for early-stage discovery, vector construction, and functional studies.

Features:

• Fast delivery  for all drug development stages
• Flexible sizes from 100µg to 2g (preclinical) and up to 100g (GMP)
• Customizable QC (restriction enzyme digestion, sequencing, purity testing)
• Compatible with AAV, LNP, CAR-T, and vaccine applications
  

Research Grade Plasmid Preparation

High-quality plasmids for discovery research and preclinical validation

Produced under stringent quality control, research-grade plasmids are ideal for in vitro transfection, viral vector packaging, and early functional studies.

Features:

• Flexible scale from 10 µg to 1000 mg with high homogeneity
• Low endotoxin levels for biosafety
• Suitable for transfection, viral production, CAR-T, and more

Preclinical Plasmid DNA

Bridging discovery to IND submission

Preclinical-grade plasmids are manufactured with enhanced traceability, animal-free production, and comprehensive quality documentation suitable for regulatory submissions.

Highlights:

• Endotoxin-free plasmids with optional animal-free processing
• Suitable for in vivo studies from mice to NHP
• Available in quantities from 10 mg up to 100g

GMP Plasmid

Clinical-grade, DMF-registered plasmid DNA for global regulatory submission

PackGene’s GMP plasmid manufacturing adheres to ISO 13485 and GMP standards and is Drug Master File (DMF) registered, providing global regulatory support for gene and cell therapy programs.

Key Features:

• cGMP plasmid production up to 200L with flexible scales
• Strict contamination control using isolated lines and single-use tech
• Complete batch documentation and CoA provided
• Residual host DNA, protein, sterility, and identity testing included
• Suitable for IND filing, clinical trials, and commercial manufacturing
• Over 10 years of expert manufacturing with full process support

• Key benefits

• Service Details

• Performance

Productivity

Two independent cGMP production lines supporting up to 200 L production scale.

Quality

Isolated production lines, rigorous cleaning procedures, and disposable materials ensure complete prevention of cross-contamination.

Flexibility

Multiple production scales and grades enable high-efficiency project completion at a reasonable cost.

Expertise

PackGene’s experienced production team brings over 10 years of expertise in plasmid manufacturing.

Advanced Equipment

Single-use technology is applied across upstream and downstream processes, with Fill & Finish performed under VHP isolator conditions.

Scalability

Our efforts to optimize the plasmid prep process have resulted in increased efficiency and scalability that drive consistent batch to batch yields with regard to both fermentation and purification recovery rates.

Batch-to-batch consistency

The examples below demonstrate our fermentation yield consistency across three separate fermentation rounds using the same AAV cis plasmid template in a 50L fermenter.

State-of-the-Art Plasmid DNA Manufacturing Facility

As the authorised partner of PackGene, Atlantis Bioscience provides access to world-class plasmid DNA production and GMP manufacturing facilities that meet the highest global quality standards.

The 11,000-square-foot GMP facility, equipped with 5 L to 200 L fermenters, supports both process development (PD) and large-scale GMP plasmid production. With an annual capacity exceeding 1,000 g of GMP-grade plasmid DNA, PackGene enables reliable, high-yield manufacturing for AAV vector, mRNA vaccine, and gene therapy applications.

A dedicated team of over 40 experts with decades of experience in AAV plasmid preparation ensures round-the-clock operations and consistent quality. Together, Atlantis Bioscience and PackGene empower researchers in Singapore and Southeast Asia to accelerate translational research from bench to bedside.

Applications of Plasmid DNA Manufacturing

Our high-quality plasmid DNA supports a wide range of biomedical and translational research applications, providing reliable performance across discovery, preclinical, and GMP stages.

Key applications include:

• AAV and LNP vector manufacturing for efficient gene delivery and therapeutic development
• mRNA template generation for vaccine and RNA-based drug production
• CAR-T and gene therapy manufacturing, enabling consistent and scalable plasmid supply
• CRISPR/Cas9 delivery systems for precise genome editing research
• Vaccine and antibody development, from early R&D to large-scale validation

Real-World Example of Translational Support

At Atlantis Bioscience, we bridge research and clinical application by supporting translational teams with GMP-like plasmid sourcing, mRNA, and LNP services. We recently helped a spin-off from a leading research institution obtain a GMP-like plasmid for their clinical trial application, ensuring quality, compliance, and timely delivery.

Through our partnership with PackGene Biotech, a global CRO and CDMO specialising in AAV vectors, mRNA, plasmid DNA, and lentiviral solutions, we provide integrated support across drug delivery, vaccine development, and viral vector manufacturing. From early discovery to cell and gene therapy trials, we enable seamless translation from bench to bedside.

Partner with Atlantis Bioscience

Atlantis Bioscience proudly serves as PackGene’s authorized partner, bridging global plasmid manufacturing excellence with local research expertise.
We support academic, biotech, and translational teams through every stage — from small-scale plasmid prep to GMP-grade, DMF-registered manufacturing.

Get in touch to discuss your plasmid production needs or request a quotation tailored to your workflow.