Fluorescent Standards and Tracking Tools for EV Research

JOT-FLUO488-HRBCEV consists of a highly purified, pre-labelled population of human red blood cell-derived extracellular vesicles (RBCEVs) with a homogeneous size distribution (~50–250 nm). These EVs have undergone a stringent purification workflow to eliminate lipoproteins, serum proteins, and other impurities, ensuring clean and reproducible results.

The vesicles are fluorescently labelled with Alexa Fluor® 488 (excitation 488 nm, emission 496 nm), with the option to adapt to other compatible fluorophores (430, 561, 594, 647 channels). The labelling is stable, homogeneous, fixable, and highly photostable—ideal for reproducible tracking and calibration.

Applications include:

• ✅ Flow Cytometry – as a standard for nanoparticle detection or EV gating

• ✅ In Vitro Uptake Assays – monitor EV internalisation into target cells

• ✅ In Vivo Imaging – track EV biodistribution using IVIS and related platforms

• ✅ Calibration Tool – benchmark for fluorescent-based nanoparticle assays

Key Features & Benefits

• ✅ High Purity: Derived from pre-isolated RBCs; free from serum proteins and lipoproteins

• ✅ Stable Labelling: Homogeneous, fixable fluorophore conjugation without EV aggregation

• ✅ Photostability: Robust fluorescence signal across imaging modalities

• ✅ Versatile Applications: Compatible with microscopy, flow cytometry, IVIS, and other fluorescence-based detection methods

• ✅ Reproducibility: Reliable standard for EV tracking and assay calibration

Note: These products are distributed only in Singapore and Thailand.