Bright, Sensitive EV Staining with Broad Compatibility

ExoBrite™ Annexin EV Staining Kits are optimally formulated conjugates of Annexin V, designed for bright and specific staining of purified extracellular vesicles (EVs) with minimal background. Unlike traditional lipophilic dyes that risk aggregation and false signals, ExoBrite™ Annexin stains bind specifically to phosphatidylserine (PS) residues on EV membranes, enabling accurate detection by flow cytometry.

Validated across 9 different cell line-derived EV sources (MCF-7, J774, U2OS, Jurkat, HeLa, Raji, CHO, U937, A549), ExoBrite™ Annexin EV Stains provide broad coverage, strong signal intensity, and low background interference, while remaining compatible with antibody co-staining for multi-parameter EV analysis.

Each kit includes ExoBrite™ Annexin EV Stain and 50X Annexin Binding Buffer for convenient experimental setup.

Key Features

• Optimally formulated Annexin V conjugates for EV detection
• Bright signal, low background, designed for flow cytometry
• Broad compatibility – stains EVs from 9 validated sources
• Compatible with antibody co-staining (e.g. CD9, CD63, CD81)
• Available in four colours for flexible experimental design
• Supplied with Annexin Binding Buffer

Notes

• ExoBrite™ Annexin EV Stains label EVs from all tested cell lines, but may not stain EVs from every source.

• For ExoBrite™ 490/515 dye, a biotin blocking step is recommended when using streptavidin-coated beads or surfaces to prevent nonspecific binding.

• Biotium products are available only in Singapore and Thailand.

✨ Discover ExoBrite™ Annexin EV Staining Kits today – four colours are available for your EV research.

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