ExoBrite™ STORM CTB EV Staining Kits

ExoBrite™ STORM CTB EV Staining Kits

ExoBrite™ STORM CTB EV Staining Kits deliver bright, low-background EV staining for dSTORM, using CTB conjugates with validated CF® Dyes.

ExoBrite™ STORM CTB EV Staining Kits cover image

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Description

Product Description

CTB Conjugates with CF® Dyes for Super-Resolution dSTORM Imaging of EVs

ExoBrite™ STORM CTB EV Staining Kits are fluorescent cholera toxin subunit B (CTB) conjugates optimised for direct stochastic optical reconstruction microscopy (dSTORM) imaging of extracellular vesicles (EVs).

CTB binds with high affinity to GM1 gangliosides present in lipid rafts and EV membranes, making it a reliable probe for visualising EVs. Unlike traditional lipophilic dyes, ExoBrite™ STORM CTB stains show minimal background aggregation, ensuring accurate identification of purified or bead-bound EVs.

Incorporating CF® Dyes validated for STORM, these stains provide bright signal, low background, and superior single-molecule resolution. They are fully compatible with antibody co-staining, enabling multi-parameter EV characterisation at the nanoscale.

Key Features

  • Optimally formulated CTB conjugates for EV staining
  • Designed for STORM/dSTORM super-resolution imaging
  • Bright signal, low background for single-molecule accuracy
  • Suitable for purified or bead-bound EVs
  • Compatible with antibody co-staining
  • Available in four colours (CF®505, CF®583R, CF®647, CF®680)

 

Kit Components

  • ExoBrite™ STORM CTB EV Stain
  • ExoBrite™ Reconstitution Solution (makes 100 µL of 500X staining solution)

 

Validated EV Sources

EV Source Biotium Data Customer Reported
A549 cells High
CHO cells Low
hASC Low
HEK293T cells High
HeLa cells Low
HUVEC cells Low
J774 cells High
Jurkat cells High
MCF-7 cells High
Plasma Low
Raji cells High
Skeletal myoblasts High
U2OS cells Low
U937 cells Low

 

Notes: Biotium products are available only in Singapore and Thailand.

 

Achieve nanoscale resolution in EV imaging with ExoBrite™ STORM CTB EV Staining Kits – powered by CF® Dyes for unmatched dSTORM performance.

*Please leave us a message during checkout to indicate which kit you need, and our team will process your order accordingly.

Variations

Variations

Product Ex/Em (nm) Laser Line(s) Detection Channel Size Options Catalog No.
ExoBrite™ STORM CF®505 CTB EV Staining Kit 505/519 488 FITC 100 / 500 Labelings 30115-T / 30115
ExoBrite™ STORM CF®583R CTB EV Staining Kit 583/609 555 or 561 Rhodamine, Texas Red® 100 / 500 Labelings 30116-T / 30116
ExoBrite™ STORM CF®647 CTB EV Staining Kit 652/668 633–640 Cy®5 100 / 500 Labelings 30117-T / 30117
ExoBrite™ STORM CF®680 CTB EV Staining Kit 681/698 633–640 Cy®5.5 100 / 500 Labelings 30118-T / 30118
Documentation

Documentation

Proven Performance

Proven Performance

SEC purified MCF-7-derived EVs stained with ExoBrite™ STORM CF®680 CTB EV Stain (left) compared to the same stain in buffer (right). EVs were detected on a CytoFLEX LX flow cytometer in the R712 channel.
SEC purified MCF-7-derived EVs stained with ExoBrite™ STORM CF®680 CTB EV Stain (left) compared to the same stain in buffer (right). EVs were detected on a CytoFLEX LX flow cytometer in the R712 channel.
SEC purified MCF-7-derived EVs stained with ExoBrite™ STORM CF®647 CTB EV Stain (left) compared to the same stain in buffer (right). EVs were detected on a CytoFLEX LX flow cytometer in the APC channel.
SEC purified MCF-7-derived EVs stained with ExoBrite™ STORM CF®647 CTB EV Stain (left) compared to the same stain in buffer (right). EVs were detected on a CytoFLEX LX flow cytometer in the APC channel.
SEC purified MCF-7-derived EVs stained with ExoBrite™ STORM CF®583R CTB EV Stain (left) compared to the same stain in buffer (right). EVs were detected on a CytoFLEX LX flow cytometer in the PE channel.
SEC purified MCF-7-derived EVs stained with ExoBrite™ STORM CF®583R CTB EV Stain (left) compared to the same stain in buffer (right). EVs were detected on a CytoFLEX LX flow cytometer in the PE channel.
SEC purified MCF-7-derived EVs stained with ExoBrite™ STORM CF®505 CTB EV Stain (left) compared to the same stain in buffer (right). EVs were detected on a CytoFLEX LX flow cytometer in the FITC channel.
SEC purified MCF-7-derived EVs stained with ExoBrite™ STORM CF®505 CTB EV Stain (left) compared to the same stain in buffer (right). EVs were detected on a CytoFLEX LX flow cytometer in the FITC channel.
Super-resolution dSTORM images of human colorectal cancer cell line derived EVs. EVs were stained with anti-tetraspanin antibodies (an anti-CD9/CD63/CD81 cocktail, all conjugated to CF®647, shown in magenta) together with 1X ExoBrite™ 560/585 (in cyan). Samples were prepared using ONI's EV Profiler Kit and acquired using the Nanoimager S Mark II from ONI (Oxford Nanoimaging, UK). Data was processed using a beta-release version of CODI, ONI's cloud-based data analysis platform. Scale bars are 500 nm (zoomed out, left panel) and 50 nm for single-EV panels (right). Image courtesy of ONI.
Super-resolution dSTORM images of human colorectal cancer cell line derived EVs. EVs were stained with anti-tetraspanin antibodies (an anti-CD9/CD63/CD81 cocktail, all conjugated to CF®647, shown in magenta) together with 1X ExoBrite™ 560/585 (in cyan). Samples were prepared using ONI’s EV Profiler Kit and acquired using the Nanoimager S Mark II from ONI (Oxford Nanoimaging, UK). Data was processed using a beta-release version of CODI, ONI’s cloud-based data analysis platform. Scale bars are 500 nm (zoomed out, left panel) and 50 nm for single-EV panels (right). Image courtesy of ONI.