ExoBrite™ CD9 (Mouse) Flow Antibody cover image

SEC-purified MCF-7-derived EVs were stained with ExoBrite™ R-PE CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.

EVs were isolated from MCF-7 cell supernatant with CD9 affinity beads. The bead-bound EVs were stained with ExoBrite™ CD63 490/515 Flow Antibody (green) or ExoBrite™ 490/515 IgG1 Isotype Control Flow Antibody (gray), then analyzed by flow cytometry in the FITC channel.

SEC-purified MCF-7-derived EVs were stained with ExoBrite™ APC CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.

SEC-purified HeLa-derived EVs were stained first with ExoBrite™ 560/585 CD63 in 100 uL, followed by 1X ExoBrite™ 650/665 Annexin EV Stain. EVs were detected on a CytoFLEX LX flow cytometer in the PE and APC channels. When we gated on ExoBrite™ 650/665 Annexin-positive particles, ~16% were also positive for CD63.

SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 650/665 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.

SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 560/585 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.

SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 490/515 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.

SEC-purified MCF-7-derived EVs were stained with ExoBrite™ 410/450 CD63 Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.

ExoBrite™ CD63 Flow Antibody

ExoBrite™ CD63 Flow Antibody provides bright, low-background detection of EV marker CD63 in purified or bead-bound EVs by flow cytometry.

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Description

Product Description

Validated Antibody for Reliable Detection of Mouse EV Marker CD63

ExoBrite™ CD63 Flow Antibody is a mouse monoclonal IgG1, kappa antibody validated by Biotium for the optimal detection of CD63, a widely used extracellular vesicle (EV) marker, by flow cytometry.

Designed for both purified and bead-bound EVs, this antibody ensures bright signal, low background, and excellent signal-to-noise performance. With an updated buffer formulation, ExoBrite™ CD63 Flow Antibody offers improved staining specificity and reproducibility across applications.

Available in six colour options (Pacific Blue™, FITC, PE, APC, R-PE, and APC conjugates), ExoBrite™ CD63 enables flexible panel design and can be combined with other EV markers such as CD9 and CD81 for multi-parameter EV analysis.

Key Features

Specific detection of EV marker CD63 by flow cytometry
Bright fluorescence, low background
Validated for purified and bead-bound EVs
Six colour options for panel flexibility
Updated buffer formulation for enhanced signal-to-noise

Notes: Biotium products are available only in Singapore and Thailand.

Detect EVs with confidence using ExoBrite™ CD63 Flow Antibody – validated for purified and bead-bound vesicles.

*Please leave us a message during checkout to indicate which kit you need, and our team will process your order accordingly.

Product Attributes

Attribute	Details
Antibody number	P004
SwissProt	P08962
Antibody type	Primary
Clonality	Monoclonal
Host species	Mouse
Isotype	IgG1, kappa
Antibody reactivity (target)	CD63
Synonyms	gp55; granulophysin; LAMP-3; AD1; MLA1; ME491; NGA; OMA81H; PTLGP40; TSPAN30
Species reactivity	Human, Baboon, Cynomolgus monkey, Non-human primates
Human gene symbol	CD63
Entrez gene ID	967
Unigene	445570
Molecular weight	26 kDa (core protein); 30–60 kDa (glycosylated)
Target cellular localisation	Exosomes/EVs, Lysosomes, Plasma membrane, Multivesicular bodies
Cell/tissue expression	Exosomes, Platelets, Granulocytes, Lymphocytes, Monocytes/Macrophages
Verified applications	Exosome staining (verified)
Positive control	MCF-7 cells, MCF-7 derived exosomes
Recommended concentration	5 µL per 0.1 mL exosomes (flow cytometry)
Research areas	Exosomes/EVs
Conjugate formulation	Proprietary buffer containing 0.05% sodium azide
Shelf life	≥ 24 months from receipt (if stored as recommended)
Storage conditions	Store at 2–8 °C, protect fluorescent conjugates from light
Regulatory status	Research Use Only (RUO)
Product origin	May contain BSA (from bovine serum) or recombinant BSA (CHO cells). Inquire for lot-specific details.

Variations

Antibody	Ex/Em (nm)	Target	Species Reactivity	Detection Channel	Catalog No.
ExoBrite™ 410/450 CD63 Flow Antibody	416/452	CD63	Human	Pacific Blue™	P004-410
ExoBrite™ 490/515 CD63 Flow Antibody	490/516	CD63	Human	FITC	P004-490
ExoBrite™ 560/585 CD63 Flow Antibody	562/584	CD63	Human	PE	P004-560
ExoBrite™ 640/660 CD63 Flow Antibody	642/663	CD63	Human	APC	P004-640
ExoBrite™ R-PE CD63 Flow Antibody	496/546, 565/578	CD63	Human	PE	P004-RPE
ExoBrite™ APC CD63 Flow Antibody	651/660	CD63	Human	APC	P004-APC