ExoBrite™ WGA EV Staining Kits

Sensitive Stains for Purified or Bead-Bound EVs with Broad Compatibility

ExoBrite™ WGA EV Staining Kits are fluorescent conjugates of wheat germ agglutinin (WGA), specifically optimised for bright and sensitive staining of extracellular vesicles (EVs). Unlike hydrophobic membrane dyes that can aggregate or bind non-specifically, ExoBrite™ WGA EV Stains are formulated for clear, low-background staining in flow cytometry.

Validated on EVs derived from 9 cell lines, these stains are compatible with both purified EVs and bead-bound EVs, offering broad coverage across different EV sources. Each kit includes the ExoBrite™ WGA EV Stain and 1X PBS solution, ensuring convenient and reliable experimental use.

By binding to glycoproteins on EV membranes, ExoBrite™ WGA stains provide bright signal intensity and are fully compatible with antibody co-staining (e.g. CD9, CD63, CD81), enabling multi-parameter EV analysis.

Key Features

Optimally formulated WGA conjugates for EV staining
Compatible with both purified and bead-bound EVs
Bright signal, low background, ideal for flow cytometry
Broad compatibility – strong staining across 9 validated EV sources
Compatible with antibody co-staining for multiplex analysis
Available in four colours

SEC-purified EVs were stained with ExoBrite™ WGA EV Stains. MCF-7 derived EVs were stained with ExoBrite™ 410/450, ExoBrite™ 490/515, or ExoBrite™ 560/585. CHO derived EVs were stained with ExoBrite™ 640/660. 410/450 was detected in the Pacific Blue channel, 490/515 was detected in the FITC channel, 560/585 was detected in the PE channel, and 640/660 was detected in the APC channel.

ExoBrite™ 490/515 WGA EV Stain was used to stain either SEC-purified, Jurkat-derived EVs, or artificial liposomes. Robust staining was seen with the EVs, but the liposomes were not stained. The liposomes in this experiment were prepared from Presome® ACD-1, and were verified to be of similar size and concentration to the EVs using the lipophilic dye di-8-ANNEPS (data not shown). Analysis was performed on a CytoFLEX flow cytometer with SSC detected from the 405 nm laser, and ExoBrite™ detected in the FITC channel. Presome® is a registered trademark of NIPPON FINE CHEMICAL CO., LTD.

SEC-purified MCF-7-derived EVs were stained first with ExoBrite™ 490/515 CD81 in 100 uL, followed by 1X ExoBrite™ 640/660 WGA EV Stain. EVs were detected on a CytoFLEX LX flow cytometer in the FITC and APC channels. When we gated on ExoBrite™ 640/660 WGA-positive particles, ~60% were also positive for CD81.

Kit Components

ExoBrite™ WGA EV Stain
ExoBrite™ 1X PBS Solution

Notes

ExoBrite™ WGA EV Stains were validated on 9 cell line-derived EVs but may not stain EVs from all sources.
For ExoBrite™ 490/515 dye, a biotin blocking step is recommended when using streptavidin-coated beads or surfaces.
Biotium products are available only in Singapore and Thailand.

Explore ExoBrite™ WGA EV Staining Kits today – four colours are ready for your EV research.

*Please leave us a message during checkout to indicate which kit you need, and our team will process your order accordingly.

Variations

Product	Ex/Em (nm)	Detection Channels	Size Options	Catalog Number
ExoBrite™ 410/450 WGA EV Staining Kit	416/452 nm	Pacific Blue™	100 Labelings / 500 Labelings	30123-T / 30123
ExoBrite™ 490/515 WGA EV Staining Kit	490/516 nm	FITC	100 Labelings / 500 Labelings	30124-T / 30124
ExoBrite™ 560/585 WGA EV Staining Kit	562/584 nm	PE	100 Labelings / 500 Labelings	30125-T / 30125
ExoBrite™ 640/660 WGA EV Staining Kit	642/663 nm	APC	100 Labelings / 500 Labelings	30126-T / 30126