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ExoBrite™ CD9 (Mouse) Flow Antibody

ExoBrite™ CD9 (Mouse) Flow Antibody

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ExoBrite™ CD9 (Mouse) Flow Antibody cover image
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 410/450 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ APC CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 650/665 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 640/660 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 560/585 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 490/515 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.

ExoBrite™ CD9 (Mouse) Flow Antibody

ExoBrite™ CD9 (Mouse) Flow Antibody provides bright, low-background detection of mouse CD9 in purified or bead-bound EVs by flow cytometry.

ExoBrite™ CD9 (Mouse) Flow Antibody cover image

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SKU: P018 Categories: Tags: Brand:

Validated Antibody for Reliable Detection of Mouse EV Marker CD9

ExoBrite™ CD9 (Mouse) Flow Antibody is a rat monoclonal IgG2a, kappa antibody validated by Biotium for optimal detection of mouse CD9, a key extracellular vesicle (EV) marker, by flow cytometry.

Designed for use with purified and bead-bound EVs, this antibody provides bright signal, low background, and excellent signal-to-noise performance. With an updated buffer formulation, ExoBrite™ CD9 (Mouse) Flow Antibody offers improved staining specificity and reliability.

Available in five colour options (Pacific Blue™, FITC, PE, APC, and APC-R), this antibody supports flexible multi-parameter EV analysis and can be combined with other EV markers such as CD63 and CD81.

Key Features

  • Specific detection of mouse CD9 EV marker by flow cytometry
  • Bright fluorescence, low background
  • Validated for purified and bead-bound EVs
  • Five colour options for flexible panel design
  • Updated buffer formulation for improved signal-to-noise

Notes: Biotium products are available only in Singapore and Thailand.

 

Detect mouse EVs with confidence using ExoBrite™ CD9 (Mouse) Flow Antibody – validated for purified and bead-bound vesicles.

*Please leave us a message during checkout to indicate which kit you need, and our team will process your order accordingly.

Product Attributes

Attribute Details
Antibody number P018
SwissProt P40240
Antibody type Primary
Clonality Monoclonal
Host species Rat (Rattus norvegicus)
Isotype IgG2a, kappa
Antibody reactivity (target) CD9
Synonyms DRAP-27, MRP-1, p-24
Species reactivity Mouse
Human gene symbol CD9
Entrez gene ID 12527
Molecular weight 24 kDa
Target cellular localisation Exosomes/EVs, Plasma membrane
Cell/tissue expression Exosomes, Platelets, Basophils, Eosinophils, Epithelial cells, Lymphocytes
Verified applications Exosome staining (verified)
Positive control RAW 264.7 cells
Recommended concentration 5 µL per 0.1 mL exosomes (flow cytometry)
Research areas Exosomes/EVs
Conjugate formulation Proprietary buffer containing 0.05% sodium azide
Shelf life ≥ 24 months from receipt (if stored as recommended)
Storage conditions Store at 2–8 °C, protect fluorescent conjugates from light
Regulatory status Research Use Only (RUO)
Product origin May contain BSA (from bovine serum) or recombinant BSA (CHO cells). Inquire for lot-specific details.

Variations

Antibody Ex/Em (nm) Target Host Species Detection Channel Catalog No.
ExoBrite™ 410/450 CD9 (Mouse) Flow Antibody 416/452 CD9 Rat (IgG2a, κ) Pacific Blue™ P018-410
ExoBrite™ 490/515 CD9 (Mouse) Flow Antibody 490/516 CD9 Rat (IgG2a, κ) FITC P018-490
ExoBrite™ 560/585 CD9 (Mouse) Flow Antibody 562/584 CD9 Rat (IgG2a, κ) PE P018-560
ExoBrite™ 640/660 CD9 (Mouse) Flow Antibody 642/663 CD9 Rat (IgG2a, κ) APC P018-640
ExoBrite™ APC CD9 (Mouse) Flow Antibody 651/660 CD9 Rat (IgG2a, κ) APC P018-APC

 

Available Sizes

  • 25 tests (catalogue no. suffix -125)
  • 100 tests (catalogue no. suffix -500)

Documentation

Proven Performance

SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 410/450 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 410/450 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the V450 (Pacific Blue) channel.

 

SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ APC CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ APC CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.

 

SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 650/665 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 650/665 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.

 

SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 640/660 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.
SEC-purified RAW 264.7-derived EVs were stained with ExoBrite™ 640/660 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the R660 (APC) channel.

 

SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 560/585 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 560/585 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the Y585 (PE) channel.

 

SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 490/515 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.
SEC-purified mouse RAW 264.7-derived EVs were stained with ExoBrite™ 490/515 CD9 (mouse) Flow Antibody (left). Specific staining was seen, compared with the same antibody in buffer (right). Exosomes were detected on a CytoFLEX LX flow cytometer in the B525 (FITC) channel.

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