ExoBrite™ IgG1 Isotype Control Flow Antibody

ExoBrite™ IgG1 Isotype Control Flow Antibody

ExoBrite™ IgG1 Isotype Control Flow Antibody provides a non-reactive negative control for CD9, CD63, and CD81 antibodies in EV flow cytometry.

ExoBrite™ IgG1 Isotype Control Flow Antibody cover image

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Non-Reactive Negative Control for ExoBrite™ Flow Antibodies

ExoBrite™ IgG1 Isotype Control Flow Antibody is a mouse monoclonal IgG1, kappa antibody validated as a negative control for ExoBrite™ Flow Antibodies when detecting extracellular vesicles (EVs) by flow cytometry.

This antibody is non-reactive with human cells and matches the isotype of ExoBrite™ antibodies against CD9, CD63, and CD81, ensuring accurate assessment of background signal. By providing a baseline for non-specific binding, ExoBrite™ IgG1 Isotype Control helps researchers validate staining specificity in EV studies.

Available in five colour options (Pacific Blue™, FITC, PE, R-PE, and APC), it is designed to complement the full ExoBrite™ tetraspanin antibody portfolio.

Key Features

  • Non-reactive negative control for ExoBrite™ Flow Antibodies
  • Matches isotype of CD9, CD63, and CD81 antibodies
  • Ensures accurate baseline for background binding
  • Available in five colour options
  • Essential for validating flow cytometry staining specificity

 

EVs were isolated from MCF-7 cell supernatant with CD9 affinity beads. The bead-bound EVs were stained with ExoBrite™ CD9 490/515 Flow Antibody (green) or ExoBrite™ 490/515 IgG1 Isotype Control Flow Antibody (gray), then analyzed by flow cytometry in the FITC channel.
EVs were isolated from MCF-7 cell supernatant with CD9 affinity beads. The bead-bound EVs were stained with ExoBrite™ CD9 490/515 Flow Antibody (green) or ExoBrite™ 490/515 IgG1 Isotype Control Flow Antibody (gray), then analyzed by flow cytometry in the FITC channel.

Notes: Biotium products are available only in Singapore and Thailand.

 

Strengthen your EV analysis with ExoBrite™ IgG1 Isotype Control Flow Antibody – the essential negative control for reliable results.

*Please leave us a message during checkout to indicate which kit you need, and our team will process your order accordingly.

Product Attributes

Attribute Details
Antibody number P008
Antibody type Isotype Control
Clonality Monoclonal
Host species Mouse
Isotype IgG1, kappa
Antibody reactivity (target) Isotype Control (non-reactive in human cells)
Validated in protein array Monospecific
Recommended concentration 5 µL per 0.1 mL exosomes (flow cytometry)
Conjugate formulation Proprietary buffer containing 0.05% sodium azide
Shelf life ≥ 24 months from receipt (if stored as recommended)
Storage conditions Store at 2–8 °C, protect fluorescent conjugates from light
Shipping condition Room temperature
Regulatory status Research Use Only (RUO)
Product origin May contain BSA (from bovine serum) or recombinant BSA (CHO cells). Inquire for lot-specific details.

Variations

Antibody Ex/Em (nm) Target Species Reactivity Detection Channel Catalog No.
ExoBrite™ 410/450 IgG1 Isotype Control Flow Antibody 416/452 Isotype Control None Pacific Blue™ P008-410
ExoBrite™ 490/515 IgG1 Isotype Control Flow Antibody 490/516 Isotype Control None FITC P008-490
ExoBrite™ 560/585 IgG1 Isotype Control Flow Antibody 562/584 Isotype Control None PE P008-560
ExoBrite™ R-PE IgG1 Isotype Control Flow Antibody 496/546, 565/578 Isotype Control None PE P008-RPE
ExoBrite™ 640/660 IgG1 Isotype Control Flow Antibody 642/663 Isotype Control None APC P008-640

Documentation